Acquisition of a Thermo Scientific Orbitrap XL Hybrid FT Mass Spectrometer

描述(由申请人提供)：本建议书要求提供资金，用于购买Thermo Science LTQ Orbitrap混合FT质谱仪和数据系统。该仪器直接连接到提供LC/MS和(MS)n功能的Dionex Nano高效液相系统，并将安装在石溪医学院的蛋白质组学中心。该系统将提供高分辨率、精确的质量测量和电子转移解离这两项新的质谱仪技术，这两项技术将显著促进石溪大学(SBU)13名杰出科学家的研究目标和实验室生产率，他们是主要用户群体。这些方法目前无法在SBU获得，也无法从其他组织获得或难以从其他组织获得。LC/MS/MS在表征具有重要生物医学意义的分子方面是无与伦比的，包括脂类、多肽、寡核苷酸、寡糖等生物聚合物以及非挥发性、不稳定或热不稳定的较小化合物。通过电喷雾或大气压化学电离源将液相色谱仪与质谱仪耦合的技术现在可以轻松、快速地进行这些物质的常规分析。收集准确的质量数据将有助于蛋白质和小分子的表征，而对ETD产生的碎片离子的质量分析将使更完整地表征蛋白质翻译后修饰成为可能。新的依赖信息的数据采集程序允许在一次注射过程中完成对多种化合物的分析。使用灵敏的LC/MS/MS和iTRAQ技术的定量方法对一些研究人员来说是必不可少的。该仪器将由蛋白质组学中心训练有素的人员操作和维护，主任将安排日常操作并为用户提供技术专长。一个咨询委员会将确保接触主要用户和其他由小灵通支助的调查人员，并负责该仪器的长期运作。与公共卫生相关的创新生物医学研究需要经常获得最新的分析技术，以增进对人类疾病的了解，并为这些疾病提供新的治疗方法。Thermo Science LTQ Orbitrap混合FT质谱仪和数据系统将允许对许多生物分子进行低水平分析，这些生物分子对石溪NIH支持的研究人员的研究工作至关重要。他们将使用该仪器提供的新的分析技术(精确的质量测量和电子转移解离)来探索导致糖尿病、癌症和心脏病的机制，并帮助为这些疾病状态定义新的生化标记物和药物。

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of a Thermo Scientific LTQ Orbitrap Hybrid FT Mass Spectrometer and Data System. The instrument is linked directly to a Dionex Nano HPLC system affording LC/MS and (MS)n capability and will be located in the Proteomics Center in the Stony Brook School of Medicine. This system will be provide high resolution, accurate mass measurement and electron transfer dissociation, two new mass spectrometer techniques that will significantly promote the research objectives and laboratory productivity of thirteen distinguished scientists from Stony Brook University (SBU) who comprise the major users group. These methods are presently unavailable at SBU and inaccessible or difficult to obtain from other organizations. LC/MS/MS is unsurpassed for the characterization of molecules with a vital biomedical significance, including lipids, biopolymers such as peptides, oligonucleotides, and oligosaccharides and smaller compounds which are non-volatile, labile, or thermally unstable. Techniques for coupling a liquid chromatograph to a mass spectrometer through electrospray or atmospheric pressure chemical ionization sources now permit the routine analysis of these substances with ease and rapidity. Collection of accurate mass data will facilitate protein and small molecule characterization, while the mass analysis of ETD-generated fragment ions will make possible more complete characterization of protein post-translational modifications. New information dependent data acquisition routines permit complete analysis of multiple compounds during a single injection. Quantitative methods employing sensitive LC/MS/MS and iTRAQ techniques are essential for several investigators. The instrument will be operated and maintained by trained personnel at the Proteomics Center, and the Director will schedule daily operation and provide technical expertise for the users. An advisory committee will ensure access to the major users and other PHS-supported investigators and will be responsible for the long term operation of the instrument. PUBLIC HEALTH RELEVANCE Innovative biomedical research requires frequent access to the newest analytical techniques to advance knowledge of human diseases and to provide new treatments for these illnesses. The Thermo Scientific LTQ Orbitrap Hybrid FT Mass Spectrometer and Data System will permit low-level analysis of many biomolecules critical to the research efforts of NIH-supported investigators at Stony Brook. They will use the new, analytical techniques (accurate mass measurement and electron transfer dissociation) offered by this instrument to explore mechanisms leading to diabetes, cancer, and heart disease and help define new biochemical markers and medications for these disease states.