Amphiphile-induced incorporation of a substance into cells

两亲物诱导的四乙基铵离子（TEA^+）进入人红细胞的摄取沿着细胞形状的变化。使用TEA^+敏感电极来测定摄取量。离子敏感电极的使用具有简单、容易并且特别能够在不与测定混合物分离的情况下进行检测的固有优点。结果发现，当两亲物将细胞形状改变为内陷形式时，TEA^+优先掺入红细胞。这与乙酰胆碱酯酶在细胞外的释放形成对比，乙酰胆碱酯酶在两亲物中明显发生，导致锯齿状形式。本研究进一步阐明了生物活性肽诱导人红细胞形态改变的机理，并建立了一种新的测定腺苷三磷酸（ATP）和膜通道大小的电位滴定法。这些结果都已发表，可以通过本文背面描述的参考文献看到。

Amphiphile-induced tetraethylammonium ion (TEA^+) uptake into human erythrocytes was examined along with cell shape change. A TEA^+-sensitive electrode was used to determine the amount of uptake. The use of an ion-sensitive electrode has the inherent advantage of being simple, easy, and especially capable of detection without separation from assay mixtures. It was found that TEA^+ was preferentially incorporated into erythrocytes when amphiphiles changed cell shape to an invaginated form. This was contrasted with the release of acetylcholinesterase outside cells which occurred markedly with the amphiphiles, causing the crenated form. It was suggested that the invagination of erythrocyte membrane stimulated the formation of vacuoles, in which TEA^+ existing in an external medium was entrapped.Through this research, we further elucidated the mechanism of shape changes of human erythrocytes induced by biologically active peptides and developed a new method to determine adenosine-5'-triphosphate (ATP) and to determine a channel size formed in membrane by a potentiometric method. These results were all published and can be seen through references described on the back of this paper.