Large-scale DNA sequencing and its application

我们通过对测序过程的审查、改进和成本的逐步降低，建立了一个大规模的(Sanger)DNA测序系统，使我们每年获得600万次(Sanger)读数。根据资助小组研究人员的要求，我们为16个生物的92个cDNA文库制作了340万个EST阅读器。我们还为7种生物的全基因组鸟枪测序产生了1940万个读数，其中包括野生小鼠、半角鼠、水熊、尾鞭毛虫卵形无尾轮虫。在项目期间，总共制作了2400万份阅读器。为了更仔细地分析这些读数，我们开发了一个基因组完成工作台和一个用于多态鉴定的查看器。对于下一代DNA测序系统，我们建立了一个具有个人基因组学必不可少的安全系统的计算机系统，并进行了各种DNA测序。此外，我们还开发了用于多态鉴定的查看器系统和cDNA全测序系统。对Solexa多路数据分析也进行了检验。

We established a large-scale (Sanger) DNA sequencing system through the review, improvement and cost reduction of the sequencing process step by step, leading to allow us to obtain 6 million (Sanger) reads per year. Based on the requests from the researchers in the grant group, we produced 3.4 million EST reads for 92 cDNA libraries from 16 organisms. We also produced 19.4 million reads for whole genome shotgun sequencing of 7 organisms including wild-derived mouse, a hemichordata, water bears, the choanoflagellate Monosiga ovata. Altogether 24 million reads were produced during the project period. To analyze the reads more closely, we developed a work-bench for genome finishing and a viewer for polymorphism identification. For the next generation DNA sequencing system, we set up a computer system with a security system that is essential for personal genomics and performed various DNA sequencing. Furthermore, we developed a viewer system for polymorphism identification and a system for cDNA full sequencing. Solexa multiplexing data analysis was also tested.