Predominant culture-difficult bacteria in periodontal pockets and their pathogenicity.

牙周袋中的优势培养困难菌及其致病性。

• 批准号: 09470390
• 负责人: HOSHINO Etsuro
• 金额: $ 3.39万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470390/
• 关键词: culture-difficult bacteria; periodonttitis; asaccharolytic bacteria; oral anaerobes; γδ T cell; IL-4; IFNィイD2γィエD2

The present project aimed to clarify the pathogenic factors of asaccharolytic anaerobic Gram-positive rods (AAGPR). AAGPR are so culture-difficult that they had been ignored until we reported they were predominant in various oral florae when excellent strict anaerobic procedures were adapted. Most of them are not classified to any established bacterial genera and species, thus, another aim is to clarified their phylogenetic positions.1. Groups of clinical isolates were assigned to 2 new bacterial genera, Cryptobacterium and Mogibacterium, and 4 new species according to the 16SrDNA sequence analysis results.2. PCR method may be useful for the detection of culture-difficult bacteria, so that species-specific primers of AAGPR were designated, and in fact, they were detected from various oral sites.3. When AAGPR were injected into mouse peritoneal cavities, γδ_ cell responded primary to produce IL-4 and IFNィイD2γィエD2, indicating they induced humoral and cellular immunities. The immune reactions were as strong as those induced by Porphyromonas gingivalis injected intraperioneally as a control bacteria, suggesting AAGPR possess high immunological potency and play an important role in periodontitis as predominant bacteria in periodontal pockets.

目前的项目旨在阐明散热性厌氧革兰氏阳性杆（AAGPR）的致病因素。 AAGPR是如此文化困难，以至于在我们报告对极好的严格厌氧手术进行改编时，它们在各种口腔植物中占主导地位。他们中的大多数人都不分为任何已建立的细菌属和物种，因此，另一个目的是阐明其系统发育位置。1。根据16srDNA序列分析结果，将临床分离株组分配给2个新细菌属，隐杆菌和摩根杆菌和4种新物种。2。 PCR方法可能有助于检测培养细菌，因此指定了AAGPR的物种特异性引物，实际上，它们是从各个口服位点检测到的。3。当AAGPR注入小鼠腹膜腔时，γδ_细胞反应起来产生IL-4和IFNII D2，表明它们诱导了体液和细胞免疫。免疫反应与牙龈牙龈毒死症诱导的免疫反应一样强，作为对照细菌注射，表明AAGPR具有很高的免疫学效能，并在牙周炎中起着重要作用，作为牙周口袋中的主要细菌。

项目成果

Umemoto T: "Treponema medium sp. nov., isolated from human subgingival dental plaque."International Journal of Systematic 【approximately equal】 Bacteriology. 47. 67-72 (1997)

Umemoto T：“Treponema media sp. nov.，从人类龈下牙菌斑中分离出来。”《国际系统学杂志》[大约相等]细菌学 47. 67-72 (1997)。

MD. ali Asgor Moral: "Experimantal chronic infection induced in mice by Actinomyces israelii entrapped by alginate gel."Archives of oral biology. 43. 485-496 (1998)

医学博士。

Sato T: "Restricted fragment-length polymorphism analysis of 16SrDNA from oral asaccharolytic Eubacterium species amplified by polymerase chain reaction"Oral Microbiology and Immunology. 13. 23-29 (1998)

Sato T：“通过聚合酶链反应扩增的口腔解糖真杆菌种的 16SrDNA 的限制片段长度多态性分析”口腔微生物学和免疫学。

Nakazawa F: "Cryptobacterium curtum gen. nov., sp. nov., a new of Gram-positive anaerobic rod isolated from human oral cavities."International Journal of Systematic genus Bacteriology. 49(3). 1193-1200 (1999)

Nakazawa F：“Cryptobacter curtum gen. nov.，sp. nov.，从人类口腔中分离出的一种新的革兰氏阳性厌氧杆菌。”国际系统属细菌学杂志。

Sumita M: "Experimental actinomycosis in mice using alginate gel particles containing Actinomyces israelli"Endodontics and Dental Traumatology. 14. 137-143 (1998)

Sumita M：“使用含有以色列放线菌的藻酸盐凝胶颗粒对小鼠进行实验性放线菌病”牙髓学和牙科创伤学。