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Establishment of laboratory strains originated from Asian wild mice and molecular genetic analysis of the polymorphism

亚洲野生小鼠实验室品系的建立及多态性分子遗传学分析

基本信息

批准号：
07458259
负责人：
MORIWAKI Kazuo
金额：
$ 1.92万
依托单位：
GRADUATE UNIVERSITUY FOR ADVANCED STUDIES
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

From east Asia including mainland China, north of Yangtze River, Korea and Japan, to eastern Europe, a house mouse subspecies, MUS musculus musculus, and some other genetically close ones inhabit. We designated them musculus subspecies group. In southern China, south of Yangtze River, and southeast Asia such as the Philippines, Taiwan and Indonesia, castaneus subspecies group of mice inhabit. In western Europe, domesticus subspecies group is predominant. Genetic divergence among those three subspecies groups has been estimated roughly one million years based on genetic variations in mitochondrial DNAs, ribosomal DNAs, biochemical genes, chromosome C-bands, immunological genes etc. The number of genetic loci so faranalyzed, however, was not enough to obtain highly plausible estimation of genetic divergence among those subspecies groups. The present study attempted to get the more reliable estimation of it by using the larger number of loci. We employed 60 kinds of MIT PCR primers to det … More ect the length polymorphism of microsatellite DNAs. Eight kinds of inbred strains established in National Institute of Genetics in Mishima from various wild mice covering the three major subspecies groups were used in the present analyzes. MAL strain originated from Malysia, CAST from Thailand and HMI from Taiwan in castaneus subspcies group, MSM from Japan, SWN and KJR from Korea, BLG2 form Bulgaria and NJL from Denmark in musculus group, and BFM from France in domesticus group were analyzed. Dendrogram constructed from the length polymorphism of microsatellite DNAs by NJ method well agreed with the previous one mentioned above. Only discrepancy in this analysis with the previous one was BLG2 which was estimated as domesticus group, not musculus one, Since the number of lici compared in the present assay were considerably large, BLG2 should be included in domesticus subspecies group, though the genetic introgression of musculus group to some extent could not be excluded. Estimation of genetic distance between two populations based on the relatively larger number of polymorphic microsatellite DNAs seems to be considerably reliable. Less

从东亚包括中国大陆、长江以北、朝鲜和日本，到东欧，有家鼠亚种MUS musculus musculus和其他一些遗传上接近的家鼠。我们把它们命名为小家鼠亚种群。在中国南方、长江以南以及菲律宾、台湾、印度尼西亚等东南亚地区，均有栗鼠亚种群的分布。在西欧，南极亚种群占主导地位。根据线粒体DNA、核糖体DNA、生化基因、染色体C-带、免疫基因等的遗传变异，估计这三个亚种群之间的遗传分化大约有100万年。然而，迄今为止分析的遗传位点的数量还不足以获得这些亚种群之间高度可信的遗传分化估计。本研究试图通过使用更多的位点来获得更可靠的估计。我们采用60种MIT PCR引物， ...更多信息检测微卫星DNA的长度多态性。本分析使用了三岛国立遗传学研究所从涵盖三个主要亚种群的各种野生小鼠中建立的8种近交系。对板栗亚种群的MAL株、泰国CAST株和台湾HMI株、小家鼠群的日本MSM株、韩国SWN株和KJR株、保加利亚BLG 2株和丹麦NJL株以及法国BFM株进行了分析。用NJ法构建的微卫星DNA长度多态性树状图与上述结果吻合较好。与前一次分析结果的差异仅在于BLG 2，它被估计为小家鼠组，而不是小家鼠组。由于本次分析中比较的地衣数量相当大，BLG 2应被包括在小家鼠亚种组中，但不能排除小家鼠组在一定程度上的遗传渐渗。利用相对较多的多态性微卫星DNA估计两个居群间的遗传距离是相当可靠的。少