Development of ecological bioprocesses for the production of heat shock proteins using aqueous two-phase systems

使用水性两相系统生产热休克蛋白的生态生物工艺的开发

• 批准号: 06555237
• 负责人: KOMASAWA Isao
• 金额: $ 3.2万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06555237/
• 关键词: Aqueous two-phase; Heat shock protein; Bioprocess; E.coli; GroEL; GroES; CAB; Structure formation

The effective refolding process was designed and proposed as the extractive refolding process using aqueous two-phase systems (ATPS) mediated with heat shock proteins (HSP) at higher temperature.In this integrated extractive refolding process which combined and exploited both functions of heat shock proteins and ATPS,the refolding rate can be accelerated suggesting the advantage of refolding at high temperature. Furthermore the activity obtained from more hydrophilic phase can be improved due to prevention of aggregation and to protection from heat inactivation of GroEL and refolded protein can be concentrated into the hydrophilic phase separating from the denatured protein.

设计并提出了一种高效的复性工艺-热休克蛋白（HSP）介导的双水相体系（ATPS）高温萃取复性工艺，该工艺充分利用了热休克蛋白和ATPS的双重功能，加快了复性速度，体现了高温复性的优势。此外，由于防止聚集和保护免于GroEL的热失活，可以提高从更亲水相获得的活性，并且重折叠的蛋白质可以浓缩到与变性蛋白质分离的亲水相中。

项目成果

Koji Yano: "Studies on Retalding with Host Shock Proteins Basecl on Their Partitioning Behabior in Aqueous Two-Phase systems" Proc.I.S.E.C.'96. (in press). (1996)

Koji Yano：“基于宿主休克蛋白在水性两相系统中的分配行为的 Retalding 研究”Proc.I.S.E.C.96。

Ryoichi Kuboi: "Puritication Process for Heat Shock Proteins Using Agueous Two-Phase system and PEG Fractional Precipitation" J.Chem.Eng.Japan. 28. 797-802 (1995)

Ryoichi Kuboi：“使用水两相系统和 PEG 分级沉淀的热休克蛋白纯化工艺”J.Chem.Eng.Japan。

K.Yano, K.Minagawa, R.Kuboi and I.Komasawa: "Partitioning Behaviors of Stressed Proteins in Aqueous Two-Phase Systems" Biosep.ATPS. (in press).

K.Yano、K.Minakawa、R.Kuboi 和 I.Komasawa：“水性两相系统中应激蛋白的分配行为”Biosep.ATPS。

K.Yano, T.Hasegawa, R.Kuboi, I.Komasawa: "Studies on Refolding with Heat Shock Proteins Based on Their Partitioning Behavior in Aqueous Two-Phase systems" Proc.ISEC'96. (in press).

K.Yano、T.Hasekawa、R.Kuboi、I.Komasawa：“基于热休克蛋白在水性两相系统中的分配行为的研究”Proc.ISEC96。

Ryoichi Kuboi: "Purification Process for Heat Shock Proteins Using Aqueous Two-Phase System and PEG Fractional Precipitation" J.Chem.Eng.Japan. 28. 797-802 (1995)

Ryoichi Kuboi：“使用水性两相系统和 PEG 分级沉淀的热激蛋白纯化工艺”J.Chem.Eng.Japan。