大脳基底核の発達、特に線条体から上丘への一過性線維連絡の解析

基底神经节的发育，特别是从纹状体到上丘的瞬时纤维连接的分析

• 批准号: 05780564
• 负责人: 高田 昌彦
• 金额: $ 0.58万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05780564/
• 关键词: 線条体; 上丘; 細胞死; ドーパミン; 発達; 大脳基底核

1.新生期(生後0、1、2、3、7日)のラットの上丘に逆行性蛍光標識物資(Fluoro-Gold)を注入し、1日の生存期間の後、線条体での標識神経細胞の分布を調べた。生後1、2、3日の線条体では多数の標識細胞がパッチ状に分布していたが、生後4、8日の線条体では標識細胞はみとめられなかった。2.生後0日の上丘にFluoro-Goldを注入したラットを生後4、8日(線条体上丘投射の消失時期)まで生存させた場合、生後4日の線条体ではまだかなりの数の標識細胞がみとめられたが、生後8日の線条体では標識細胞はまったくみとめられなかった。生後0日の線条体に順行性蛍光標識物質(Dil)を注入し、1日の生存期間の後、上丘での標識神経線維の分布を調べた。生後1日の上丘で標識線維がその中間層(第4、5層)にみとめられた。逆行性蛍光標識法とチロシン水酸化酵素を用いた蛍光免疫組織化学とを組み合わせた結果、線条体上丘投射細胞の分布が、線条体のドーパミン神経終末のそれと一致することがわかった。以上の結果から、以下のことが考えられる。1.ラットの脳の発達過程において一過性に出現する線条体上丘投射は生後1週間以内で消失し、その消失機序として起始細胞そのものの死が関与していると考えられる。2.線条体上丘投射細胞は線条体のpatch compartment(ドーパミン神経終末の分布領域)に一致して分布する。

1. Fluoro-Gold was injected into the upper colliculus during neonatal period (0, 1, 2, 3, 7 days after birth), and the distribution of labeled neurons in the postnatal and linear bodies was modulated during the survival period of 1 day. Most of the marker cells were distributed in the striated body on the 1st, 2nd and 3rd day after birth, and the marker cells were distributed in the striated body on the 4th and 8th day after birth. 2. Fluoro-Gold was injected into the upper colliculus on the 0 th day after birth, and on the 4 th and 8 th days after birth (the disappearance period of the upper colliculus projection of the linear body). In addition, the number of marker cells in the linear body was reduced on the 4 th day after birth, and the number of marker cells in the linear body was reduced on the 8 th day after birth. After 0 days of life, the anterograde fluorescent marker substance (Dil) was injected into the line body, and after 1 day of life, the distribution of the marker line dimension in the upper mound was modulated. On the 1st day after birth, the upper mound marks the middle layer (the 4th and 5th layers). The results of retrograde light labeling, immunohistochemistry and immunohistochemistry were compared. The distribution of projection cells in the upper colliculus of the striated body was consistent with that of the terminal of the striated body. The above results are different from the following results. 1. During the development process of the cell, the linear projection disappeared within one week after birth, and the disappearance mechanism of the initial cell was related to the death of the cell. 2. The projection cells of the striated supracolliculus are uniformly distributed in the patch compartment of the striated body.

项目成果

Kondo,Y.,Takada,M.,Tokuno,H.& Mizuno,N.: "Single retinal ganglion cells projecting bilaterally to the lateral geniculate nuclei or superior colliculus by way pf axon.." Journal of Comparative Neurology. (in press).

近藤，Y.，高田，M.，德野，H.

Tahada,M., Tokuno,H.,Ikai,Y.& Mizuno,N.: "Direct projections from the entopeduncular nucleus to the lower brainstem in the rat" Journal of Comparative Neurology. (in press).

多哈达，M.，德野，H.，Ikai，Y.

Shinonaga,Y.,Takada,M.& Mizuno,N.: "Topographic oranization of collateral projections from the the basolateral amygdaloid nucleus to both the prefrontal cortex and...." Neuroscience. 58. 389-397 (1994)

筱长，Y.，高田，M.

Shinonaga,Y.,Takada,M.& Mizuno,N.: "Direct projections from the non-laminated divisions of the medial genicu late nucleus to the temporal polar cortex and amygdala...." Journal of Comparative Neurology. 340. 405-426 (1994)

筱长，Y.，高田，M.

Tokuno,H.,Takada,M.,Ikai,Y.& Mizuno,N.: "Direct projections from the deep layers of the superior colliculusto the subthalmic nucleus in the rat" Brain Research. (in press).

德野，H.，高田，M.，伊凯，Y.