Pathology and Molecular Evolution of HTLV in Asia

本研究旨在弄清亚洲地区HTLV/STLV感染情况，并对HLAV/STLV的致病关系进行了分子流行病学研究。1)在印度尼西亚的3只猩猩和1只长臂猿身上新发现了STLV-I阳性病例。遗传分析表明，猩猩STLV-I基因变异较大，在系统发育树上形成两个聚类群。HTLV-I的美拉尼西亚群不太可能直接来自猩猩，尽管该群属于亚洲STLV-I大群。2)在印度(凯拉拉400名献血者，卡纳塔克邦20名HAM/TSP患者)、马来西亚(50名献血者在马来西亚北部的吉兰丹)、泰国(泰国南部宋库拉的20名T淋巴瘤患者)和印度尼西亚(220名爱尔兰人、英吉安查亚人)进行了HTLV-I血清筛查。HTLV在接受测试的所有民族中都不是地方病。3)为了研究HTLV分离株之间的分子进化关系，从印度Andra Pradesh地区血清阳性者的淋巴细胞中提取基因组DNA，并以此为模板进行聚合酶链式反应。在安德拉邦的两个独立家系中检测到前病毒基因组。淋巴细胞在IL-2存在下培养。培养6个月后，表达HTLV特异性抗原的细胞出现增殖。4)来自巴基斯坦和阿富汗(含山区少数民族)、阿塞拜疆和阿拉泰的样本各约200份，均为HTLV-I阴性。5)Mashhadi犹太人HAM/TSP患者的HLA型均为B52型，支持了TUPE与HTLV-I致病的重要关系。

The purpose of this resesrch is to clarify the HTLV/STLV infection in Asia and we conducted the molecular epidemiological search and analyzed pathogenic relations of HLA.1) STLV-I positive cases were newly detected on 3 orangutans and 1 gibbon in Indonesia. Genetic analysis showed STLV-I of orangutan was quite variable and made two clusters in phylogenetictree. It is not likely that Melanesian group of HTLV-I directly came from orangutan although the group belongs to big Asian STLV-I group. 2) Seroscreening for HTLV-I was carried out in India (400 blood donors in Kelara, 20 HAM/TSP patients in karnataka), Malaysia (50 donors in Kelantan, Northern Malaysia) , Thailand (20 T-lymphoma patients in Songkhula, Southern Thailand) and Indonesia (220 Iryanese, Inryan Jaya). HTLV was not endemic among none of the ethnic groups tested. 3) In order to study molecular phylogenetic relationships among the HTLV isolates, genomic DNAs were extracted from lymphocytes of seropositive subjects in India, Andra Pradesh, and used as templates in PCR studies. provirus genome was detected in two independent families in Andra Pradesh. The lymphocytes were cultured in the presence of IL-2. After six months in culture, cells expressing HTLV specific antigens showed proliferation. 4) About 200 samples from Pakistan and Afghanistan including minority of mountain area, about 900 samples from Azerbaijan, and about 900 samples from Altai were screened and resulted in all negative for HTLV-I.5) HLA typing of HAM/TSP patients from Mashhadi Jews showed high prevalence of B52 type, supporting the important relations of the tupe to HTLV-I pathogenesis.