Amphiphile-induced incorporation of a substance into cells

研究了两亲分子诱导的人红细胞对四乙基铵离子(TEA)的摄取以及细胞形态的变化。用TEA敏感电极测定摄取量。使用离子敏感电极具有简单、容易的固有优点，特别是能够在不从分析混合物中分离的情况下进行检测。结果发现，当两亲分子改变细胞形状为内陷形式时，TEA^优先结合到红细胞中。这与乙酰胆碱酯酶在细胞外的释放形成鲜明对比，细胞外释放的乙酰胆碱酯酶明显地与两亲分子发生在一起，导致了皱折的形式。通过本研究，我们进一步阐明了生物活性多肽诱导人红细胞形态变化的机制，并建立了用电位法测定红细胞膜上三磷酸腺苷(ATP)和膜上形成的通道大小的新方法。这些结果都已发表，并可通过本文背面描述的参考文献查看。

Amphiphile-induced tetraethylammonium ion (TEA^+) uptake into human erythrocytes was examined along with cell shape change. A TEA^+-sensitive electrode was used to determine the amount of uptake. The use of an ion-sensitive electrode has the inherent advantage of being simple, easy, and especially capable of detection without separation from assay mixtures. It was found that TEA^+ was preferentially incorporated into erythrocytes when amphiphiles changed cell shape to an invaginated form. This was contrasted with the release of acetylcholinesterase outside cells which occurred markedly with the amphiphiles, causing the crenated form. It was suggested that the invagination of erythrocyte membrane stimulated the formation of vacuoles, in which TEA^+ existing in an external medium was entrapped.Through this research, we further elucidated the mechanism of shape changes of human erythrocytes induced by biologically active peptides and developed a new method to determine adenosine-5'-triphosphate (ATP) and to determine a channel size formed in membrane by a potentiometric method. These results were all published and can be seen through references described on the back of this paper.