Multimodal molecular imaging system and its application for investigation of in vivo cell/tissue

本研究计划的目的是开发和应用新的成像方法，可以告知生物样品的分子形态和功能。 本论文主要研究了二次谐波显微技术和阴极发光显微技术，并研制了一台偏振分辨的高速二次谐波显微镜。 用该显微镜进行活体测量，观察与年龄相关的真皮胶原图像。我们还展示了由兔软骨细胞和I型胶原凝胶组成的组织工程软骨的SHG成像。 由此，我们证实，它是适合于评价组织工程软骨的质量。在化学发光显微镜中，合成了三种稀土掺杂的纳米荧光体，并将其注射到巨噬细胞中。纳米荧光粉的空间分布是可视化的，通过使用扫描电子显微镜CL系统。

Aims of this research project are development and application of novel imaging method that can informs molecular morphology and function of biological specimens. In the present research, we have focused on the second harmonic generation (SHG) microscopy and cathode luminescence(CL) microscopy.A polarization resolved, high-speed SHG microscope was developed. In in vivo measurement with this microscope, age related dermis collagen image were observed. Also we demonstrated SHG imaging of tissue-engineered cartilage composed of rabbit chondrocytes and type I collagen gel. From this, we confirmed that it is suitable for evaluating the quality of tissue-engineered cartilage. In the CL microscopy, three kinds of rare-earth-doped nanophosphors were synthesized and injected into macrophages. The spatial distribution of nanophosphors was visualized by using a scanning electron microscope CL system.