Visualization and quantification of intracellular magnesium ion to determine the influence of magnesium to pragnancy circulation

本项目的目的之一是开发一种可以可视化和量化细胞内镁离子水平的显微测量系统，并将该系统应用于妊娠循环中镁离子动态的研究。为了可视化细胞内镁离子，荧光探针（Mag-fra2）与具有两个激发波长的微型分光光度计一起使用，以获得荧光发射的强度比。由于钙离子水平和镁离子水平在细胞内是竞争的，所以钙离子水平和镁离子水平必须用同一仪器测量。试验装置已经完成。研究了子痫前期孕妇血栓细胞镁离子水平与妊娠的关系。该项目已扩展到量化人体血管中的钙。用原子发射光谱法（AES）对钙进行了定量分析。为此，研制了一种微波诱导耦合AES系统。下肢钙含量高于上肢。提示钙含量与动脉硬化之间可能存在相关性。动脉内静脉、关节部分钙含量高于其他部位。这表明钙含量与运动（生活习惯）、血压、血湍流等机械应力之间可能存在相关性。此外，还开发了一种可视化活样中分子构象变化的新技术。该技术基于显微光学和相干反斯托克斯拉曼光谱（CARS）。为了建立微观CARS方法，研究了CARS系统的光学传递函数。

One aim of this project is to develop a microphotometric measurement system that can visualize and quantify the intracellular magnesium ion level, and to apply this system into the study on the dynamics of magnesium ion during the pregnancy circulation. To visualize the intracellular magnesium ion, a fluorescent probe (Mag-fra2) is used in conjunction with a micro spectrophotometer that has two excitation wavelengths to take an intensity ratio of the fluorescence emission. Because the calcium ion level and the magnesium ion level is competetive in the cell, both calcium level and magnesium level must be measured with the same apparatus. The test apparatus has been completed. Relationship between the magnesium ion level in thrombocyte and the pregnancy has been investigated in preeclamptic pragnant women.The project has been extended to quantify the calcium in human blood vessels. The calcium is quantified by atomic emission spectroscoy (AES). For this, a microwave-induced coupled AES system has been made. The calcium content of lower limbs was higher than that of upper limbs. This result indicates that correlation between Ca content and arteriosclerosis maybe exists. Ca was much contained in parts of veinlet and joint in the artery comparing with other portion. This indicates that correlation between Ca content and mechanical stress such as exercise (living custom), blood pressure, and blood turbulent flow maybe exists.Also a new technique that can visualize the conformation change of molecules in living sample has been developed. This technique is based on the microscopic optics and coherent anti-Stokes Raman spectroscopy (CARS). To establish the microscopic CARS method, optical transfer function of the CARS system has been studied.