Magnetic bead-antibody complex and the application for oral infectious diseases.

本研究的目的是利用免疫磁珠（immunomagnetic beads，IMB）包被特异性病原菌抗体，建立去除口腔感染性病变中特异性病原菌的系统，制备的抗体-IMB复合物与靶菌发生特异性反应，牙龈卟啉单胞菌被认为是牙周病相关的细菌。因此，该复合物可以捕获范围为7 X IO2至IO7的牙龈卟啉单胞菌细胞。此外，特异性IMB可以从其他种类的细菌混合物中选择性地捕获目标细菌细胞，包括口腔微生物区系中的各种细菌。捕获的细菌将被磁棒去除并从细菌悬浮液中删除。每种删除程序的效力范围为29（] SY ± 1）。[)3至42（] SY. ±. [)1%.当磁珠结合的细菌暴露于微波照射后的免疫磁性分离，特异性IMB被发现加速细菌的杀伤。本研究表明，IMB系统是一种简单、快速的选择性分离和删除细菌的技术。这种方法可用于从口腔感染部位（如牙周袋）删除某些目标细菌。

The aim of this study is establish the system to delete or remove the specific bacteria from oral infectious lesions using immunomagnetic beads (IMB) coated with anti-bodies against specific pathogenic bacteria.The prepared antibody-IMB complex reacted specifically with the target bacteria, e.g., Porphyromonas gingivalis which is suggested as periodontal disease associated bacterial species. Thus, the complex could capture P.gingivalis cells ranging from 7 x102 to 107. Moreover, the specific IMB could selectively capture the target bacterial cells from mixture of other kinds of bacteria including various kinds of bacteria in oral microflora. The captured bacteria would be removed by magnetic bars and deleted from bacterial suspension. The efficacy of each deletion procedure ranged from 29(]SY.+-。[)3 to 42(]SY.+-。[)1%. When bead-bound bacteria were exposed to microwave irradiation after the immunomagnetic separation, specific IMB was found to accelerate the bacterial killing. The present study demonstrates that the IMB system is a simple and rapid technique for selective separation and deletion of bacteria. This approach is useful to delete certain target bacteria from oral infectious sites like periodontal pockets.