Acquisition of a Thermo Scientific Orbitrap XL Hybrid FT Mass Spectrometer

描述（由申请人提供）：本提案要求为购买Thermo Scientific LTQ Orbitrap混合FT质谱仪和数据系统提供资金。该仪器直接连接到Dionex Nano HPLC系统，提供LC/MS和（MS）n功能，并将位于斯托尼布鲁克医学院的蛋白质组学中心。该系统将提供高分辨率，准确的质量测量和电子转移解离，这两种新的质谱仪技术，将显着促进来自斯托尼布鲁克大学（SBU）的13位杰出科学家的研究目标和实验室生产力，他们包括主要用户组。这些方法目前在SBU是不可用的，也无法从其他组织获得或难以获得。LC/MS/MS在表征具有重要生物医学意义的分子方面是无与伦比的，包括脂质，生物聚合物如肽，寡核苷酸和寡糖以及非挥发性，不稳定或热不稳定的较小化合物。通过电喷雾或大气压化学电离源将液相色谱仪与质谱仪耦合的技术现在允许对这些物质进行简单快速的常规分析。收集准确的质量数据将有助于蛋白质和小分子的表征，而ETD产生的碎片离子的质量分析将使蛋白质翻译后修饰的更完整的表征成为可能。新的信息依赖的数据采集程序允许在一个单一的注射过程中的多种化合物的完整分析。采用灵敏的LC/MS/MS和iTRAQ技术的定量方法对一些研究者来说是必不可少的。该仪器将由蛋白质组学中心训练有素的人员操作和维护，主任将安排日常操作并为用户提供技术专业知识。一个咨询委员会将确保与主要用户和其他公共卫生系统支持的调查人员接触，并将负责该仪器的长期运作。创新的生物医学研究需要经常使用最新的分析技术，以提高对人类疾病的认识，并为这些疾病提供新的治疗方法。Thermo Scientific LTQ轨道阱混合FT质谱仪和数据系统将允许对斯托尼布鲁克的NIH支持的研究人员的研究工作至关重要的许多生物分子进行低水平分析。他们将使用该仪器提供的新的分析技术（精确的质量测量和电子转移解离）来探索导致糖尿病，癌症和心脏病的机制，并帮助定义这些疾病状态的新生化标志物和药物。

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of a Thermo Scientific LTQ Orbitrap Hybrid FT Mass Spectrometer and Data System. The instrument is linked directly to a Dionex Nano HPLC system affording LC/MS and (MS)n capability and will be located in the Proteomics Center in the Stony Brook School of Medicine. This system will be provide high resolution, accurate mass measurement and electron transfer dissociation, two new mass spectrometer techniques that will significantly promote the research objectives and laboratory productivity of thirteen distinguished scientists from Stony Brook University (SBU) who comprise the major users group. These methods are presently unavailable at SBU and inaccessible or difficult to obtain from other organizations. LC/MS/MS is unsurpassed for the characterization of molecules with a vital biomedical significance, including lipids, biopolymers such as peptides, oligonucleotides, and oligosaccharides and smaller compounds which are non-volatile, labile, or thermally unstable. Techniques for coupling a liquid chromatograph to a mass spectrometer through electrospray or atmospheric pressure chemical ionization sources now permit the routine analysis of these substances with ease and rapidity. Collection of accurate mass data will facilitate protein and small molecule characterization, while the mass analysis of ETD-generated fragment ions will make possible more complete characterization of protein post-translational modifications. New information dependent data acquisition routines permit complete analysis of multiple compounds during a single injection. Quantitative methods employing sensitive LC/MS/MS and iTRAQ techniques are essential for several investigators. The instrument will be operated and maintained by trained personnel at the Proteomics Center, and the Director will schedule daily operation and provide technical expertise for the users. An advisory committee will ensure access to the major users and other PHS-supported investigators and will be responsible for the long term operation of the instrument. PUBLIC HEALTH RELEVANCE Innovative biomedical research requires frequent access to the newest analytical techniques to advance knowledge of human diseases and to provide new treatments for these illnesses. The Thermo Scientific LTQ Orbitrap Hybrid FT Mass Spectrometer and Data System will permit low-level analysis of many biomolecules critical to the research efforts of NIH-supported investigators at Stony Brook. They will use the new, analytical techniques (accurate mass measurement and electron transfer dissociation) offered by this instrument to explore mechanisms leading to diabetes, cancer, and heart disease and help define new biochemical markers and medications for these disease states.