FT-Mass Spectrometer with two UHPLC Systems

配备两个 UHPLC 系统的 FT 质谱仪

• 批准号: 525521526
• 金额: --
• 依托单位: Eberhard Karls Universität Tübingen
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/525521526?language=en
• 关键词: FT; Mass; Spectrometer; two; UHPLC

We apply for a mass spectrometer of the latest generation, coupled to a nano-ultra-high-pressure liquid chromatograph (nano-UHPLC) system. The device will replace an LC-MS system (year of purchase 2011), which is no longer competitive due to its age and low sequencing speed. The considered mass spectrometer will be employed in analysis of complex peptide mixtures and must deliver high sensitivity, sequencing speed and mass accuracy. In addition, we apply for a dedicated UHPLC system that will be used for offline separation (fractionation) of complex peptide mixtures. The requested devices will increase the throughput, renew the equipment park and expand the mass spectrometric expertise existing at the Proteom Centrum Tübingen (PCT) in terms of versatility of analysis.The devices will be used in own research projects of the PCT and within the framework of the Proteomics Platform (PxP) of the University of Tübingen. As a Proteomics Core Facility since 2003, the PCT has worked on over 1,125 collaborative projects and contributed to 233 publications. The proposed devices will be used for the further development of methods in the field of quantitative mass spectrometry and applied in a large number of ongoing collaborations. Own, local, national and international cooperation projects will therefore be supported with the latest MS and UHPLC technology.

我们申请了最新一代的质谱仪，与纳米超高压液相色谱仪（纳米UHPLC）系统相结合。该设备将取代LC-MS系统（2011年购买），该系统因其老化和低测序速度而不再具有竞争力。所考虑的质谱仪将用于分析复杂的肽混合物，并且必须提供高灵敏度、测序速度和质量准确度。此外，我们还申请了专用UHPLC系统，用于复杂肽混合物的离线分离（分馏）。所请求的设备将增加吞吐量、更新设备园区并在分析的多功能性方面扩展图宾根蛋白质中心（PCT）现有的质谱专业知识。这些设备将用于PCT自己的研究项目以及图宾根大学蛋白质组学平台（PxP）的框架内。自2003年以来，作为蛋白质组学核心机构，PCT已参与了超过1，125个合作项目，并发表了233篇论文。所提出的设备将用于定量质谱领域方法的进一步开发，并应用于大量正在进行的合作。因此，最新的MS和UHPLC技术将支持自己的，当地的，国家和国际合作项目。