Comparison between organic solvents in the urine of workers exposed to organic solvents and their solvent's metabolites

对低浓度尿液的保存方法进行了研究，建立了一种新的尿液保存方法。在新方法中，预先在瓶子中装入含有β-环糊精的尿液，并用铝封密封。工人接触甲苯、甲乙酮和异丙醇的混合溶剂，或甲苯、二甲苯和甲乙酮的混合溶剂，或二氯甲烷、1，2-二氯乙烷和三氯乙烯的混合溶剂，并测量接触混合溶剂的工人尿液中母体溶剂或代谢物的浓度。每种溶剂的环境浓度与工人尿中同一溶剂的尿液浓度之间存在密切的相关性，环境浓度与以肌酐浓度或比重校正的溶剂浓度之间的相关性较高。相反，环境中的溶剂浓度与尿中肌酐浓度或比重校正的代谢物浓度之间的相关系数高于环境浓度与未校正的代谢物浓度之间的相关系数。这些结果是通过假设溶剂通过简单的扩散从血液转移到尿液来解释的。因此，未校正的尿溶剂浓度与肌酐或比重校正的代谢物浓度之间的相关系数高于未校正的尿溶剂浓度与代谢物浓度的相关系数。

The method for preservation of urinary solvents of low concentration was examined and a new method was established. In the new method, the vials were filled with urine receiving beta cyclodextrin beforehand and sealed with aluminium seals.Workers were exposed to a mixed solvent of toluene, methylethylketone and isopropanol, or that of toluene, xylene and methyl ethyl ketone, or that of dichloromethane, 1,2-dichloroethane and trichloroethylene, and each solvent to which workers were simultaneously exposed and parent solvents or metabolites in the urine of workers exposed to the mixed solvents were measured.A close correlation was present between the ambient concentration of each solvent and the urinary concentration of the same solvent in the urine of workers showed a higher value than that between the ambient concentration and the solvent concentration corrected by creatinine concentration or by specific gravity. In contrast to this, the correlation coefficient between the ambient concentration of solvents and the corrected concentraion of metabolies by creatinine concentration or by specific gravity in the urine was higher than that between the ambient concentration and uncorrected concentration of metabolites. These results are explained by assuming that solvent is transferred from blood to urine by simple diffusion. Therefore, the correlation coefficient between the uncorrected concentration of urinary solvents and the metabolite concentrations corrected by creatinine or by specific gravity was higher than that between the uncorrected concentration of urinary solvents and uncorrected concentration of metabolite concentrations.