Nutritional studies related to the development of mass production techniques of quality larvae

优质幼虫批量生产技术开发相关营养研究

• 批准号: 07306009
• 负责人: WATANABE Takeshi
• 金额: $ 18.18万
• 依托单位: Tokyo University of Fisheries
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07306009/
• 关键词: Yellowtail; Japanese flounder; Red sea bream; Striped Jack; Vitamin C; Docosahexaenoic acid; Dry pellet; Micro feed for fish larva; 健苗育成; クルマエビ; マダラ; ハタ

One of the most important and fundamental approaches to the successful mass propagation of quality larvae is to cultivate quality broodstock which can produce fertile eggs and to culture hatched larvae with quality formulated microdiets or live foods. From this viewpoint several nutritional studies have been carried out in this two-year project. The results obtained are summarized as follows.With regard to broodstock diet, a newly-developed soft-dry pellet (SDP) was found to be suitable for yellowtail and supplementation of 30ppm astaxanthin to SDP enhanced spawning and quality of eggs. As for the data on nutritional requirements essential for development of microdiets, experiments were conducted on vitamin C (VC) and thiamine. In larval flounder VC requirement was affected by dietary n-3 HUFA levels, and was 10mg/100g diet when n-3HUFA was 2% in diet. In red seabream larvae TBA value of muscle suggested that VC can reduce lipid peroxidation in muscle. The thiamine requirement of larval Kuruma shrimp was found to be around 14mg/100g diet based on the maximum growth and the hepatopancreatic thiamine pyrophosphate content. On the other hand, in experiments with live foods. DHA requirement was determined for some marine larval fish at the stage subsisting on Artemia. On a dry matter basis of Artemia, the DHA requirement was 0.95-1.62% for red seabream, 1.39-2.63% for yellowtail and 1.6-2.2% for striped jack. These results are valuable information for establishment of techniques related to mass seed production of various species.

成功质量幼虫的成功大规模繁殖的最重要和基本的方法之一是培养可以产生肥沃卵的质量生育，并用质量配方的微载体或活食物孵化幼虫。从这个角度来看，这项为期两年的项目已经进行了一些营养研究。所获得的结果总结如下。关于育雏饮食，发现新开发的软干燥颗粒（SDP）适用于黄尾巴，并补充30ppm astaxanthin to SDP增强了卵子和鸡蛋的质量。至于关于营养需求的数据，对微产物的发展至关重要，对维生素C（VC）和硫胺素进行了实验。在幼虫中，比目鱼的需求受膳食N-3 HUFA水平的影响，当N-3HUFA的饮食饮食为2％时，饮食中的饮食水平为10mg/100g。在红色Seabream幼虫中，肌肉的TBA值表明VC可以减少肌肉中的脂质过氧化。根据最大生长和肝癌硫胺素焦磷酸含量，发现幼虫kuruma虾的硫胺素需求约为14mg/100g饮食。另一方面，在实验室实验中。确定了一些阶段的海洋幼虫鱼的需求。在干物质的基础上，红色Seabream的DHA需求为0.95-1.62％，黄尾尾的1.39-2.63％，条纹千斤顶为1.6-2.2％。这些结果是建立与各种物种大量种子生产有关的技术的宝贵信息。

项目成果

T.Watanabe, V.Verakunpiriya, K.Mushiake, K.Kawano, and I.Hasegawa: "The first spawn-taking from broodstock yellowtail cultured with extruded pellets" Fisheries Science. 62(3). 388-393 (1996)

T.Watanabe、V.Verakunpiriya、K.Mushiake、K.Kawano 和 I.Hasekawa：“首次从用挤压颗粒养殖的亲鱼黄尾鱼中产卵”渔业科学。

T.Takeuchi, R.Masuda, Y.Ishizaki, T.Watanabe, M.Kanematsu, K.Imaizumi, and K.Tsukamoto: "Determination of the requirement of larval striped jack for eicosapentaenoic acid and docosahexaenoic acid using enriched Artemia nauplii" Fisheries Science. 62(5). 7

T.Takeuchi、R​​.Masuda、Y.Ishizaki、T.Watanabe、M.Kanematsu、K.Imaizumi 和 K.Tsukamoto：“使用富集卤虫无节幼体测定条纹杰克幼虫对二十碳五烯酸和二十二碳六烯酸的需求”渔业

H.Furuita, T.Takeuchi, M.Toyota, and T.Watanabe: "EPA and DHA requirements in early juvenile red seabream using HUFA enriched Artemia nauplii" Fisheries Science. 62(2). 246-251 (1996)

H.Furuita、T.Takeuchi、M.Toyota 和 T.Watanabe：“使用富含 HUFA 的丰年虫无节幼体对早期幼红鲷的 EPA 和 DHA 要求”渔业科学。

T.Watanabe et al.: "The first spawn-taking from broodstock yellowtail cultured with extruded pellets" Fisherics Science. 62. 388-393 (1996)

T.Watanabe 等人：“首次从用挤压颗粒养殖的亲鱼黄尾鱼中产卵”渔业科学。

V.Verakunpiriya, T.Watanabe, K.Mushiake, V.Kiron, S.Satoh, and T.Takeuchi: "Effect of broodstock diets on the chemical components of milt and eggs produced by yellowtail" Fisheries Science. 62(4). 610-619 (1996)

V.Verakunpiriya、T.Watanabe、K.Mushiake、V.Kiron、S.Satoh 和 T.Takeuchi：“亲鱼饲料对黄尾鱼产生的鱼白和鱼卵化学成分的影响”渔业科学。