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Isolation of AIDS-related viruses from African Primates

从非洲灵长类动物中分离艾滋病相关病毒

基本信息

批准号：
05041111
负责人：
HAYAMI Masanori
金额：
$ 10.24万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05041111/
关键词：
AIDS Africa HIV Cameroon Double infection recombination SIV 分子疫学ガーナケニアガボンレトロウイルス

项目摘要

1.Since the discovery of HIV-1 and HIV-2 as causative agents of AIDS,efforts have been directed towards understanding the origins and evolutionary history of HIV.HIV-1 and HIV-2 are closely related to primate lentiviruses (SIV). In this project, we have studied the diversity of related nonhuman primate lentiviruses and speculated on the origins of HIV in Africa. In this 3 years, we concentrated to clarify the molecular epidemiology of HIV in Cameroon and to assess the incidence of mixed-infection and recombination.2.Forty eight HIV (47 HIV-1, one HIV-2) were obtained from Cameroonian AC,ARC and AIDS patients in 1994 and 1995. Part of the pol region suitable for comparing all the groups of HIV-related lentiviruses and part of the HIV-1 env region including the V3 loop were amplified by nested PCR,sequenced, and phylogenetically analyzed.3.The majority of Cameroonian HIV-1s belonged to clade A (32), but the rest belonged to various clades : clade B (2), clade C (2), Clade D (2), clade E (1), Clade F (4) and group O (4). In the pol analysis, two different types were found in four individuals (HIV-2a and HIV-1 clade A,HIV-1 group O and clade A,clades A and C,and clades C and F), suggesting double infections with different HIV-1 subtypes or groups.4.Various mixed-infection such as between different clades of HIV-1 group M,between HIV-1 and HIV-2, and even between HIV-1 groups O and M were confirmed at a rather high frequency (around 10%) in the analyzed specimens. Some of the data suggested recombination between different clades of HIV-1 group M were also obtained. Thus phylogenetic analysis based on the pol and env regions provided a new insight to the understanding of HIV phylogeny.

1.自从发现HIV-1和HIV-2是艾滋病的病原体以来，人们一直致力于了解HIV的起源和进化史。HIV-1和HIV-2与灵长类慢病毒（Primate lentiviruses，SIV）密切相关。在这个项目中，我们研究了相关的非人灵长类慢病毒的多样性，并推测了艾滋病毒在非洲的起源。2.1994年和1995年从喀麦隆AC、ARC和AIDS患者中分离到48株HIV，其中47株为HIV-1，1株为HIV-2。通过巢式PCR扩增适于比较所有组的HIV相关慢病毒的pol区的一部分和HIV-1 env区的一部分（包括V3环），测序，并进行遗传学分析。3.大多数爱沙尼亚HIV-1属于进化枝A（32），但其余属于各种进化枝：进化枝B（2）、进化枝C（2）、进化枝D（2）、进化枝E（1）、进化枝F（4）和群O（4）。在pol分析中，4个个体中发现了两种不同的类型（HIV-2a和HIV-1进化枝A、HIV-1 O群和进化枝A、进化枝A和C、进化枝C和F），提示HIV-1不同亚型或群的双重感染。甚至在HIV-1 O和M组之间也以相当高的频率（约10%）在分析的标本中得到确认。一些数据表明，HIV-1 M组不同分支之间的重组也得到了。因此，基于pol和env区域的系统发育分析为理解HIV的遗传学提供了新的视角。