Capsulation of infected bacterial masses as a new immunological reaction

本研究旨在阐明放线菌病及类似病变中细菌团块被包膜与细菌生物膜形成的关系，作为一种新的宿主免疫反应。胶囊物质：体外荧光标记技术发现放线菌病病变菌团周围的嗜酸性无定形塑料样结构含有中性粒细胞的DNA和蛋白质含量。病变中的这种细菌通常被染色为弱抗酸，这取决于荚膜物质的数量。因此，在放线菌病的病变中，细菌团被胶囊分离和隔离，这可能揭示放线菌病的症状特征。根尖周病变被包膜：在日本等亚洲国家收集的根尖周病变中，经常发现周抗酸菌，包括以色列放线菌和类似类型的细菌，如无糖解氧革兰氏阳性棒：AAGPR。致密的生物膜包膜可能导致牙髓治疗预后较差。3Mix-MP用于治疗有包膜菌团的病变。3种抗菌药物（3Mix）的组合对所有类型的口腔细菌都有效。丙二醇（MP中的P）使3Mix能够有效地通过胶囊并杀死里面的细菌。

The present research aimed to clarify the association of capsulation of bacterial masses with bacterial biofilm formation in actinomycotic and similar lesions, as a new host immune reaction.1. Substances of capsules : Eosinophilic amorphous plastic-like structure surrounding bacteria masses in actinomycosis lesions was found to contained DNA and proteineous contents of neutrophils using fluorescent labeling techniques in vitro. Such bacteria in lesions were often stained to be weak acid-fast, depending on the amount of capsular substances. The bacterial masses in actinomycotic lesions are, thus, separated and isolated by the capsulation, which may reveal the characteristics of actinomycotic symptoms.2. Capsulation in periapical lesions : Weekly acid-fast bacteria, including Actinomyces israelii and the similar types of bacteria such as Asaccharolytic Anaerobic Gram-positive Rods : AAGPR, were often found in periapical lesions collected in Asian countries including Japan. Tight biofilm capsulation may cause worse prognosis in endodontic treatment.3. 3Mix-MP to treat the lesions with capsulated bacteria masses. A combination of 3 anti-bacteria drugs (3Mix) is efficient against all the types of oral bacteria. Propylene glycol (P in MP) makes it possible to carry 3Mix through the capsules efficiently and kill bacteria inside.