Magnetic bead-antibody complex and the application for oral infectious diseases.

本研究的目的是利用免疫磁珠（IMB）包被针对特定致病菌的抗体，建立一种清除口腔感染性病变中特定细菌的系统。制备的抗体- imb复合物与目标细菌特异性反应，例如牙龈卟啉单胞菌，这被认为是牙周病相关的细菌种类。因此，该复合物可以捕获7 × 102到107的牙龈假单胞菌细胞。此外，特异性IMB可以选择性地从口腔菌群中多种细菌的混合物中捕获目标细菌细胞。捕获的细菌将被磁棒去除，并从细菌悬浮液中删除。每个删除程序的有效性从29(]SY.+-。[)3 ~ 42(]SY.+- .[)1%。将免疫磁分离后的细菌置于微波辐照下，发现特异性IMB能加速细菌的杀灭。本研究表明，IMB系统是一种简单、快速的细菌选择性分离和删除技术。这种方法有助于从口腔感染部位（如牙周袋）中清除某些目标细菌。

The aim of this study is establish the system to delete or remove the specific bacteria from oral infectious lesions using immunomagnetic beads (IMB) coated with anti-bodies against specific pathogenic bacteria.The prepared antibody-IMB complex reacted specifically with the target bacteria, e.g., Porphyromonas gingivalis which is suggested as periodontal disease associated bacterial species. Thus, the complex could capture P.gingivalis cells ranging from 7 x102 to 107. Moreover, the specific IMB could selectively capture the target bacterial cells from mixture of other kinds of bacteria including various kinds of bacteria in oral microflora. The captured bacteria would be removed by magnetic bars and deleted from bacterial suspension. The efficacy of each deletion procedure ranged from 29(]SY.+-。[)3 to 42(]SY.+-。[)1%. When bead-bound bacteria were exposed to microwave irradiation after the immunomagnetic separation, specific IMB was found to accelerate the bacterial killing. The present study demonstrates that the IMB system is a simple and rapid technique for selective separation and deletion of bacteria. This approach is useful to delete certain target bacteria from oral infectious sites like periodontal pockets.