ショウジヨウバエの染色体分配に必須な新規遺伝子orbitの機能解析

果蝇染色体分离必需的新基因轨道的功能分析

• 批准号: 11143216
• 负责人: 井上 喜博
• 金额: $ 1.22万
• 依托单位: Aichi Cancer Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11143216/
• 关键词: 細胞分裂; 染色体分配; 微小管; ショウジョウバエ

ショウジョウバエの新しい細胞分裂突然変異体orbitの細胞では、単極紡錘体微小管の形成による染色体分配の阻害と細胞質分裂の欠損との両方の要因により染色体の倍数化がおこると考えられる。この遺伝子産物は、170KDaの新規タンパクである。その塩基性ドメイン内にβチューブリンやFtsZのGTP結合モチーフに一致した配列が認められる。このタンパクは、M期を通じて微小管上に局在する。Orbitタンパクは、ショウジョウバエの初期胚から調整した抽出液中で微小管と共沈する。この会合は、既知の微小管会合タンパク(MAP)のそれに匹敵するほど強い。さらに大腸菌内で合成させ精製した組み換え体Orbitタンパクは、他のMAPを介さずに微小管に直接結合することを示した。この際、結合にはGTPの加水分解が必要であることが明らかになった。Orbitタンパクは、染色体分配に必須なMAPであると結論できる。中心体の分離および分裂極の伸長に必要なキネシン様モーターKLP61Fの欠損変異体がorbit変異体とよく似た表現型を示すこと、両者の間で強い遺伝学的相互作用が観察されたことから、Orbitタンパクはキネシン様モーターが微小管上で働く際に重要な役割を担うと予想している。hypomorph型変異orbit^1ホモは、雄、雌ともに不妊である。変異体雄の精巣内で多倍体の精原細胞が観察され、さらに雄の減数分裂でも同様に染色体分配および細胞質分裂の両方の欠損を認めた。また変異体雌の卵室内でも少なくとも減数分裂前分裂の異常が観察された。したがってこの遺伝子は体細胞のみならず、生殖細胞形成の時の減数分裂前分裂と減数分裂でも必須なことがわかった。

Because of the abrupt cell division, orbit, microtubule formation, chromosome distribution, cell division, chromosome doubling, chromosome doubling, cell division, chromosome division, cell division, chromosome division, cell division, cell division, chromosome division, cell division, cell division, chromosome division, cell division, cell division, chromosome division, cell division, cell division, chromosome division, chromosome division, cell division, chromosome division, chromosome division, cell division, chromosome division, cell division, chromosome division, chromosome division, cell division, chromosome division, cell division, chromosome division, chromosome division, cell division, chromosome division, chromosome division, cell division, chromosome division, chromosome division, cell division, chromosome division Please do not know what you are going to do, and you will need to know about 170KDa's new regulations. In the basic information system, there is no significant difference between the two groups. The combination of the β-FtsZ GTP and the basic information is consistent with each other. In the first place, the microtubule will be in place during the M phase. Orbit microtubule co-sedimentation in the extraction solution of the early embryo. Rendezvous, we know that microtubule rendezvous (MAP) will match the strength of the rendezvous. The microtubule was directly combined with the microtubule in the microtubule, which was directly combined with the microtubule, the synthetic sperm was synthesized in the bacteria, and the Orbit and MAP were introduced in this paper. It is necessary to make sure that the water is decomposed in combination with GTP. Orbit chromosome distribution and chromosome distribution must be checked by MAP. The centrosome separation is very important. It is necessary to make sure that the KLP61F is not as good as that of the orbit. The type of the table shows that the interaction between the microtubule and the microtubule is very important for the observation of the interaction between the microtubule. Hypomorphs, male and female, do not give birth to pregnancy. The spermatogonia were polyploid, the spermatogonia were observed, the males were divided, the chromosomes were assigned, the cells were divided, and the spermatogonia were not recognized. The body of the female egg is in the room, and the premitotic division is often observed. It is necessary to have a mitosis before meiosis at the time of the formation of reproductive cells.

项目成果

Yamaguchi M.et al.,: "Ectopic expression of human p53 inhibits entry into S phase and induce apoptosis in Drosophila eye imaginal disc"Oncogene. 18. 6767-6775 (1999)

Yamaguchi M.等人：“人 p53 的异位表达抑制果蝇眼成虫盘进入 S 期并诱导细胞凋亡”癌基因。

Inoue Y.H.et al.,: "Orbit, a novel microtubule-associated protein essential for mitosis in Drosophila"J. Cell Biol. (in press).

Inoue Y.H.等人，：“Orbit，一种对果蝇有丝分裂至关重要的新型微管相关蛋白”J.