Production of barley chromosome dissection lines anddevelopment of PCR-based screening method

大麦染色体切割系的产生及基于PCR的筛选方法的开发

• 批准号: 20380006
• 负责人: ENDO Takashi
• 金额: $ 11.98万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380006/
• 关键词: オオムギ; コムギ; 染色体; 染色体地図; 染色体断片; Gcシステム; PCR; パンコムギ; ESTマーカー; 2H染色体; 6H染色体; 1H染色体; 細胞遺伝学

It was aimed to produce dissection lines of barley chromosomes(1H~ 7H) in commonwheat using the gametocidal(Gc) system and to develop a PCR-based method for theselection of the dissection lines. As a result, more than 50 dissection lines wereobtained for 2H, 3H, 4H, 5H and 7H, and effective PCR markers were found to detectthe presence of the individual dissected barley segments. Enough number ofdissection lines for 6H were not obtained and it was found difficult to apply the Gcsystem to 1H.

本研究旨在利用杀配子（Gc）系统获得普通小麦大麦染色体（1H~ 7 H）的分离系，并建立一种基于PCR的分离系筛选方法。结果，获得了2 H、3 H、4 H、5 H和7 H五个分离株系50多个，并找到了有效的PCR标记来检测大麦分离片段的存在。6 H的解剖线数目不够，而1H的GC系统很难应用。

项目成果

Dissection and cytological mapping of barley chromosome 2H in the genetic background of common wheat.

普通小麦遗传背景下大麦 2H 染色体的解剖和细胞学定位。

• DOI: 10.1266/ggs.86.231
• 发表时间: 2011
• 期刊: Genes & genetic systems
• 影响因子: 1.1
• 作者: G. Joshi;S. Nasuda;T. Endo
• 通讯作者: T. Endo

Gametocidal mapping : A method-throughput gene mapping inpost-genomics era. In : Chromosome Mapping Research Developments

杀配子作图：后基因组时代的方法通量基因作图。

• 发表时间: 2008
• 作者: Masoudi-Nejad A;Endo TR
• 通讯作者: Endo TR

A fine cytological mapping of rye chromosome 1R

黑麦染色体 1R 的精细细胞学图谱

• 发表时间: 2011
• 作者: Kurauchi;et al.;Endo TR
• 通讯作者: Endo TR

Cytological Dissection of the Triticeae Chromosomes by the Gametocidal System

• DOI: 10.1007/978-1-61737-957-4_14
• 发表时间: 2011-01-01
• 期刊: PLANT CHROMOSOME ENGINEERING
• 作者: Endo, Takashi R.

Nasuda SEstablishment of 14 wheat lines carrying telosomes of barley chromosome

Nasuda SE 建立 14 个携带大麦染色体端粒的小麦品系

• 期刊: 7 HeWIS
• 作者: Sakai K;Endo TR
• 通讯作者: Endo TR