Joint study on oral asaccharolytic Eubacterium species.

口腔解糖真杆菌属的联合研究。

基本信息

批准号：
06044082
负责人：
HOSHINO Etsuro
金额：
$ 3.97万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044082/
关键词：
Eubacterium asaccharolytic oral cavity obligate anaerobes taxonomy new species

项目摘要

Asaccharolytic Eubacterium species are strictly anaerobic, gram-positive rods and are frequently isolated and predominant bacterial species from different oral samples. The slow growth and oxygen sensitivity of the oral asaccharolytic Eubacterium species has hindered the determination of their significance in various oral infectious diseases. By adopting efficient anaerobic isolation procedures, we isolated several unclassified strains of Eubacterium that were distinct from the established Eubacterium species namely, E.brachy, E.lentum, E.nodatum and E.timidum. A polyphasic approach was used to examine the taxonomic positions of these organisms. These un-named strains were inert in conventional biochemical tests, however, the results of SDS-PAGE protein profile analysis and aminopeptidase activty (Rapid ID 32A) revealed that these strains were quite distinct compared from the established asaccharolytic species and assigned to four groups. Two groups of strains, is butyrate producing gram-positive short rods, have been proposed new species namely, E.saphenum and E.minutum, established on the additional basis of western immunoblotting reactions and DNA-DNA hybridization analysis.The third group were phenylacetate producing strains, which can be distinguished from E.timidum by the differences in whole-cell protein profile and aminopeptidase activity. The strains in the fourth group produced a trace (if any) of phenylacetate. The continuos development and improvement of anaerobic incubation and identification techniques resulted in the isolation of slow growing and culture-difficult asaccharolytic species of Eubacterium. Further studies are in progress to provide information on the roles of the unclassified strains and other Eubacterium species in oral disease.

糖类聚细菌种类严格是厌氧，革兰氏阳性的棒，经常与不同的口服样品分离出来，主要是细菌。口服散酸酸细菌的生长缓慢和氧气敏感性阻碍了它们在各种口腔传染病中的重要性。通过采用有效的厌氧分离程序，我们分离了几个未分类的菌群菌株，这些菌株与已建立的卵细菌种不同，即E.brachy，E.Lentum，E.Lentum，E.Nodatum和E.timidum。采用多方方法来检查这些生物的分类立场。这些未命名的菌株在常规的生化测试中是惰性的，但是，SDS-PAGE蛋白谱分析和氨基肽酶活性的结果（Rapid ID 32A）表明，这些菌株与已建立的糖糖溶解物种相比完全不同，并分配给四组。 Two groups of strains, is butyrate producing gram-positive short rods, have been proposed new species namely, E.saphenum and E.minutum, established on the additional basis of western immunoblotting reactions and DNA-DNA hybridization analysis.The third group were phenylacetate producing strains, which can be distinguished from E.timidum by the differences in whole-cell protein profile and aminopeptidase activity.第四组中的菌株产生了痕量（如果有的话）。厌氧孵化和鉴定技术的连续性发展和改善导致分离生长缓慢和培养细菌的炎多糖类。进一步的研究正在进行中，以提供有关未分类菌株和其他卵细菌在口腔疾病中的作用的信息。