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Cino-Japanese cooperative research on surveying genetic variants in Chinese wild mice and developing their inbred strains

中日合作研究中国野生小鼠遗传变异并培育近交系

基本信息

批准号：
07044207
负责人：
MORIWAKI Kazuo
金额：
$ 11.52万
依托单位：
The Graduate University for Advanced Studies
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

In east Asia including mainland China north of Yangtze River, Korea and Japan, a house mouse subspecies, Mus musculis musculus, and some other genetically close ones inhabit. We call them musculus subspecies group. In those regions, p-haplotyoe beta-hemoglobin (Hbb) is frequently observed. In China south of Yangtze River and southeast Asia such as the Philippines, Taiwan and Indonesia, castaneus subspecies group of mice which includes as least two types of Mus musculus castaneus inhabit. In these regions, d-haplotype Hbb is predominant. Genetic divergence between those two subspecies groups has been estimated roughly one million yeare based on molecular genetic analyzes (Yonekawa et al, 1981). Recently we have found a novel Hbb haplotype w1 which is distributed in northwestern region of China with the higher frequency (kawashima et al, 1991). In this study nucleotide siquences of cDNAs in the two hamoglobin genes, b1-w1 and b2-w1, of w1 haplotype were analyzed and compared with those o … More f d haplotype, b1-d and b2-d, and those of p-haplotype, b1-p and b2-p. The sequences of b1-d and b1-p were quite similar, and those of b2-w1 and b2-p as well. On the other hand, several nucleotide substitutions were detected between b1-d and b1-w1 and also between b2-d and b2-w1. These findings suggest a possibility that after differentiation of Mus musculus subspecies, a recombination occurred between d-haplotype and w1-haplotype Hbb chromosomes, which produced p-haplotype with b1-d and b2-w1 genes. Thus, p-haplotype coule be an inter-subspecies recombinant.Further geographical survey of house mouse subspecies carried in the oresent project demonstarated the oresence of d-haplotype of Hbb in east Russia, that is, north of northern China, which suggests possible distribution of castaneus subspecies group in that region in addition to southern China. Twopossibilities could be assumed : (1) In ancient time, castaneus subspecies group of wild mice had once spread their distribution from southeast Asia up to east Russia. Later, musculus subspecies group invaded into the central region of castaneus distribution from west Asia. (2) To east Russia, castaneus subspecies group of mice were recently transported by cargo boats from southeast Asia. Precise analysis of in Asian mouse DNAs is necessary to obtain the more plausible conclusion. Less

在东亚包括中国大陆长江以北、韩国和日本，有家鼠亚种小家鼠Mus musculis musculus和其它遗传关系密切的家鼠。我们称它们为小家鼠亚种群。在这些区域中，经常观察到β-血红蛋白（Hbb）。在我国长江以南及菲律宾、台湾、印度尼西亚等东南亚地区，分布着至少包括两种以上的栗小家鼠（Mus musculus castaneus）亚种群。在这些区域中，d-单倍型Hbb占主导地位。根据分子遗传学分析，这两个亚种群之间的遗传分化估计约为100万年（Yonekawa et al，1981）。最近我们发现了一种新的Hbb单倍型w1，它在中国西北地区分布频率较高（kawashima et al，1991）。本研究分析了w1单倍型血红蛋白基因b1-w1和b2-w1的cDNA序列，并与w1单倍型血红蛋白基因b1-w1和b2-w1的cDNA序列进行了比较。 ...更多信息 f d单倍型b1-d和b2-d，p单倍型b1-p和b2-p，b1-d和b1-p，b2-w1和b2-p的序列相似。另一方面，在b1-d和b1-w1之间以及b2-d和b2-w1之间检测到几个核苷酸替换。这些结果表明，小家鼠亚种分化后，d-单倍型和w1-单倍型Hbb染色体之间发生了重组，产生了含有b1-d和b2-w1基因的p-单倍型。进一步的地理调查表明，Hbb的d-单倍型在俄罗斯东部，即中国北方以北地区存在，这表明栗属亚种群除了在中国南方分布外，还可能在该地区分布。推测有两种可能：（1）在古代，栗属野鼠亚种群的分布曾从东南亚一直扩展到俄罗斯东部。后来，小家鼠亚种群从西亚侵入栗树分布区中部。(2)俄罗斯东部，栗树亚种组的小鼠最近由货船从东南亚运送。对亚洲小鼠DNA的精确分析是获得更合理结论的必要条件。少