Amphiphile-induced incorporation of a substance into cells

研究了两亲体诱导的四乙基铵离子（TEA^+）摄取到人红细胞中的情况，并观察了细胞形状的变化。采用TEA^+敏感电极测定吸收量。离子敏感电极的使用具有简单、容易的固有优点，特别是能够在不与测定混合物分离的情况下进行检测。结果发现，当两亲细胞将细胞形态转变为内陷形态时，TEA^+优先并入红细胞。这与细胞外乙酰胆碱酯酶的释放形成对比，这种释放在两亲体中明显发生，导致圆齿状。提示红细胞膜内陷刺激了液泡的形成，其中存在于外界介质中的TEA^+被捕获。通过本研究，我们进一步阐明了生物活性肽诱导人红细胞形状变化的机制，并建立了一种新的测定腺苷-5'-三磷酸腺苷（ATP）的方法和测定膜上形成通道大小的电位法。这些结果都已发表，可以通过本文后面的参考文献看到。

Amphiphile-induced tetraethylammonium ion (TEA^+) uptake into human erythrocytes was examined along with cell shape change. A TEA^+-sensitive electrode was used to determine the amount of uptake. The use of an ion-sensitive electrode has the inherent advantage of being simple, easy, and especially capable of detection without separation from assay mixtures. It was found that TEA^+ was preferentially incorporated into erythrocytes when amphiphiles changed cell shape to an invaginated form. This was contrasted with the release of acetylcholinesterase outside cells which occurred markedly with the amphiphiles, causing the crenated form. It was suggested that the invagination of erythrocyte membrane stimulated the formation of vacuoles, in which TEA^+ existing in an external medium was entrapped.Through this research, we further elucidated the mechanism of shape changes of human erythrocytes induced by biologically active peptides and developed a new method to determine adenosine-5'-triphosphate (ATP) and to determine a channel size formed in membrane by a potentiometric method. These results were all published and can be seen through references described on the back of this paper.