Magnetic bead-antibody complex and the application for oral infectious diseases.

磁珠-抗体复合物及其在口腔感染性疾病中的应用。

• 批准号: 07557113
• 负责人: HOSHINO Etsuro
• 金额: $ 4.74万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557113/
• 关键词: immunomagnetic beads; magnetic deletion; Porphyromonas gingivalis; 歯周疾患; 除菌・殺菌

The aim of this study is establish the system to delete or remove the specific bacteria from oral infectious lesions using immunomagnetic beads (IMB) coated with anti-bodies against specific pathogenic bacteria.The prepared antibody-IMB complex reacted specifically with the target bacteria, e.g., Porphyromonas gingivalis which is suggested as periodontal disease associated bacterial species. Thus, the complex could capture P.gingivalis cells ranging from 7 x102 to 107. Moreover, the specific IMB could selectively capture the target bacterial cells from mixture of other kinds of bacteria including various kinds of bacteria in oral microflora. The captured bacteria would be removed by magnetic bars and deleted from bacterial suspension. The efficacy of each deletion procedure ranged from 29(]SY.+-。[)3 to 42(]SY.+-。[)1%. When bead-bound bacteria were exposed to microwave irradiation after the immunomagnetic separation, specific IMB was found to accelerate the bacterial killing. The present study demonstrates that the IMB system is a simple and rapid technique for selective separation and deletion of bacteria. This approach is useful to delete certain target bacteria from oral infectious sites like periodontal pockets.

这项研究的目的是建立了使用免疫磁性珠（IMB）从口腔传染水平中删除或去除特定细菌的系统，该系统与特定的致病细菌涂有抗体涂有抗体。制备的抗体-IMB复合物与靶细菌，例如，孢子虫，牙龈g虫相关的细菌，与周期性疾病相关。这是该复合物可以捕获7 x102至107的吉维利斯细胞。此外，特定的IMB可以从其他类型的细菌的混合物中有选择地捕获靶细菌细胞，包括口腔微生物中的各种细菌。捕获的细菌将通过磁条去除并从细菌悬浮液中删除。每个删除过程的效率范围为29（]SY。+ - 。[）3至42（]SY。+ - 。[）1％。当免疫磁分离后，将珠子结合的细菌暴露于微波辐射时，发现特异性IMB会加速杀死细菌。本研究表明，IMB系统是一种简单而快速的技术，用于选择性分离和缺失细菌。这种方法可用于从口腔传染性部位（如牙周口袋）中删除某些靶标细菌。