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Production of gene map using chromosome deletions in common wheat

利用普通小麦染色体缺失绘制基因图谱

基本信息

批准号：
02640499
负责人：
ENDO Takashi
金额：
$ 1.02万
依托单位：
Nara University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02640499/
关键词：
Common wheat Deletion mapping Deletion chromosome Gene map

项目摘要

The main purpose of this research was to produce a series of deletion stocks in a common wheat cultivar Chinese Spring, and it was mostly fulfilled by the study conducted from 1990 to 1991. A total of 382 deletion chromosomes were identified and isolated through the cytological selection by C-banding during several self-pollinated generations. 263 of them were in structually homozyhgous condition, in which there are a pair of a critical deletion and no corresponding normal homologue. These deletion stocks can be used for deletion mapping as they are. For the remaining deletions, however, homozygous plants were neither obtained nor fertile. Therefore, most of these heterozygous deletion stocks, in which there are one critical deletion and one corresponding normal homologue, were crossed with Chinese Spring nullisomic-tetrasomic stocks, which lack a pail, of chromosomes and have two pairs of a homoeologous chromosomes. From these Fl's, hemizygous plants, in which there is one critical deletion and no corresponding homologue, can be selected for deletion mapping. The deletions in the short arms of chromosome 2A and chromosoem 4B, whose homozygotes were sterile, have been maintained in heterozygou condition because no appropriate nullisomic-tetrasomics are available.Using the deletion stocks so far produced, deletion mappings with male-fertility gene and fertility-restoring gene against alien cytoplasm were conducted and the results wei, e published, or will be published soon. Other deletion mapping concerning some isozyrne genes and DNA markers are in progress.

本研究的主要目的是对普通小麦品种中国春进行一系列缺失库的培育，主要完成于1990~1991年的研究。在几个自花授粉世代中，通过细胞学C-显带选择，共鉴定和分离出382条缺失染色体。其中263个处于结构纯合状态，其中有一对临界缺失，没有相应的正常同源物。这些删除库可用于原样的删除映射。然而，对于剩下的缺失，纯合植株既没有获得，也没有生育能力。因此，这些杂合缺失群体中有一个关键缺失和一个对应的正常同源基因，大多数与中国春缺失-四体群体杂交，这些缺失群体缺乏一桶染色体，具有两对同源染色体。从这些F1中，可以选择其中有一个关键缺失而没有相应同源物的半合子植物进行缺失定位。由于没有合适的缺体-四体体，纯合子不育的2A和4B染色体短臂上的缺失一直保持在杂合子状态，利用目前已产生的缺失库，进行了雄性育性基因和育性恢复基因与异源细胞质的缺失作图，结果已发表或即将发表。关于一些同工酶基因和DNA标记的其他缺失定位正在进行中。