Studies on the origin of the monogenean Neoheterobothrium hirame, the causative agent of parasitic anemia of Japanese flounder

日本牙鲆寄生性贫血病原单殖期新异博氏菌的起源研究

基本信息

批准号：
15405027
负责人：
OGAWA Kazuo
金额：
$ 3.58万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

The monogenean Neoheterobothrium hirame suddenly appeared in Japanese flounder in the waters adjacent to Japan in mid-1990s, causing parasitic anemia in wild flounder. The impact of the parasite on the wild stocks of Japanese flounder is much apprehended. In this study, in order to clarify the origin of the parasite, several species of genus Neoheterobothrium were collected from southern flounder, Paralichthys lethostigma, and summer flounder, P.dentatus, distributed in the Atlantic waters of USA and bigeye flounder, Hippoglossina macrops distributed in Chile and compared with N.hirame.Worms collected from southern flounder were virtually identical to N.hirame in the morphology and sequences of 18S rRNA region, ITS1-5.8S rRNA- ITS2 region, and mitcondrial COI region. From the results, it is concluded that N.hirame recently invaded Japanese waters and settled there using Japanese founder as its host.According to a previous report that southern flounder was infected with N.affine originally reported from summer flounder, there was a possibility that N. hirame was synonymous to N.affine. In this study, two specimens of N.affine were obtained from summer flounder and compared with N.himame morphologically and molecular biologically. The present results indicate that the two species belong to different species, although further studies are needed for reaching a conclusion because of the small number of the specimens used in this study.Neoheterobothrium chilensis collected fom H.macrops was greatly different from N.hirame and N.affine in the morphology and the sequences of ITS1 and 28S rRNA resions. The results suggest that N.chilensis is not congeneric to the two species.

一元新的丘脑hirame突然出现在1990年代中期与日本附近的日本比目鱼中，引起了野生比目鱼的寄生贫血。寄生虫对日本比目鱼的野生股票的影响令人震惊。在这项研究中，为了澄清寄生虫的起源，从南部的fllounder，paralichthys lethostigma和萨默·弗洛德（P.Dentatus）（dentatus）中收集了几种种类的新植物，并从美国的大西洋水域中分布在美国的大西洋水域，与Bigeye fllounder，hippoglossina macrops collediate n.n. collound frol n。 N. hirame在18S rRNA区域，ITS1-5.8S rRNA-ITS2区域和Mitcondrial COI区域的形态和序列中。从结果得出的结论是，N.Hirame最近入侵了日本水域，并以日本创始人为主机定居在那里。根据先前的报告，即南方Flounder被N.Affine感染了N.Affine最初是从Summer Flounder那里报道的，有可能是N. Hirame是N. Affine的代名词。在这项研究中，从夏季比目鱼中获得了两个n. affine标本，并与形态和分子生物学上的n.himame进行了比较。目前的结果表明，这两个物种属于不同的物种，尽管由于本研究中使用的标本数量很少，因此需要进一步的研究来得出结论。奇氏奇质收集的fom h. -Macrops与n.hirame和N. affine的形态和ITS1和28S RRNA的序列中的N.Hirame和N.Affine大不相同。结果表明，n. chilensis对这两个物种不是同类。