Expression-pattern based studies on gene regulation network in the nemetode C.elegans

基于表达模式的线虫基因调控网络研究

• 批准号: 22241047
• 负责人: KOHARA Yuji
• 金额: $ 24.71万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22241047/
• 关键词: 遺伝子; 発現調節; ゲノム; 発生分化; mRNA; 3'-UTR; 局在化; シグナル伝達

We have found the cis- and trans-elements for the localization of pos-1 mRNA to the posterior side and mex-3 mRNA to the anterior side in the 2 cell stage embryo of C.elegans, and have obtained the clue to understand the mechanisms of mRNA localization. 2) Based on the analysis of β-catenin HMP-2 in C.elegans early embryos, we have proposed that HMP-2 functioned downstream of the Src signaling pathway and contributed to endoderm induction and ABar spindle orientation, in parallel with the Wnt signaling pathway. 3) We identified the regions of promoter activity for gcy-8 and gcy-18 , essential genes for the thermosensory neuron AFD, and found that the transcription factors CEH-11 and TTX-1 functioned in the regulation of the gene expression coordinately

我们发现了在秀丽隐杆线虫2细胞期胚胎中pos-1 mRNA定位于后侧和mex-3 mRNA定位于前侧的顺式和反式元件，为理解mRNA定位机制提供了线索。2)基于对秀丽隐杆线虫早期胚胎中β-catenin HMP-2的分析，我们提出HMP-2在Src信号通路下游发挥作用，与Wnt信号通路平行，参与内胚层诱导和ABar纺锤体定向。3)我们鉴定了热感觉神经元AFD的必需基因gcy-8和gcy-18的启动子活性区域，发现转录因子CEH-11和TTX-1协同调控该基因的表达

项目成果

線虫C.elegansの温度感受神経AFDでの発現を制御する調節配列の解析

线虫温度敏感神经 AFD 表达调控序列分析

• 发表时间: 2010
• 作者: Goto;K.;Yuichi Hongoh;鹿児島浩
• 通讯作者: 鹿児島浩

The landscape of C. elegans 3'UTRs.

• DOI: 10.1126/science.1191244
• 发表时间: 2010-07-23
• 期刊: Science (New York, N.Y.)
• 作者: Mangone M;Manoharan AP;Thierry-Mieg D;Thierry-Mieg J;Han T;Mackowiak SD;Mis E;Zegar C;Gutwein MR;Khivansara V;Attie O;Chen K;Salehi-Ashtiani K;Vidal M;Harkins TT;Bouffard P;Suzuki Y;Sugano S;Kohara Y;Rajewsky N;Piano F;Gunsalus KC;Kim JK
• 通讯作者: Kim JK

The homeobox transcription factors, CEH-14 and TTX-1, regulate AFD thermosensory neuron-specific expression of gcy-8 and gcy-18 in C.elegans

同源盒转录因子 CEH-14 和 TTX-1 调节线虫中 gcy-8 和 gcy-18 的 AFD 热感觉神经元特异性表达

• 发表时间: 2012
• 作者: 鹿児島浩;小原雄治
• 通讯作者: 小原雄治

Kampkotter A, Bossinger O.: C. elegans VANG-1 Modulates Life Span via Insulin/IGF-1-Like Signaling

Kampkotter A、Bossinger O.：线虫 VANG-1 通过胰岛素/IGF-1 样信号调节寿命

• 发表时间: 2012
• 期刊: PLoS One
• 影响因子: 3.7
• 作者: Honnen SJ;Buchter C;Schroder V;Hoffmann M;Kohara Y
• 通讯作者: Kohara Y

In situ hybridization analysis of let-7 microRNA. 18th International C.elegans Meeting

let-7 microRNA 的原位杂交分析。

• 发表时间: 2011
• 作者: Y. Andachi;Y. Kohara
• 通讯作者: Y. Kohara