Development of drug electrodes with high sensitivity and selectivity

高灵敏度、高选择性药物电极的研制

基本信息

  • 批准号:
    10672019
  • 负责人:
  • 金额:
    $ 2.24万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1998
  • 资助国家:
    日本
  • 起止时间:
    1998 至 2000
  • 项目状态:
    已结题

项目摘要

The development and application of ion-selective electrodes continue to be an area of focus. In this study, special attention was paid to develop organic ammonium ion-selective membrane electrodes, because there are many drugs having the amino group in their molecules. First, we constructed biogenic amine-sensitive membrane electrode using the combinations of appropriate solvent mediators and a lipophilic ion-exchanger, sodium tetrakis [3,5-bis (2-methoxyhexafluoro-2-propyl) phenyl] borate. It was found solvent mediators, such as thiophosphate esters with a P=S group, generally strengthened response to organic ammonium ions including histamine, and markedly suppressed responses to inorganic cations, such as Na^+ and K^+, suggesting that hydrogen bonding between the NH_3^+ group of organic ammonium ions and the negatively polarized sulfur atom in the P=S group would enhance such responses. We applied the histamine electrode constructed using prothiofos, one of the compounds containing a … More P=S group, to determine histamine release from mast cells. The histamine concentrations determined agreed well with those determined by an established fluorimetric assay.Second, we constructed a triethylammonium-sensitive membrane electrode using sodium tetrakis [3,5-bis (2-methoxyhexafluoro-2-propyl) phenyl] borate as an ion-exchanger and benzyl 2-nitrophenyl ether as a solvent mediator and applied it for the determination of the pH difference across a cell membrane. The method is based on monitoring of the pH gradient-induced uptake of triethylammonium in situ. In experiments using liposomes, the uptake of triethylammonium into liposomes was quantitatively induced according to the pH difference across the liposomal membrane. The transmembrane pH difference in Escherichia coli cells and the light-induced formation of the pH difference across the envelop vesicles of Halobacterium halobium were successfully determined by the present method.We described here only two results. Other results can be seen through references described in the back of this report. Less
离子选择性电极的开发和应用仍然是一个焦点领域。由于许多药物分子中含有氨基,因此本研究特别关注有机铵离子选择性膜电极的研制。首先,我们使用适当的溶剂介体和亲脂性离子交换剂四[3,5-二(2-甲氧基六氟-2-丙基)苯基]硼酸钠的组合构建了生物胺敏感膜电极。结果表明,溶剂介质如含P=S基团的硫代磷酸酯一般能增强对有机铵离子(包括组胺)的反应,而对无机阳离子(如Na^+和K^+)的反应则有明显的抑制作用,这表明有机铵离子的NH_3^+基团与P=S基团中负极性的硫原子之间的氢键作用能增强这种反应。我们应用了由丙硫磷(prothiofos)构建的组胺电极,丙硫磷是一种含有 ...更多信息 P=S组,测定肥大细胞释放组胺的量。其次,我们以四[3,5-双(2-甲氧基六氟-2-丙基)苯基]硼酸钠为离子交换剂,苄基2-硝基苯基醚为溶剂介质,构建了三乙胺敏感膜电极,并将其用于测定细胞膜上的pH值。该方法是基于监测的pH梯度诱导的摄取三乙铵原位。在使用脂质体的实验中,根据脂质体膜上的pH差定量诱导三乙基铵摄入脂质体。用本方法成功地测定了大肠杆菌(Escherichia coli)细胞跨膜pH差和盐生盐杆菌(Halobacterium halobium)光诱导的跨膜pH差。其他结果可参见本报告后面所述的参考文献。少

