Amphiphile-induced incorporation of a substance into cells

两亲物诱导物质掺入细胞

• 批准号: 03671026
• 负责人: KATSU Takashi
• 金额: $ 1.28万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03671026/
• 关键词: Amphiphile; Membrane permeability; Erythrocyte shape; Shape change; Ion-selective electrode; Adenosine-5'-triphosphate; Biologically active peptide; Drug-membrane interaction; アデノシン-5'-三リン酸; 赤血球膜; 構造活性相間

Amphiphile-induced tetraethylammonium ion (TEA^+) uptake into human erythrocytes was examined along with cell shape change. A TEA^+-sensitive electrode was used to determine the amount of uptake. The use of an ion-sensitive electrode has the inherent advantage of being simple, easy, and especially capable of detection without separation from assay mixtures. It was found that TEA^+ was preferentially incorporated into erythrocytes when amphiphiles changed cell shape to an invaginated form. This was contrasted with the release of acetylcholinesterase outside cells which occurred markedly with the amphiphiles, causing the crenated form. It was suggested that the invagination of erythrocyte membrane stimulated the formation of vacuoles, in which TEA^+ existing in an external medium was entrapped.Through this research, we further elucidated the mechanism of shape changes of human erythrocytes induced by biologically active peptides and developed a new method to determine adenosine-5'-triphosphate (ATP) and to determine a channel size formed in membrane by a potentiometric method. These results were all published and can be seen through references described on the back of this paper.

研究了两亲分子诱导的人红细胞对四乙基铵离子(TEA)的摄取以及细胞形态的变化。用TEA~(2+)敏感电极测定摄取量。使用离子敏感电极具有简单、容易的固有优点，特别是能够在不从分析混合物中分离的情况下进行检测。研究发现，当两亲分子改变细胞形状为内陷形式时，TEA~+优先进入红细胞。这与乙酰胆碱酯酶在细胞外的释放形成鲜明对比，细胞外释放的乙酰胆碱酯酶明显地与两亲分子发生在一起，导致了皱折的形式。通过本研究，我们进一步阐明了生物活性多肽诱导人红细胞形态变化的机制，并建立了用电位法测定红细胞膜上三磷酸腺苷(ATP)和膜上形成的通道大小的新方法。这些结果都已发表，并可通过本文背面描述的参考文献查看。

项目成果

Takashi KATSU: "Potentiometric method for the determination of adebno-sine-5'-triphosphate" Analytica Chimica Acta. 276. 373-376 (1993)

Takashi KATSU：“测定 5-三磷酸腺苷的电位法”Analytica Chimica Acta。

Takashi KATSU: "The use of tetraalkylammonium ion-sensitive electrodes for the liposome marker release assay" Analytical Chemistry. 65. 176-180 (1993)

Takashi KATSU：“使用四烷基铵离子敏感电极进行脂质体标记物释放测定”分析化学。

Takashi Katsu: "Determination of cholinesterase in blood serum with a benzoate-sansitive membrane electrode" Anal. Chim. Acta. 254. 95-97 (1991)

Takashi Katsu：“用苯甲酸盐敏感膜电极测定血清中的胆碱酯酶” Anal。

Takashi Katsu: "Potentiometric method for the determination of adenosine-5'-triphosphate" Analytica Chimica Acta. ?. (1993)

Takashi Katsu：“测定 5-三磷酸腺苷的电位法”Analytica Chimica Acta。

Takashi KATSU: "Mode of action of antimicrobial peptide,tachyplesin I,on biomembranes" Biological and Pharmaceutical Bulletin. 16. 178-181 (1993)

Takashi KATSU：“抗菌肽，鲎素 I，在生物膜上的作用模式”生物和药物通报。