Effect of Transformation of Plants on Protein Expression

植物转化对蛋白质表达的影响

• 批准号: 10680141
• 负责人: MURATA Masatsune
• 金额: $ 1.86万
• 依托单位: Ochanomizu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680141/
• 关键词: transgenic plant; protein expression; Agrobacterium; tobacco; apple; phenolic; tobacco

One of the issues on genetically modified foods is that unexpected or unintended proteins or metabolites will occur as a result of the genetic modification of crops. Here we have prepared transgenic tobacco and apple shoots as model plants and analyze the proteins and phenolics to give a fundamental knowledge on the safety of the genetically modified foods An antibiotic-resistant gene was used as a maker for the selection of transgenic plants. Beta-glucuronidase gene was used to colorimetrically examine the expression of inserted gene. Proteins and phenolics of transgenic shoots were compared with those of non-transgenic shoots. Proteins and phenolics were analyzed by SDS-PAGE and HPLC, respectively. Consequently, there were no significant differences between them.

转基因食品的一个问题是，农作物的转基因会产生意想不到或非预期的蛋白质或代谢产物。本文以转基因烟草和苹果为模式植物，通过对转基因烟草和苹果的蛋白质和酚类物质的分析，为转基因食品的安全性提供了基础知识。β-葡萄糖醛酸酶基因用于比色法检测插入基因的表达。蛋白质和酚类物质的转基因芽与非转基因芽进行了比较。蛋白质和酚类物质分别通过SDS-PAGE和HPLC分析。因此，它们之间没有显著差异。

项目成果

Masatsune Murata 他: "A Transgenic Apple Callus Showing Reduced Polyphenol Oxidase Activity and Lower Browning Potential"Bioscience, Biotechnology, and Biochemistry. 62(In press). (2001)

Masatsune Murata 等人：“显示多酚氧化酶活性降低和褐变潜力降低的转基因苹果愈伤组织”《生物科学、生物技术和生物化学》62（出版中）。

Masatsune Murata, Makiyo Nishimura, Nanae Murai, Miyoshi Haruta, Seiichi Homma, and Yuji Itoh: "A Transgenic Apple Callus Showing Reduced Polyphenol Oxidase Activity and Lower Browning Potential."Bioscience, Biotechnology, and Biochemistry. 65(In press).

Masatsune Murata、Makiyo Nishimura、Nanae Murai、Miyoshi Haruta、Seiichi Homma 和 Yuji Itoh：“转基因苹果愈伤组织表现出多酚氧化酶活性降低和褐变潜力降低”。生物科学、生物技术和生物化学。

Masatsune Murata 他: "Transgenic Apple (Malus x domestica) Shoot Showing Low Browning Potential"Journal of Agricultural and Food Chemistry,. 48. 5243-5248 (2000)

Masatsune Murata 等人：“转基因苹果（Malus x Domestica）芽显示低褐变潜力”《农业与食品化学杂志》，48. 5243-5248（2000）

Masatsune Murata 他: "Transgenic Apple (Malus x domestica) Shoot Showing Low Browning Potential"Journal of Agricurtural and Food Chemistry,. 48. 5243-5248 (2000)

Masatsune Murata 等人：“转基因苹果（Malus x Domestica）芽显示低褐变潜力”《农业与食品化学杂志》，48. 5243-5248（2000）

Masatsune Murata, Miyoshi Haruta, Nanae Murai, Natsu Tanigawa, Makiyo Nishimura, Seiichi Homma and Yuji Itoh: "Transgenic Apple (Malus x domestica) Shoot Showing Low Browning Potential"Journal of Agricultural and Food Chemistry. 48. 5243-5248 (2000)

Masatsune Murata、Miyoshi Haruta、Nanae Murai、Natsu Tanikawa、Makiyo Nishimura、Seiichi Homma 和 Yuji Itoh：“转基因苹果（Malus x Domestica）芽表现出低褐变潜力”农业和食品化学杂志。