Effects of environmental endocrine disrupters on learning, memory and emotional behavior in the function of central nervous system

环境内分泌干扰物对中枢神经系统功能学习记忆和情绪行为的影响

• 批准号: 12836011
• 负责人: YAMASHITA Kimihiro
• 金额: $ 2.62万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12836011/
• 关键词: environmental endocrine disruptors; TCDD; nonylphenol; i. c. v. injection; locomotor activity; learning and memory; open-field test; central nervous system; ラット脳; 摂食量; 飲水量

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is considered to be one of the most potent environmental endocrine disruptors. TCDD has been demonstrated to be a potent carcinogen and have immunotoxic effects. It is still uncertain, however, whether dioxins have also toxic properties in the central nervous system. Nonylphenol (NP), an alkylphenol polyethoxylates, is widely used as components of detergents, paints and many other synthetic products. This synthetic chemical is also considered to be an environmental endocrine disrupter that acts as an estrogenic substance. The present study was designed to investigate the effects of TCDD and NP on the central nervous system in male SD rats. Rats were received single i. c. v. administration of TCDD (100 ng/ 2μl) or NP (2 μg/ 2μl) at 8 weeks-old, and locomotor activities, food consumption and water intakes were measured. We also investigated effects of TCDD and NP on memory and emotional impairments in rats using 8 arm radial maze and Open-field apparatus, respectively. Under 12 hour-light/dark condition, the TCDD-, but not NP-treated rats exhibited higher locomotor activities without altering circadian rhythm. However, this dose of these endocrine disruptors did not affect the maze performance. These results suggest that TCDD may affect in a central nervous system to induce hyperactivity in rats.

2，3，7，8-四氯二苯并对二恶英（TCDD）被认为是最强的环境内分泌干扰物之一。TCDD已被证明是一种强致癌物，并具有免疫毒性作用。不过，二恶英是否对中枢神经系统也有毒性，目前仍不确定。壬基酚（NP）是一种烷基酚聚氧乙烯醚，广泛用作洗涤剂、涂料和许多其他合成产品的组分。这种合成化学品也被认为是一种环境内分泌干扰物，作为雌激素物质发挥作用。本研究旨在观察TCDD和NP对雄性SD大鼠中枢神经系统的影响。大鼠接受单次i. C.在8周龄时分别给予TCDD（100 ng/ 2μl）或NP（2 μg/ 2μl），测定小鼠的自主活动、摄食量和饮水量。本研究还分别采用八臂迷宫和旷场实验研究了TCDD和NP对大鼠记忆和情绪障碍的影响。在12小时光照/黑暗条件下，TCDD处理的大鼠，而不是NP处理的大鼠表现出较高的运动活动，而不改变昼夜节律。然而，这些内分泌干扰物的剂量并不影响迷宫的表现。这些结果表明，TCDD可能影响中枢神经系统，导致大鼠多动症。

项目成果

H.Kurita: "Effects of environmental endocrine disruptors on central nervous systems in SD rats"The 7th Joint Symposium of Cheju National University and Nagasaki University on Science and Technology. (in press).

H.Kurita：“环境内分泌干扰物对SD大鼠中枢神经系统的影响”第七届济州大学和长崎大学科学技术联合研讨会。

H. Kurita: "Effects of environmental endocrine disrupters on central nervous systems in SD rats"The 7 th Joint Symposium of Cheju National University and Nagasaki University on Science and Technology. (in press).

H.栗田：“环境内分泌干扰物对SD大鼠中枢神经系统的影响”第七届济州大学和长崎大学科学技术联合研讨会。

K. Yamashita: "Involvement of glial endothelin/nitric oxide in delayed neuronal death of rat hippocampus after transient forebrain ischemia"Cell. Mol. Neurobiol.. 20. 541-551 (2000)

K. Yamashita：“神经胶质内皮素/一氧化氮参与短暂前脑缺血后大鼠海马延迟性神经元死亡”细胞。

K.Yamashita: "Involvement of glial endothelin/nitric oxide in delayed neuronal death of rat hippocampus after transient forebrain ischemia"Cell. Mol. Neurobiol.. 20.5. 541-551 (2000)

K.Yamashita：“神经胶质内皮素/一氧化氮参与短暂前脑缺血后大鼠海马延迟性神经元死亡”细胞。

H.Kurita: "Effects of environmental endocrine disruptors on central nervous systems in SD rats"The 7 th Joint Symposium of Cheju National University and Nagasaki University on Science and Technology. (in press).

H.Kurita：“环境内分泌干扰物对SD大鼠中枢神经系统的影响”第七届济州大学和长崎大学科学技术联合研讨会。