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培養歯根膜細胞移植による人工歯根の歯根膜再生に関する研究

培养牙周膜细胞移植人工牙根牙周膜再生的研究

基本信息

批准号：
05771830
负责人：
小林優
金额：
$ 0.58万
依托单位：
Kanagawa Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至无数据
项目状态：
已结题

项目摘要

人工歯根表面にコラーゲンを固定化し、in vitroで大量に培養した歯根膜細胞を付着させることによって、in vivoにおいて人工歯根周囲に歯根膜の再生を起こしえるかを実験的に検討した。先ず、ヒドロキシアパタイト(HAP)、チタン(Ti)、ポリエチレン(PE)製の3種の人工歯根を作製し、PE製人工歯根の表面にはアテロコラーゲン(豚皮由来、タイプI)を固定化した(CPE)。次に、ビ-グル成犬10頭の臼歯部を抜去し、歯根膜細胞を採取、これまでの研究で明らかにした至適条件(10%FBS+1ng/mlTGF-beta_1添加)で10日間予備培養を行った後、上記の人工歯根を培養液中に7日間浸漬して歯根膜細胞を付着させた。細胞を付着させた人工歯根ならびに対照として細胞を付着させないCPE人工歯根を下顎臼歯部に埋植し、歯肉弁で完全に被覆した。埋植2週および1か月後に屠殺して、非脱灰研磨標本を作製した。その結果、HAPならびにTiでは、埋植2週ですでに活発な骨形成が認められ、埋植1か月では人工歯根表面の大部分が骨と結合し、歯根膜の再生は得られなかった。また、細胞を付着させないCPEは通常の線維性被膜によって被包化されていた。一方、細胞を付着させたCPEでは、人工歯根は骨と一層の軟組織を介し、その線維の走行は材料表面に垂直的で、豊富な血管を含むなど、極めて歯根膜に類似した組織構造が認められた。以上から、材料表面へのコラーゲンの固定化と培養歯根膜細胞の移植は人工歯根の歯根膜再生をもたらす可能性が示された。

On the surface of artificial roots, there are many root membrane cells on the surface of artificial roots, such as immobilization, immobilization and in vitro. The first, the first, the third, the first, the first The root membrane cells of 10-year-old adult dogs were removed, the root membrane cells were removed, and the culture conditions (10%FBS+1ng/mlTGF-beta_1 addition) were studied. After 10 days of incubation, the root membrane cells were immersed in the artificial root culture solution for 7 days. The cells pay for the artificial roots, the artificial roots, the CPE artificial roots, the implanted parts of the mortar, the meat, the covered parts. After 2 months of planting, the standard of non-deashing and grinding will be used as a tool. The results showed that most of the bones were combined on the surface of the artificial roots after 2 months of implantation, and most of the bones were combined on the surface of the artificial roots after 2 months of implantation. The results showed that most of the bones were combined on the surface of the artificial roots, and the root membranes were regenerated. The results showed that most of the bones were combined on the surface of the artificial roots after 2 months of HAP implantation. The cell and the cell pay for the CPE. Usually, the membrane is encapsulated. On the one hand, the cell is responsible for the introduction of the CPE, the artificial bone, the vertical on the surface of the material, the rich blood vessels, and the root membrane. The above, the surface of the material, the immobilization, the root membrane, the cell, the artificial root, the root membrane regeneration, the possibility.