Functional Genomics

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Y. Kohara carried out the systematic analysis of expression patterns of 7000 genes along the time course of development of the nematode C. elegans, and performed the clustering analysis to identify the cis-elements of various regulatory regions. K. Okubo revealed the structure of human transcriptome through the construction of the database named BodyMap which consisted of the expression patterns with respect to anatomy of some 20,000 human genes. S. Sugano developed the Oligo-capping method aiming at constructing full-length enriched cDNA libraries and applied it to the systematic analysis of the transcription start sites in the human genome. T. Itoh established a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast S, cerevisiae proteins, and predicted several gene cascades base on the identified interactions. N. Ogasawara and collaborators carried out functional analysis of B. subtilis genome through the disruption and phenotype analysis on 2674 genes as a Japan-Europe joint project. K. Kato developed a highly sensitive method named Adapter-tagged Competitive PCR and applied it to gene expression profiling of mouse postnatal cerebellar development. Other projects were also performed as follows. E. Takahashi performed functional analysis of 162 human genes. T. Aigaki generated 2000 GS (gene-search) strains of D. melanogaster to screen various genes. S. Minani developed a highly effective method to generate deletion mutants in C. elegans. A. Sugimoto improved the soaking RNAi method and performed the phenotype analysis of 2500 C. elegans genes. M. Maeda and her collaborators carried out cDNA project of the slime mold D. discoideum. Y. Murakami carried out expression profiling of the all genes of the chromosome VI of S. cerevisiae.