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Predominant culture-difficult bacteria in periodontal pockets and their pathogenicity.

牙周袋中的优势培养困难菌及其致病性。

基本信息

批准号：
09470390
负责人：
HOSHINO Etsuro
金额：
$ 3.39万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470390/
关键词：
culture-difficult bacteria periodonttitis asaccharolytic bacteria oral anaerobes γδ T cell IL-4 IFNィイD2γィエD2

项目摘要

The present project aimed to clarify the pathogenic factors of asaccharolytic anaerobic Gram-positive rods (AAGPR). AAGPR are so culture-difficult that they had been ignored until we reported they were predominant in various oral florae when excellent strict anaerobic procedures were adapted. Most of them are not classified to any established bacterial genera and species, thus, another aim is to clarified their phylogenetic positions.1. Groups of clinical isolates were assigned to 2 new bacterial genera, Cryptobacterium and Mogibacterium, and 4 new species according to the 16SrDNA sequence analysis results.2. PCR method may be useful for the detection of culture-difficult bacteria, so that species-specific primers of AAGPR were designated, and in fact, they were detected from various oral sites.3. When AAGPR were injected into mouse peritoneal cavities, γδ_ cell responded primary to produce IL-4 and IFNィイD2γィエD2, indicating they induced humoral and cellular immunities. The immune reactions were as strong as those induced by Porphyromonas gingivalis injected intraperioneally as a control bacteria, suggesting AAGPR possess high immunological potency and play an important role in periodontitis as predominant bacteria in periodontal pockets.

本项目旨在阐明无糖解氧革兰氏阳性棒（AAGPR）的致病因素。AAGPR是如此难以培养，他们一直被忽视，直到我们报道他们在各种口腔菌群中占主导地位，当优秀的严格的厌氧程序适应。它们中的大多数没有被分类到任何已确定的细菌属和种，因此，另一个目的是澄清它们的系统发育位置。根据16SrDNA序列分析结果，将临床分离菌株归为隐菌（Cryptobacterium）和莫吉杆菌（Mogibacterium） 2个新属和4个新种。PCR方法可用于检测培养困难的细菌，因此指定了AAGPR的种特异性引物，事实上，在口腔的各个部位都检测到了AAGPR引物。将AAGPR注入小鼠腹膜腔后，小鼠的γδ_细胞产生IL-4和IFN γ γ γ γ γ γ γ γ γ γ D2，表明它们诱导了体液免疫和细胞免疫。免疫反应与对照菌龈卟啉单胞菌腹腔注射引起的免疫反应相同，提示AAGPR作为牙周袋内优势菌具有较高的免疫效力，在牙周炎中发挥重要作用。