Physical mapping and molecular genetic study of barley chromosomes by genetic genome rearrangement
Physical mapping and molecular genetic study of barley chromosomes by genetic genome rearrangement
批准号:
08406001
负责人:
ENDO Takashi
金额:
$18.3万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (A)
财政年份:
1996
资助国家:
日本
项目状态:
已结题
起止时间:
1996 至 1999
中文摘要
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英文摘要
Aiming to construct physical maps of barley chromosomes based on various DNA markers, we conducted the present study to induce structural changes in barley chromosomes added to common wheat by the use of gametocidal chromosomes. The results obtained during the research period between 1996 and 2000 are as follows.1. Production of Barley Addition Lines Generating Chromosomal Structural ChangesWe introduced two gametocidal chromosomes, one from Aegilops cylindrica, one from Ae. triuncialis, into barley chromosome addition lines of common wheat by crossing and cytological screening.2. Development of Technique Identifying Barley Chromatin Based on Chromosome Banding and Fluorescence In Situ HybridizationCombining chromosome banding (C-banding and N-banding) and in situ hybridization (FISH using a probe of the barley-specific subtelomeric repeat and GISH using a probe of the total barley genomic DNA), we developed a technique by which we can identify barley chromatin in the genomic background of common wheat.3. Identification and Isolation of Barley Chromosomal Structural ChangesUsing the lines and technique mentioned above, we demonstrated the gametocidal chromosomes can induce structural changes in every barley chromosome with high frequencies.4. Physical Mapping of a Barley ChromosomeWe screened many deletions and translocations in barley chromosome 7H and conducted deletion mapping with them based on RFLP and AFLP markers, demonstrating the efficacy of the barley chromosomal structural changes generated in this study.
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Endo, T.R.: "Allocation of a gametocidal chromosomes of Aegilops cylindrica to wheat homoeologous group2."Genes & Genetic Systems. 71. 243-246 (1996)
Endo, T.R.:“将圆柱山羊草的杀配子染色体分配给小麦同源组 2。”基因
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Yoshino,M.: "Detection of terminal deletions in barley chromosomes by the PCR-based method." Genes & Genetic Systems. 73. 163-166 (1998)
Yoshino,M.:“通过基于 PCR 的方法检测大麦染色体末端缺失。”
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Zhang H.: "Identification of AFLP fragments on chromosome 1BS of common wheat as DNA markers associated with the fertility-restoring gene (Rfv1)"Genome. (in press).
张 H.:“普通小麦 1BS 染色体上 AFLP 片段的鉴定,作为与育性恢复基因 (Rfv1) 相关的 DNA 标记”基因组。
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通讯作者:
Endo,T.R.: "Allocation of a gametocidal chromosome of Aegilops cylindrica to wheat homoeologous goup 2" Genes & Genetic Systems. 71. 243-246 (1996)
Endo,T.R.:“圆柱山羊草杀配子染色体到小麦同源组 2 的分配”基因
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Schubert,I.: "An efficient screening for terminal deletions and translocations of barley chromosomes added to common wheat." The Plant Journal. 14. 489-495 (1998)
Schubert,I.:“对添加到普通小麦中的大麦染色体的末端缺失和易位进行有效筛选。”
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共 18 条
Introduction of alien genes into common wheat by means of homoeologous pairing induced by B chromosomes
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批准号:23658009
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.41万
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财政年份:2011
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负责人:ENDO Takashi
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依托单位:
Production of barley chromosome dissection lines anddevelopment of PCR-based screening method
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批准号:20380006
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.98万
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财政年份:2008
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负责人:ENDO Takashi
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依托单位:
Introduction of the clusters of genes controlling pest and disease resistance from related crops to common wheat by means of the genetic genome rearrangement
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批准号:12306001
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$29.96万
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财政年份:2000
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负责人:ENDO Takashi
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依托单位:
Development of a genetic system to introduce alien useful genes into common wheat and to determine their loci
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批准号:07556132
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$1.66万
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财政年份:1995
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负责人:ENDO Takashi
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依托单位:
Production of deletion stocks and deletion mapping in common wheat
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批准号:05660008
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.34万
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财政年份:1993
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负责人:ENDO Takashi
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依托单位:
Production of gene map using chromosome deletions in common wheat
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批准号:02640499
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.02万
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财政年份:1990
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负责人:ENDO Takashi
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依托单位:
海外基金