项目成果

期刊论文数量(18)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takashi Katsu: "Ion-selective electrode for transmembrane pH difference measurements"Analytical Chemistry. (in press). (2001)
Takashi Katsu:“用于跨膜 pH 差异测量的离子选择电极”分析化学。
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    0
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Takashi Katsu: "Mexiletine-sensitive membrane electrode for medical application"Journal of Pharmaceutical and Biomedical Analysis. 19. 585-593 (1999)
Takashi Katsu:“医疗应用美西律敏感膜电极”《药物与生物医学分析杂志》。
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    0
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Dafeng Xu: "Lead-selective membrane electrode based on dibenzyl phosphate"Analytica Chimica Acta. 401. 111-115 (1999)
徐大峰:“基于磷酸二苄酯的铅选择性膜电极”Analytica Chimica Acta。
  • DOI:
  • 发表时间:
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  • 影响因子:
    0
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Takashi Katsu: "Ion-selective electrode for transmembrane pH difference measurements"Anal.Chem. (in press). (2001)
Takashi Katsu:“用于跨膜 pH 差异测量的离子选择电极”Anal.Chem.
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Takashi Katsu: "Determination of histamine release from mast cells using a histamine-sensitive membrane electrode"Analytica Chimica Acta. 396. 189-193 (1999)
Takashi Katsu:“使用组胺敏感膜电极测定肥大细胞释放的组胺”Analytica Chimica Acta。
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    0
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KATSU Takashi其他文献

KATSU Takashi的其他文献

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{{ truncateString('KATSU Takashi', 18)}}的其他基金

In situ monitoring of drug action using electrochemical sensors
使用电化学传感器原位监测药物作用
  • 批准号:
    25460036
  • 财政年份:
    2013
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
New development of studies on the interaction between cell membranes and drugs using sensors
利用传感器研究细胞膜与药物相互作用的新进展
  • 批准号:
    22590036
  • 财政年份:
    2010
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development and application of sensors selective to biologically active substances
生物活性物质选择性传感器的研制及应用
  • 批准号:
    19590039
  • 财政年份:
    2007
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development and application of high-performance ion-selective electrodes
高性能离子选择电极的开发及应用
  • 批准号:
    16590027
  • 财政年份:
    2004
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of electrochemical sensors responding to biologically active substances
开发响应生物活性物质的电化学传感器
  • 批准号:
    08672477
  • 财政年份:
    1996
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development and application of ion-selective electrodes
离子选择电极的研制及应用
  • 批准号:
    06672144
  • 财政年份:
    1994
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Amphiphile-induced incorporation of a substance into cells
两亲物诱导物质掺入细胞
  • 批准号:
    03671026
  • 财政年份:
    1991
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Mechanism of Biological Membrane Damage Induced by Amphiphilic Drugs and Peptides
两亲性药物和肽诱导生物膜损伤的机制
  • 批准号:
    01571178
  • 财政年份:
    1989
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Action mechanism of cationic drugs increasing the permeability of an outer membrane of Gram-negative bacteria
阳离子药物增加革兰氏阴性菌外膜通透性的作用机制
  • 批准号:
    62570967
  • 财政年份:
    1987
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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职业:通过扫描隧道电位法揭示强相关材料中电荷载流子的动力学
  • 批准号:
    2239478
  • 财政年份:
    2023
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The real space observation of non-local electrical conductivity by low temperature scanning tunneling potentiometry
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    16K04956
  • 财政年份:
    2016
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Scanning tunneling potentiometry measurements on surface/ultrathin superconductors
表面/超薄超导体的扫描隧道电位测量
  • 批准号:
    16K13683
  • 财政年份:
    2016
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of scanning nonlinear dielectric potentiometry and its applications to electronic materials and devices evaluation
扫描非线性介电电位法的发展及其在电子材料与器件评价中的应用
  • 批准号:
    15K04673
  • 财政年份:
    2015
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of a spin-polarized scanning tunneling potentiometry system and its application to micro fabricated surface Rashba systems
自旋极化扫描隧道电位系统的开发及其在微加工表面Rashba系统中的应用
  • 批准号:
    23686007
  • 财政年份:
    2011
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Young Scientists (A)
Recognition of Inorganic Anions by Hydrogen Bonding Ionophores and Their Use for Ion-Selective Potentiometry
氢键离子载体对无机阴离子的识别及其在离子选择电位测定中的应用
  • 批准号:
    10640584
  • 财政年份:
    1998
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Deveropment of a continuous monitoring system for an acid rain based on null-point potentiometry
基于零点电位法的酸雨连续监测系统的研制
  • 批准号:
    07640801
  • 财政年份:
    1995
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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