MOLECULAR ANATOMY OF TRANSCRIPTION APPARATUS

转录装置的分子解剖学

• 批准号: 08044107
• 负责人: ISHIHAMA Akira
• 金额: $ 1.34万
• 依托单位: NATIONAL INSTITUTE OF GENETICS
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044107/
• 关键词: RNA polymerase ;; transcription factor :; DNA-protein interaction; protein-protein interaction ;; molecular assembly ;; structure-function relation ;; Escherichia coli ;; transcription regulation; 蛋白質-蛋白質相互作用

RNA polymerase plays a key role in gene transcription. Transcription apparatus in both prokaryotes and eukaryotes are composed of RNA polymerase and one or more of transcription factors. The promoter selectivity of prokaryotic RNA polymerases is modulated in two steps of molecular communications, i.e., the first step with one of sigma factors and the second step with one or more of the transcription factors. In order to understand the regulatory mechanisms of promoter selectivity of the RNA polymerase, protein-protein contact sites between subunits and between subunits and tranascription factors have been mapped by both genetic and biochemical methods. Results indicated that : the concentration and activity of seven different sigma factrs involved in the first-step differentiation in Escherichia coli vary depending on cell growth conditions ; there are about 100 different species of the second-step factors in E.coli, which can be classified into four groups depending on the contact sites on RNA polymerase subunits ; more than two factors are able to interact with the RNA polymerase independently.

RNA聚合酶在基因转录中起着关键作用。原核生物和真核生物的转录器都是由RNA聚合酶和一种或多种转录因子组成。原核RNA聚合酶的启动子选择性在分子通讯的两个步骤中被调节，即，第一步用一种σ因子，第二步用一种或多种转录因子。为了了解RNA聚合酶启动子选择性的调控机制，蛋白质-蛋白质接触位点之间的亚基和亚基和转录因子已被定位的遗传和生物化学方法。结果表明：大肠杆菌中参与第一步分化的七种不同σ因子的浓度和活性根据细胞生长条件而变化;大肠杆菌中有大约100种不同种类的第二步因子，根据RNA聚合酶亚基上的接触位点可将其分为四组;两种以上的因子能够独立地与RNA聚合酶相互作用。

项目成果

Jishage,M.: "Variation in RNA polymerase sigma subunit composition within different stocks Escherichiacoli strain W3110." J.Bacteriol.179・3. 959-963 (1997)

Jishage, M.：“不同种大肠杆菌菌株 W3110 中 RNA 聚合酶 sigma 亚基组成的变化。J.Bacteriol.179·3 (1997)”

Kusano, S.: "Promoter selectivity of Escherichia coli RNA Polymerase Eσ^<70> and Eσ^<38> holoenzymes: Effect of DNA supercoiling." J. Biol. Chem.271(4). 1998-2004 (1996)

Kusano, S.：“大肠杆菌 RNA 聚合酶 Eσ^<70> 和 Eσ^<38> 全酶的启动子选择性：DNA 超螺旋的影响。J. Biol。271(4) (1996)。

Arisimovitch, I.: "Transcription activation by the bacteriophage Mu Mor protein requires the C-terminal regions of both alpha and sigma^<70> subunits of Escherichiacoli RNA polymerase." J.Biol.Chem.271. 32343-32348 (1996)

Arisimovitch, I.：“噬菌体 Mu Mor 蛋白的转录激活需要大肠杆菌 RNA 聚合酶的 α 和 sigma^<70> 亚基的 C 末端区域。”

Jair, K.-W.: "Ambidextrous transcription activation by SosS : Requirement for the C-terminal domain of the RNA polymerase alpha subunit at a subset of superoxide inducible genes." Mol.Microbiol.19(2). 307-314 (1996)

Jair, K.-W.：“SosS 的双手灵巧的转录激活：对超氧化物诱导基因子集的 RNA 聚合酶 α 亚基的 C 端结构域的要求。”

Artsimovitch,I.: "Transcription activation by the bacteriophage Mu Mor protein requires the C-terminal regions of both α and σ^<70> subunits of Escherichiacoli RNA polymerase." J.Biol.Chem.271. 32343-32348 (1996)

Artsimovitch, I.：“噬菌体 Mu Mor 蛋白的转录激活需要大肠杆菌 RNA 聚合酶的 α 和 σ^<70> 亚基的 C 端区域。J.Biol.Chem.271 (1996)。