Joint study on oral asaccharolytic Eubacterium species.

口腔解糖真杆菌属的联合研究。

• 批准号: 06044082
• 负责人: HOSHINO Etsuro
• 金额: $ 3.97万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044082/
• 关键词: Eubacterium; asaccharolytic; oral cavity; obligate anaerobes; taxonomy; new species

Asaccharolytic Eubacterium species are strictly anaerobic, gram-positive rods and are frequently isolated and predominant bacterial species from different oral samples. The slow growth and oxygen sensitivity of the oral asaccharolytic Eubacterium species has hindered the determination of their significance in various oral infectious diseases. By adopting efficient anaerobic isolation procedures, we isolated several unclassified strains of Eubacterium that were distinct from the established Eubacterium species namely, E.brachy, E.lentum, E.nodatum and E.timidum. A polyphasic approach was used to examine the taxonomic positions of these organisms. These un-named strains were inert in conventional biochemical tests, however, the results of SDS-PAGE protein profile analysis and aminopeptidase activty (Rapid ID 32A) revealed that these strains were quite distinct compared from the established asaccharolytic species and assigned to four groups. Two groups of strains, is butyrate producing gram-positive short rods, have been proposed new species namely, E.saphenum and E.minutum, established on the additional basis of western immunoblotting reactions and DNA-DNA hybridization analysis.The third group were phenylacetate producing strains, which can be distinguished from E.timidum by the differences in whole-cell protein profile and aminopeptidase activity. The strains in the fourth group produced a trace (if any) of phenylacetate. The continuos development and improvement of anaerobic incubation and identification techniques resulted in the isolation of slow growing and culture-difficult asaccharolytic species of Eubacterium. Further studies are in progress to provide information on the roles of the unclassified strains and other Eubacterium species in oral disease.

溶天冬氨酸真杆菌属是严格厌氧的革兰氏阳性杆菌，经常从不同的口腔样本中分离出来，并且是主要的细菌物种。口腔溶砷真杆菌的缓慢生长和氧敏感性阻碍了它们在各种口腔感染性疾病中的意义的确定。通过采用有效的厌氧分离程序，我们分离了几种未分类的真杆菌属菌株，这些菌株不同于已建立的真杆菌属物种，即短真杆菌、迟缓真杆菌、结节真杆菌和侏儒真杆菌。一个多相的方法被用来检查这些生物体的分类位置。这些未命名的菌株在常规生化试验中呈惰性，但SDS-PAGE蛋白质图谱分析和氨基肽酶活性（Rapid ID 32A）测定结果表明，这些菌株与已建立的溶天冬氨酸菌株有很大区别，并可分为4个类群。通过Western免疫印迹和DNA-DNA杂交分析，确定了两组产丁酸的革兰氏阳性短杆菌菌株为新种，即隐球菌（E.saphenum）和微小杆菌（E.minutum）;第三组产苯乙酸的菌株可通过全细胞蛋白质谱和氨肽酶活性的差异与短杆菌（E.timidum）区分。第四组中的菌株产生痕量（如果有的话）苯乙酸盐。随着厌氧培养和鉴定技术的不断发展和完善，分离到了生长缓慢、培养困难的厌氧分解真杆菌。进一步的研究正在进行中，以提供有关未分类菌株和其他真杆菌属物种在口腔疾病中的作用的信息。

项目成果

M.A.Hamid: "The metabolism of phenylalanine and leucine by a cell suspension of Eubacterium brachy and the effects of metronidazole on metabolism." Archives of Oral Biology. 39. 967-972 (1994)

M.A.Hamid：“真杆菌细胞悬液对苯丙氨酸和亮氨酸的代谢以及甲硝唑对代谢的影响。”

Nakazawa F and Hoshino E: "Genetic relationships among Eubacterium species." International Journal of Systematic Bacteriology. 44. 787-790 (1994)

Nakazawa F 和 Hoshino E：“真杆菌物种之间的遗传关系。”

Sato T, Uematsu H, Sato M, Nakazawa F and Hoshino E.: Science Review. Eubacterium saphenum isolated from human periodontal pockets. In : Medical and Dental Aspects of Anaerobes, 33-37 (1995)

佐藤 T、植松 H、佐藤 M、中泽 F 和星野 E.：科学评论。

Cheeseman SL, Weightman AJ and Wade WG.: Science Review. 16S rRNA sequence comparisons of oral asaccharolytic Eubacterium species. In : Medical and Dental Aspects of Anaerobes, 39-43 (1995)

Cheeseman SL、Weightman AJ 和 Wade WG.：《科学评论》。

Takuichi Sato: "Predominant obligate anaerobes in necrotic pulps of human deciduous teeth." Microbial Ecology in Health and Disease. 6. 269-275 (1993)

Takuichi Sato：“人类乳牙坏死牙髓中主要是专性厌氧菌。”