Molecular analysis for the regulation of gastric cancer cell proliferation regarding with arachidonic acid cascade and reactive oxygen species.

花生四烯酸级联和活性氧对胃癌细胞增殖调节的分子分析。

• 批准号: 11670526
• 负责人: ARAKAWA Tetsuo
• 金额: $ 2.24万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670526/
• 关键词: arachidonic acid cascade; cell proliferation; intracellular signal transduction; cyclooxygenase; cytokine; 大腸癌細胞; アデノウイルス; MAP kinase; 遺伝子治療; AP-1; 胃癌細胞; アポトーシス; アデノウィルス; PPAR; バレット腺癌; 細胞周期; ODC; プロスタグランジン; 転写因子; 炎症性サイトカイン

We investigated the role of the activation of intraceUular signal transduction pathway (MAP kinases) and arachidonic acid cascade stimulated by various growth factors and cytokines such as hepatocyte growth factor, epidemnal growth factor, interieukin-1 beta, or tumor necrosis factor in the proliferation of normal and cancerous gastric epitherilal cells. In addition, regarding with reactive oxygen species, we investigated the correlation of myeloperoxidase activity with gastric ephherila cell proliferation. It was demonstrated that intracellular signal transduction such as MAP kinase pathway and arachidonic acid cascade including prostaglandins played important roles in the regulation of proliferation of gastric cancer cells. Furthermore, myeloperoxidase activity caused by neutrophil infiltration was implicated in not only inflammation but also proliferation of gastric epimerial cells. These detailed analyses should be one of the molecular target for the prevention of gastric cancer growth. We have reported these findings in the specialized international journals as described in the below references. It was thought that all these findings we got in a series of the basic research shewed the original and useful knowledge to the field of gastroenterology.

我们研究了细胞内信号转导通路（MAP激酶）和花生四烯酸级联反应在正常和癌变胃上皮细胞增殖中的作用。此外，在活性氧方面，我们研究了髓过氧化物酶活性与胃上皮细胞增殖的相关性。研究表明，包括拟南芥素在内的细胞内信号转导通路如MAP激酶通路和花生四烯酸级联反应在胃癌细胞增殖调控中起重要作用。此外，中性粒细胞浸润引起的髓过氧化物酶活性不仅与炎症有关，而且与胃上皮细胞的增殖有关。这些详细的分析可能是预防胃癌生长的分子靶点之一。我们在专业国际期刊上报告了这些发现，如以下参考文献所述。认为这些基础研究的发现为消化病学领域提供了新的、有用的知识。

项目成果

Takashima T et al.: "PPAR-gamma ligande inhibit growth of human esophageal adenocarcinoma cells through induction of apoptosis cell cycle arrest and reduction of orrvthine decarb activity"Int J Oncol. 19(3). 465-471 (2001)

Takashima T 等人：“PPAR-gamma 配体通过诱导细胞凋亡、细胞周期停滞和降低 orrvthine decarb 活性来抑制人食管腺癌细胞的生长”Int J Oncol。

Jones MK, et al: "HGF triggers actactivation of the COX-2 gene in rat gostin epithclcal cells action mediated through the ERK2 signaling pathway"FASEB J.. 13. 2186-2194 (1999)

Jones MK 等人：“HGF 通过 ERK2 信号传导途径介导大鼠 gostin 上皮细胞作用中 COX-2 基因的激活”FASEB J.. 13. 2186-2194 (1999)

Tominaga et al.: "Expression of gene for E III A-and E IV B-fibronectin, fetal types of fibronectin during geotric, uncer healing in rats"Dig.Dis.Sci.. 46(2). 311-317 (2001)

Tominaga 等人：“大鼠子宫肌瘤愈合过程中 E III A-和 E IV B-纤连蛋白、胎儿型纤连蛋白基因的表达”Dig.Dis.Sci. 46(2)。

Tomi**aga K et al: "Increased mitogen-Activated Pretein Kinase Activities Srimulated with Ineerle*kin-1-Bata and mechanism(s) of the kinase sigaling Oatheays in Rat Gastric Epithelial Cells"Digestion. 61. 30-38 (2000)

Tomi**aga K 等人：“用 Ineerle*kin-1-Bata 刺激有丝分裂原激活的蛋白质激酶活性，以及​​大鼠胃上皮细胞中激酶信号 Oatheays 的机制”消化。

Tominaga et al.: "In vivo action of novel alkyl methyl quinolone alkaloids against Helicobacter pylori"J Antimicrob Chemother. 50(4). 547-552 (2002)

Tominaga 等人：“新型烷基甲基喹诺酮生物碱对幽门螺杆菌的体内作用”J Antimicrob Chemother。