Role of Nitric Oxide on Minimum Alveolar Anesthetic Concentration

一氧化氮对最低肺泡麻醉浓度的作用

• 批准号: 10671398
• 负责人: FUKUDA Taeko
• 金额: $ 1.15万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671398/
• 关键词: Nitric Oxide (NO); Inhalational Anesthetics; MAC; 7-Nitro Indazole (7-NI); Dexmedetomidine

"Effects of 7-Nitro Indazole on Halothane Minimum Alveolar Anesthetic Concentration"Methods : Fourteen Sprague-Dawley rats were divided into 7-Nitro indazole (7-NI ; selective neuronal nitric oxide synthase inhibitor) group and control group. We measured minimum alveolar anesthetic concentration (MAC) of halothane before and after injection of 7-NI (100 mg/kg) or vehicle. Using NADPH-diaphorase histochemistry, we also investigated the nNOS activity of the locus ceruleus (LC) and the spinal cord (SC).Results : 7-NI reduced the MAC about 50 %, which was accompanied by about 25 % suppression of nNOS staining in SC and LC."Halothane Minimum Alveolar Anesthetic Concentration and NADPH-diaphorase histochemistry after Dexmedetomidine injection"Methods : Thirteen-six Sprague-Dawley rats were divided into three Dexmedetomidine (DEX) groups (50μg/kg bolus, 50μg/kg/day ; three-day and two-week continuous administration) and three control groups (vehicle). We investigated halothane MAC and the nNOS activity of the LC and the SC using NADPH-diaphorase histochemistry.Results : Bolus injection of DEX reduced the MAC about 50 %, however, did not change the NADPH-diaphorase histochemistry. On the other hand, continuous administration of DEX did not reduce the MAC, however, nNOS activity of the LC and IV-V laminae of the lumbar SC decreased in the three-day DEX group, but not the two-week DEX group.Conclusion : Nitric oxide (NO) plays an important role in decision of MAC, however, NO is not only one factor.

“7-硝基吲唑对氟烷最低肺泡麻醉浓度的影响“方法：14只SD大鼠分为7-硝基吲唑（7-NI ;选择性神经元型一氧化氮合酶抑制剂）组和对照组。在注射7-NI（100 mg/kg）或溶媒前后，测定氟烷的最低肺泡麻醉浓度（MAC）。结果：7-NI使脊髓蓝斑（LC）和蓝斑（LC）的MAC降低约50%，同时伴有nNOS染色抑制约25%。方法：将13只SD大鼠随机分为3组（DEX组：50μg/kg推注，50 μ g/kg/d，连续给药3天和2周）和3个对照组（溶媒组）。结果：DEX使LC和SC的MAC降低约50%，但不改变NADPH-黄递酶组织化学染色。结论：一氧化氮（NO）在决定MAC中起重要作用，但NO不是决定MAC的唯一因素。