Pathology and Molecular Evolution of HTLV in Asia

亚洲 HTLV 的病理学和分子进化

• 批准号: 07042008
• 负责人: OSAME Mitsuhiro
• 金额: $ 10.24万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07042008/
• 关键词: HTLV; ATL; molecular epidemiology; phylogenetic analysis; STLV; HAM; TSP; HTLV-I; STLV-I; 少数民族; 分子系統解析; ヒト細胞白血病; HTLV-1; STLV-1; オランウータン; ピグミー

The purpose of this resesrch is to clarify the HTLV/STLV infection in Asia and we conducted the molecular epidemiological search and analyzed pathogenic relations of HLA.1) STLV-I positive cases were newly detected on 3 orangutans and 1 gibbon in Indonesia. Genetic analysis showed STLV-I of orangutan was quite variable and made two clusters in phylogenetictree. It is not likely that Melanesian group of HTLV-I directly came from orangutan although the group belongs to big Asian STLV-I group. 2) Seroscreening for HTLV-I was carried out in India (400 blood donors in Kelara, 20 HAM/TSP patients in karnataka), Malaysia (50 donors in Kelantan, Northern Malaysia) , Thailand (20 T-lymphoma patients in Songkhula, Southern Thailand) and Indonesia (220 Iryanese, Inryan Jaya). HTLV was not endemic among none of the ethnic groups tested. 3) In order to study molecular phylogenetic relationships among the HTLV isolates, genomic DNAs were extracted from lymphocytes of seropositive subjects in India, Andra Pradesh, and used as templates in PCR studies. provirus genome was detected in two independent families in Andra Pradesh. The lymphocytes were cultured in the presence of IL-2. After six months in culture, cells expressing HTLV specific antigens showed proliferation. 4) About 200 samples from Pakistan and Afghanistan including minority of mountain area, about 900 samples from Azerbaijan, and about 900 samples from Altai were screened and resulted in all negative for HTLV-I.5) HLA typing of HAM/TSP patients from Mashhadi Jews showed high prevalence of B52 type, supporting the important relations of the tupe to HTLV-I pathogenesis.

本研究的目的是阐明亚洲HTLV/STLV感染的分子流行病学研究，并分析其与HLA的致病关系。1）在印度尼西亚新发现的3只猩猩和1只长颈鹿的STLV-I阳性病例。遗传分析表明，猩猩STLV-I变异较大，在遗传树中形成两个聚类。HTLV-I的美拉尼西亚群不太可能直接来自猩猩，尽管该群属于亚洲的大STLV-I群。2)在印度（Kelara的400名献血者，卡纳塔克邦的20名HAM/TSP患者）、马来西亚（北方马来西亚的吉兰丹的50名献血者）、泰国（泰国南部Songkhula的20名T淋巴瘤患者）和印度尼西亚（Inryan Jaya的220名Iryanese患者）进行了HTLV-I的血清筛查。HTLV不是地方性的，没有测试的种族群体。3)为了研究HTLV分离株之间的分子系统发育关系，从印度Andra Pradesh的血清阳性受试者的淋巴细胞中提取基因组DNA，并用作PCR研究中的模板。在安得拉邦的两个独立的家庭中检测到前病毒基因组。在IL-2存在下培养淋巴细胞。培养6个月后，表达HTLV特异性抗原的细胞显示增殖。4)对来自巴基斯坦和阿富汗的200份标本（包括山区少数民族）、阿塞拜疆的900份标本和阿尔泰的900份标本进行了HTLV-I筛查，结果均为阴性。5）Mashhadi犹太人HAM/TSP患者的HLA分型显示B52型的高流行率，支持该类型与HTLV-I发病的重要关系。

项目成果

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Achiron, A. 等人：“通过对 HAM/TSP 患者和 HTLV I 型携带者的漱口水单核细胞进行原位聚合酶链反应检测 HTLV I 型原病毒” AIDS Res.Hum.Retro。