FT-Mass Spectrometer with two UHPLC Systems

我们申请了最新一代的质谱仪，与纳米-超高压液相色谱仪(Nano-UHPLC)系统相耦合。该设备将取代LC-MS系统(2011年购买)，后者由于其老化和低测序速度而不再具有竞争力。所考虑的质谱仪将用于复杂多肽混合物的分析，并且必须具有高灵敏度、测序速度和质量准确度。此外，我们还申请了一种专用的超高效液相系统，该系统将用于复杂多肽混合物的离线分离(分级)。申请的设备将增加生产能力，更新设备园区，并在分析通用性方面扩展Proteom Centrum Tübingen(PCT)现有的质谱学专业知识。这些设备将用于PCT自己的研究项目，并在Tübingen大学蛋白质组学平台(PxP)的框架内使用。自2003年以来，作为蛋白质组学核心基金，PCT已经参与了1,125多个合作项目，并为233份出版物做出了贡献。建议的设备将用于进一步开发定量质谱学领域的方法，并在大量正在进行的合作中应用。因此，自己、地方、国家和国际合作项目将得到最新的MS和UHPLC技术的支持。

We apply for a mass spectrometer of the latest generation, coupled to a nano-ultra-high-pressure liquid chromatograph (nano-UHPLC) system. The device will replace an LC-MS system (year of purchase 2011), which is no longer competitive due to its age and low sequencing speed. The considered mass spectrometer will be employed in analysis of complex peptide mixtures and must deliver high sensitivity, sequencing speed and mass accuracy. In addition, we apply for a dedicated UHPLC system that will be used for offline separation (fractionation) of complex peptide mixtures. The requested devices will increase the throughput, renew the equipment park and expand the mass spectrometric expertise existing at the Proteom Centrum Tübingen (PCT) in terms of versatility of analysis.The devices will be used in own research projects of the PCT and within the framework of the Proteomics Platform (PxP) of the University of Tübingen. As a Proteomics Core Facility since 2003, the PCT has worked on over 1,125 collaborative projects and contributed to 233 publications. The proposed devices will be used for the further development of methods in the field of quantitative mass spectrometry and applied in a large number of ongoing collaborations. Own, local, national and international cooperation projects will therefore be supported with the latest MS and UHPLC technology.