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Heterogeneity of auto-fluorescence of human tooth tissue progressed by aging

衰老过程中人类牙齿组织自发荧光的异质性

基本信息

批准号：
05671714
负责人：
ARAKI Tsutomu
金额：
$ 1.34万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671714/
关键词：
HUMAN TOOTH FLUORESCENCE TIME-RESOLVED FLUOROMETER AGING 老化歯牙

项目摘要

Age changes of auto-fluorescence intensities of six regions of buccolingual and longitudinal directions in human dentin were studied using a time-resolved fluorescence microphotometer. Static measurement mode showed that fluorescence intensity increased with aging in the all regions. Wavelength of the maximum fluorescence and full width of half maximum of fluorescence spectrum, however, did not change ; the values of the former and the latter were around 440nm and about 100nm, respectively, in the all regions of all teeth studied. The dynamic mode suggested that the auto-fluorescence of 440nm wavelength had at least three fluorescent components with different fluorescence decay times, fast, intermediate and slow. Fluorescence intensities of these components increased with aging in the six regions ; the intensity of the fast component showed an increase in proportion to aging after 18 years old, while in the other two components, the increasing rate of fluorescence intensities decreased at old aged teeth. Age changes of ratios of intensities of the three components to the static fluorescent intensities were distinguishable among the regions of longitudinal direction ; the ratio of the fast component increased with aging in the coronal dentin, while in the root apex dentin, the ratio of the slow one decreased. A tendency intermediate between the former two regions was seen in the cervical dentin. The above results showed that patterns of age changes were different in the longitudinal direction, from which it was inferred that factors affecting age changes were different between the coronal and root apex dentins.

采用时间分辨荧光显微光度计研究了人牙本质颊舌向和纵向六个区域自发荧光强度的年龄变化。静态测量模式显示，荧光强度在所有区域随着老化而增加。而荧光光谱的最大荧光波长和半峰全宽没有变化，在所研究的所有牙齿的所有区域中，前者和后者的值分别在440 nm和100 nm左右。动力学模式表明，440 nm波长的自发荧光至少有三种荧光成分，它们具有不同的荧光衰减时间，即快、中、慢。这6个区域的荧光强度均随年龄增长而增加，其中快成分的荧光强度在18岁以后随年龄增长而增加，而另外两个成分的荧光强度在老年牙区的增加率则有所下降。3种成分强度与静态荧光强度比值的年龄变化在牙本质纵向各区域间有明显差异，在冠状面牙本质中，快成分强度与静态荧光强度比值随年龄的增加而增加，而在根尖牙本质中，慢成分强度与静态荧光强度比值随年龄的增加而降低。牙颈部的牙本质有一种介于前两个区域之间的趋势。以上结果表明，牙本质在纵向上的年龄变化规律不同，由此推断，影响牙本质年龄变化的因素在冠、根尖牙本质中存在差异。