Capsulation of infected bacterial masses as a new immunological reaction

感染细菌团的包裹作为一种新的免疫反应

• 批准号: 12470383
• 负责人: HOSHINO Etsuro
• 金额: $ 4.1万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470383/
• 关键词: immune system; biofilm; actinomycosis; capusulation; neutorophils; 国際研究者交流; 多国籍; Actinomyces israelii; Actinomycosis; 被膜; Actinomyces; 弱抗酸性菌; 根尖病巣

The present research aimed to clarify the association of capsulation of bacterial masses with bacterial biofilm formation in actinomycotic and similar lesions, as a new host immune reaction.1. Substances of capsules : Eosinophilic amorphous plastic-like structure surrounding bacteria masses in actinomycosis lesions was found to contained DNA and proteineous contents of neutrophils using fluorescent labeling techniques in vitro. Such bacteria in lesions were often stained to be weak acid-fast, depending on the amount of capsular substances. The bacterial masses in actinomycotic lesions are, thus, separated and isolated by the capsulation, which may reveal the characteristics of actinomycotic symptoms.2. Capsulation in periapical lesions : Weekly acid-fast bacteria, including Actinomyces israelii and the similar types of bacteria such as Asaccharolytic Anaerobic Gram-positive Rods : AAGPR, were often found in periapical lesions collected in Asian countries including Japan. Tight biofilm capsulation may cause worse prognosis in endodontic treatment.3. 3Mix-MP to treat the lesions with capsulated bacteria masses. A combination of 3 anti-bacteria drugs (3Mix) is efficient against all the types of oral bacteria. Propylene glycol (P in MP) makes it possible to carry 3Mix through the capsules efficiently and kill bacteria inside.

本研究旨在阐明放线菌病和类似病变中细菌生物膜形成与细菌包被的关系，作为一种新的宿主免疫反应。胶囊物质：应用荧光标记技术，发现放线菌病皮损中细菌周围的嗜酸性无定形塑料样结构含有中性粒细胞的DNA和蛋白质。病变中的此类细菌通常被染色为弱耐酸，这取决于荚膜物质的量。因此，放线菌病病斑中的菌群被包囊分离和隔离，这可能揭示放线菌病的特征.根尖周病变中的包囊：在亚洲国家（包括日本）收集的根尖周病变中经常发现每周抗酸细菌，包括以色列放线菌和类似类型的细菌，如抗坏血酸厌氧革兰氏阳性杆菌（AAGPR）。根管治疗中，生物膜包裹过密可能导致预后不良. 3混合MP治疗有包膜菌团的病变。3种抗菌药物（3Mix）的组合对所有类型的口腔细菌都有效。丙二醇（MP中的P）可以有效地携带3Mix通过胶囊并杀死里面的细菌。

项目成果

Hashimura T.: "Detection of Slackia exigua, Mogibacterium timidum and Eubacterium saphenum from pulpal and periradicular samples using the PCR method"International Endodontic Journal. 34. 463-470 (2001)

Hashimura T.：“使用 PCR 方法从牙髓和根尖周样本中检测甜菜松弛菌、Timidum 莫吉杆菌和隐真杆菌”《国际牙髓杂志》。

Hashimura, T.: "Detection of Slackia exigua, Mogibacterium timidum and Eubacterium saphenum from pulpal and periradicular samples using the Polymerase Chain Reaction (PCR) method (PCR) method"International Endodontic Journal. 34・6. 463-470 (2001)

Hashimura, T.：“使用聚合酶链式反应（PCR）方法（PCR）从牙髓和根尖周样本中检测甜菜芽孢杆菌、Mogibacter timidum 和真杆菌”国际牙髓杂志 34・6（2001）。

Nakazawa F.: "Taxonomic characterization of Mogibacterium diversum sp. nov. and Mogibacterium neglectum sp. nov., isolated from oral cavities"International Journal of Systematic and Evolutionary Microbioloy. 52. 115-122 (2002)

Nakazawa F.：“从口腔中分离出的 Mogibacter diversum sp. nov. 和 Mogibacterignesum sp. nov. 的分类学特征”《国际系统与进化微生物学杂志》。

MD.A.Salam: "Intraperitoneal immune cell responses by Eubacterium saphenum in mice"Microbiology and Immunology. 45(1). 29-37 (2001)

MD.A.Salam：“小鼠隐真杆菌的腹膜内免疫细胞反应”微生物学和免疫学。

E.V.Cruz: "Penetration of propylene glycol through dentine"International Endodontic Journal. 35. 330-336 (2002)

E.V.Cruz：“丙二醇通过牙本质的渗透”国际牙髓杂志。