Studies on the genes controlling lethality and development of the silkmoth, Bombyx mori.

控制家蚕致死和发育的基因研究。

• 批准号: 04404010
• 负责人: KOBAYASHI Masahiko
• 金额: $ 13.12万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04404010/
• 关键词: Lethal gene; Chromosome; Genome; Neuropeptide; RAPD; Linkage map; Mutation; Bombyx mori; 胚子発生; 休眠; ペプチドホルモン; ゲノム解析; 遺伝的モザイク; 家蚕; 前胸腺; ホメオティック遺伝子; PCR; エクダイソン

We adopted genome mapping as a strategy to analyze lethal genes in the silkworm, Bombyx mori since we thought positional cloning would be the most direct pathway to access the lethal genes and other developent-controlling genes. 1. We constructed an integrated map of RAPDs, RFLPs and phenotypic loci on chromosome 22, by mating experiments. This map contains the sku lethal gene. 2. A development-controlling gene, the DH-PBAN gene, was mapped at -2.5cM on chromosome 22.3. The RAPD linkage map was corresponded to the conventional phenotypic map for chromosomes 7,13 and 18 as linkage groups. Some lethal genes such as Obs, are carried by these chromosomes. 4. A few RAPDs on the Z chromosome were cloned and sequenced. One of them was considered to be a single copy sequence. 5. Some RAPDs on chromosomes 7 and 13 were cloned and sequenced. 8. A major factor which control the life span and egg-laying behavior in strain xu20, was determined to be linked to a RAPD on the 29th RAPD linkage group. 6. Sequence divergence at synonymous and non-synonymous sites of the aryphorin gene was estimated from the bombycid and saturniid silkmoths. A phylogenetic tree based on the non-synonymous sites of the gene was proposed as an evolutionary relationship of silkmoths. 7. Messenger RNA for hexamerins were quantitated along develoment and between sexes in Samia cynthia ricini and Antheraea pernyi. 8. The polyhedrin genes of the B.mori nuclear polyhedrosis virus were sequenced for an amorphous polyhedron mutant and a cuboidal polyhedron mutant. We revealed several amino acid substitutions in the polyhedrin-encoding region.

我们采用基因组作图作为分析家蚕致死基因的策略，因为我们认为定位克隆将是获取致死基因和其他发育控制基因的最直接途径。 1.通过交配实验，我们构建了22号染色体上的RAPD、RFLP和表型位点的整合图谱。该图谱包含 sku 致死基因。 2.发育控制基因DH-PBAN基因定位于22.3号染色体上的-2.5cM处。 RAPD连锁图对应于作为连锁群的7,13和18号染色体的常规表型图。一些致命基因（例如 Obs）由这些染色体携带。 4. Z染色体上的一些RAPD被克隆并测序。其中一个被认为是单拷贝序列。 5.对7号和13号染色体上的一些RAPD进行克隆和测序。 8.确定控制菌株xu20的寿命和产卵行为的一个主要因素与第29个RAPD连锁群上的RAPD有关。 6.从家蚕和土蚕中估计了 aryphorin 基因同义和非同义位点的序列分歧。提出了基于基因非同义位点的系统发育树作为蚕蛾的进化关系。 7. 对蓖麻蚕和柞蚕的发育过程中和两性之间的六聚体信使 RNA 进行定量。 8.对家蚕核多角体病毒的多角体蛋白基因进行了测序，以获得无定形多角体突变体和立方体多角体突变体。我们揭示了多角体蛋白编码区域中的几个氨基酸取代。

项目成果

洗 幸夫,小林 正彦: "家蚕休眠卵の非休眠化におよぼすコロナ放電の影響" 日本蚕糸学雑誌. 62(1). (1993)

Yukio Arashi，Masahiko Kobayashi：“电晕放电对家蚕休眠卵非休眠的影响”日本蚕业杂志 62（1）（1993）。

Toru Shimada et al.: "Polymorphism and linkage analysis of the prothoracicotropic hormone gene in the silkmoth" Bombyx mori.Genetical Research (Cambridge). 63 (4). 190-195 (1994)

Toru Shimada 等人：“蚕蛾促前胸腺激素基因的多态性和连锁分析”。家蚕。遗传学研究（剑桥）。

永田 昌男,土田 卓三,折笠 千登世,鈴木 昭憲: "光沢不眠蚕のエクジステロイド分泌系にいつて" 日本蚕糸学雑誌. 61(5). 400-406 (1992)

Masao Nagata，Takuzo Tsuchida，Chitoyo Orikasa，Akinori Suzuki：“光泽失眠蚕的蜕皮类固醇分泌系统简介”日本蚕丝学杂志 61（5）（1992）。

Toru Shimada et al.: "Polymorphism and linkage analysis of the prothoracicotropic hormone gene in the silkmoth,Bombyx mori." Genetical Research. 63(4). 190-195 (1994)

Toru Shimada 等：“蚕蛾促前胸腺激素基因的多态性和连锁分析。”

Amornrat Promboon et al.: "Linkage map of random amplified polymorphic DNAs (RAPDs) in the Silkworm,Bombyx mori." Genetical Research. 65(3)(in press). (1995)

Amornrat Promboon 等人：“家蚕中随机扩增多态性 DNA (RAPD) 的连锁图。”