Structural Insight for Ca channel Regulation by Calmodulin
Structural Insight for Ca channel Regulation by Calmodulin
批准号:
20770110
负责人:
MORI Masayuki
金额:
$2.83万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2009
中文摘要
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英文摘要
The overall aim of the proposed studies was to understand the mechanism of activation, inhibition and regulations of Ca channels by Calcium-binding protein Calmodulin (CaM). The primary focus was structural determination of the calcium free form apoCaM and Channel IQ domain complex. Until today, we have a couple of crystals, those will be needed to check reproducibility and their structure. For the imaging of Ca^<2+> and CaM- channel interaction, we have succeeded to develop new system combined with Fura-2 and FRET. By using this system we could establish Ca^<2+> titrative experiment in vivo with high fidelity. We hope that this approach will be often to use in the field wide range of the physiological science to reveal Ca^<2+> related molecular dynamics.
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Synergistic activation of vascular TRPC6 channel by receptor and mechanical stimulation via phospholipase C/diacylglycerol and phospholipase A2/omega-hydroxylase/20-HETE pathways
受体和磷脂酶 C/二酰甘油和磷脂酶 A2/omega-羟化酶/20-HETE 途径的机械刺激协同激活血管 TRPC6 通道
DOI:
--
发表时间:
2009
期刊:
Circulation Research 104
影响因子:
--
作者:
[Inoue R, Jensen LJ, Jian Z, Shi J, Hai L, Lurie AI, Henriksen FH, Salomonsson M, Morita H. Kawarabayashi Y, Mori M, Mori Y, Ito Y., 井上隆司 et al.]
通讯作者:
井上隆司 et al.
Structural basis for calmodulin mediated Ca2+ entry channel国際シンポジウム
钙调蛋白介导的Ca2+进入通道的结构基础国际研讨会
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Takahashi S., et.al., 森誠之, 森誠之, 森誠之, 森誠之, 森誠之, 森誠之, 森誠之]
通讯作者:
森誠之
Atomic structure of Ca,2 IQ domain complexed with Ca^<2+>/calmodulin
与Ca^<2>/钙调蛋白复合的Ca,2 IQ结构域的原子结构
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Mori M., et.al.]
通讯作者:
et.al.
Synergistic activation of vascular TRPC6 channel by receptor and mechanical stimulation via phospholipaseC/diacylglycerol and phospholipase A2/omega-hydroxylase/20-HETE pathways
受体和磷脂酶 C/二酰甘油和磷脂酶 A2/omega-羟化酶/20-HETE 途径的机械刺激协同激活血管 TRPC6 通道
DOI:
--
发表时间:
2009
期刊:
Circ Res. 104
影响因子:
--
作者:
[Inoue R, Jensen LJ, Jian Z, Shi J, Hai L, Lurie AI, Henriksen FH, Salomonsson M, Morita H. Kawarabayashi Y, Mori M, Mori Y, Ito Y.]
通讯作者:
Ito Y.
細胞内カルシウムとCa2+チャネル-Calmodulin作用の準同時的イメージング西
细胞内钙和Ca2+通道的准同步成像-钙调蛋白作用
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Takahashi S., et.al., 森誠之]
通讯作者:
森誠之
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Characterization of SAM mice as an ulcerative colitis model
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海外基金