权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Preparation of specimens for external quality control of urinary biological monitoring

泌尿生物监测外部质量控制标本的制备

基本信息

批准号：
05557026
负责人：
OGATA Masana
金额：
$ 1.47万
依托单位：
Kawasaki University of Medical Welfare
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

Urines from normal human subjects who had not been exposed to organic solvents and artficial urines containing major components of human urine were prepared as specimens for the survey of quality control. Various concentrations of the hippuric acid, methylhippuric acid and mandelic acid were spiked into the normal and artificial urines. Some of specimens were freeze-dried and used for quality control studies.Thereafter, the specimens were kept at 30ﾟC,4ﾟC and -20ﾟC for 1,2 and 4 weeks, and the changes in concentrations of above described three acids during storage were analyzed by high performance liquid chromatography.In the case of artificial urines spiked with the three acids, the average concentration of each acid after storage was 97 per cent or more of that before storage under the condition at 30ﾟC for 1 week, 1ﾟC for 2 week and -20ﾟC for 4 weeks. The three acids were stable when they were kept at lower temperature and the mandelic acid was the most stable one among them. The cr … More eatinine was less stable compared to the three acids under the storing condition at 30ﾟC and 4ﾟC.In the case of normal urine spiked with three acids at high and low concentrations, the average concentration of each acid after storage was 99 per cent or more of that before storage under the condition at 30ﾟC for 1 week and 4ﾟC and -20ﾟC for 4 weeks.When artificial and normal urines spiked with three acids at high and low concentrations were freezedried, the average concentration of each acid after storage at 25ﾟC was approximately 100 per cent of that before storage in dried artificial urine and was 99 per cent or more of those before storage in dried human urine. The hippuric acid and methylhippuric acid in the urine of workers exposed to low concentrations of mixed toluene and xylenes could be kept without change for 6 months under the freezing condition at -20ﾟC.Urinaly metabolites of solvents contained in the specimens prepared for external quality control were proved to be stable after mailing at low temperature. Less

制备未接触有机溶剂的正常人类受试者的尿液和含有人类尿液主要成分的人工尿液作为质量控制调查的标本。将不同浓度的马尿酸、甲基马尿酸和扁桃酸掺入正常尿液和人工尿液中。部分样本被冷冻干燥并用于质量控制研究。随后，将样本在30℃、4℃和-20℃下保存1、2和4周，并通过高效液相色谱分析储存期间上述三种酸的浓度变化。对于掺有三种酸的人工尿液，储存后每种酸的平均浓度为该条件下储存前的97%或更多。 30°C 1 周，1°C 2 周，-20°C 4 周。这三种酸在较低温度下保持稳定，其中扁桃酸是最稳定的一种。与30°C和4°C保存条件下的三种酸相比，铬酸的稳定性较差。在正常尿液中添加高浓度和低浓度三种酸的情况下，在30°C保存1周和4°C和-20°C保存4周的条件下，保存后每种酸的平均浓度为保存前的99%或更多。高低三种酸冷冻干燥后，25°C 储存后每种酸的平均浓度约为干燥人造尿储存前的 100%，是干燥人尿储存前的 99% 或更多。接触低浓度混合甲苯和二甲苯的工人尿液中的马尿酸和甲基马尿酸在-20℃冷冻条件下可保存6个月无变化。外质控制备的标本中所含溶剂的尿液代谢物经低温邮寄后证明是稳定的。较少的