Functional Genomics

功能基因组学

• 批准号: 08283105
• 负责人: KOHARA Yuji
• 金额: $ 344.26万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08283105/
• 关键词: gene expression; FST; RNAi; full-length cDNA; expression pattern; phenotype; gene disruption; protein-protein interaction; 遺伝子発現プロファイル; ボディマップ; ATAC-PCR; 遺伝子発現パターン; 酵母two-hybrid法; cDNA; 線虫; 出芽酵母; 枯草菌; ディファレンシャルディスプレイ

Y. Kohara carried out the systematic analysis of expression patterns of 7000 genes along the time course of development of the nematode C. elegans, and performed the clustering analysis to identify the cis-elements of various regulatory regions. K. Okubo revealed the structure of human transcriptome through the construction of the database named BodyMap which consisted of the expression patterns with respect to anatomy of some 20,000 human genes. S. Sugano developed the Oligo-capping method aiming at constructing full-length enriched cDNA libraries and applied it to the systematic analysis of the transcription start sites in the human genome. T. Itoh established a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast S, cerevisiae proteins, and predicted several gene cascades base on the identified interactions. N. Ogasawara and collaborators carried out functional analysis of B. subtilis genome through the disruption and phenotype analysis on 2674 genes as a Japan-Europe joint project. K. Kato developed a highly sensitive method named Adapter-tagged Competitive PCR and applied it to gene expression profiling of mouse postnatal cerebellar development. Other projects were also performed as follows. E. Takahashi performed functional analysis of 162 human genes. T. Aigaki generated 2000 GS (gene-search) strains of D. melanogaster to screen various genes. S. Minani developed a highly effective method to generate deletion mutants in C. elegans. A. Sugimoto improved the soaking RNAi method and performed the phenotype analysis of 2500 C. elegans genes. M. Maeda and her collaborators carried out cDNA project of the slime mold D. discoideum. Y. Murakami carried out expression profiling of the all genes of the chromosome VI of S. cerevisiae.

Y. Kohara对线虫C.的发育过程中沿着的7000个基因的表达模式进行了系统分析。elegans的基因组序列，并进行聚类分析，以确定各种调控区的顺式元件。K.大久保通过构建名为BodyMap的数据库揭示了人类转录组的结构，该数据库由大约20，000个人类基因的解剖学表达模式组成。S. Sugano等人发展了Oligo-capping方法，旨在构建全长富集的cDNA文库，并将其应用于人类基因组转录起始位点的系统分析。T. Itoh建立了一个全面的系统来检测酵母酿酒酵母蛋白之间所有可能的组合中的双杂交相互作用，并根据所鉴定的相互作用预测了几个基因级联。N.小笠原和合作者对B进行了功能分析。作为日本-欧洲联合项目，通过对2674个基因的破坏和表型分析，对枯草杆菌基因组进行了研究。K. Kato发展了一种高灵敏度的衔接子标记竞争性PCR方法，并将其应用于小鼠出生后小脑发育的基因表达谱分析。还执行了以下其他项目。E. Takahashi对162个人类基因进行了功能分析。T. Aigaki获得了2000株D.筛选各种基因。S. Minani开发了一种高效的方法来产生C.优美的A. Sugimoto等人改进了浸泡RNAi方法，并对2500株C. elegans基因M. Maeda和她的合作者进行了黏菌D.盘状突Y. Murakami对S.啤酒。

项目成果

Masuda N: "Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples"Nucleic Acids Res.. 27(22). 4436-43 (1999)

Masuda N：“福尔马林固定样品中 RNA 的化学修饰分析以及此类样品的分子生物学应用的优化”《核酸研究》27(22)。

Harada Y.: "Complete cDNA sequence and genomic organization of a human pancreas-specific gene homologous to Caenorhabiditis elegans sel-1"J. Hum Genet. 44. 330-336 (1999)

Harada Y.：“与秀丽隐杆线虫 sel-1 同源的人类胰腺特异性基因的完整 cDNA 序列和基因组结构”J.

Yamamoto H.: "Determination of a 12kb nucleotide sequence around the 76 degree region of the Bacillus subtilis chromosome" Microbiol.142. 1417-1421 (1996)

Yamamoto H.：“枯草芽孢杆菌染色体 76 度区域周围 12kb 核苷酸序列的测定”Microbiol.142。

Yu Y: "Human papillomavirus type 33 DNA in breast cancer in chinase"Breast Cancer. 7(1). 33-36 (2000)

于Y：“中国乳腺癌中的人乳头瘤病毒33型DNA”乳腺癌。

Toba, G.: "Disruption of the Microsomal glutathione S-transferase-like gene reduces life span of Drosophila melanogaster"Gene. 253. 179-187 (2000)

Toba, G.：“微粒体谷胱甘肽 S-转移酶样基因的破坏会缩短果蝇的寿命”基因。