Development of drug electrodes with high sensitivity and selectivity

高灵敏度、高选择性药物电极的研制

基本信息

批准号：
10672019
负责人：
KATSU Takashi
金额：
$ 2.24万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

The development and application of ion-selective electrodes continue to be an area of focus. In this study, special attention was paid to develop organic ammonium ion-selective membrane electrodes, because there are many drugs having the amino group in their molecules. First, we constructed biogenic amine-sensitive membrane electrode using the combinations of appropriate solvent mediators and a lipophilic ion-exchanger, sodium tetrakis [3,5-bis (2-methoxyhexafluoro-2-propyl) phenyl] borate. It was found solvent mediators, such as thiophosphate esters with a P=S group, generally strengthened response to organic ammonium ions including histamine, and markedly suppressed responses to inorganic cations, such as Na^+ and K^+, suggesting that hydrogen bonding between the NH_3^+ group of organic ammonium ions and the negatively polarized sulfur atom in the P=S group would enhance such responses. We applied the histamine electrode constructed using prothiofos, one of the compounds containing a … More P=S group, to determine histamine release from mast cells. The histamine concentrations determined agreed well with those determined by an established fluorimetric assay.Second, we constructed a triethylammonium-sensitive membrane electrode using sodium tetrakis [3,5-bis (2-methoxyhexafluoro-2-propyl) phenyl] borate as an ion-exchanger and benzyl 2-nitrophenyl ether as a solvent mediator and applied it for the determination of the pH difference across a cell membrane. The method is based on monitoring of the pH gradient-induced uptake of triethylammonium in situ. In experiments using liposomes, the uptake of triethylammonium into liposomes was quantitatively induced according to the pH difference across the liposomal membrane. The transmembrane pH difference in Escherichia coli cells and the light-induced formation of the pH difference across the envelop vesicles of Halobacterium halobium were successfully determined by the present method.We described here only two results. Other results can be seen through references described in the back of this report. Less

离子选择电极的开发和应用仍然是重点的领域。在这项研究中，特别注意开发有机铵离子选择性膜电极，因为它们的分子中有许多药物具有氨基氨基。首先，我们使用适当的溶剂介质和亲脂性离子 - 脱糖剂的组合构建了生物胺敏感的膜电极，四甲基钠[3,5-双基（2-甲氧基hexafluoro-2-丙基）苯基]苯基。 It was found solvet mediators, such as thiophosphate esters with a P=S group, generally strengthened response to organic ammonium ions including histamine, and markedly suppressed responses to inorganic cations, such as Na^+ and K^+, suggesting that hydrogen bonding between the NH_3^+ group of organic ammonium ions and the negatively polarized sulfur atom in the P=S group would enhance such responses.我们应用了使用丙座式构造的组胺电极，丙二醇是包含A…更多P = S组的一种化合物，以确定从肥大细胞中释放组胺。 The histamine concentrations determined well with those determined by an established fluorimetric assay.Second, we constructed a triethylammonium-sensitive membrane electrode using sodium tetrakis [3,5-bis (2-methoxyhexafluoro-2-propyl) phenyl] borate as an ion-exchanger and benzyl 2-nitrophenyl ether as a solvent mediator and applied it for the在细胞膜上的pH差异测定。该方法基于监测pH梯度诱导的原位摄取的摄取。在使用脂质体的实验中，根据整个脂质体的pH差异诱导三乙基铵对脂质体的摄取。大肠杆菌细胞中的跨膜pH差异和在卤素卤烟片中的pH差异的光诱导的形成是通过本方法成功确定的。我们在这里仅描述了两个结果。其他结果可以通过本报告背面所述的参考文献来看待。较少的