Functional role of the spiral ligament related to the conchiear recovery

螺旋韧带与贝壳恢复相关的功能作用

• 批准号: 11671683
• 负责人: YAMASHITA Hiroshi
• 金额: $ 2.43万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671683/
• 关键词: inner ear; spiral ligament; plasticity; regeneration; FGF; drug delivery system

1. Cell proliferation in spiral ligament of mouse cochlea damaged by dihydrostreptomycin sulfateMany BrdU labeled nuclei in the spiral ligament in the conchlea damaged by dyhydrostreptomycin sulfate were found. The expressions of fibroblast growth factor receptor and connexin 43 were detected in the spiral ligament where BrdU labeled cells were found. These results suggest that the cell proliferation in the spiral ligament may occur after the drug-induced damage, and this process is probably related to the recovery of the cochlear function.2. Effectiveness and utility of chemical labyrinthectomy with streptomycin sulfate using osmotic pumpLabyrinthectomy was performed surgically or chemically with 30% streptomycin sulfate using osmotic pumps in guinea pigs. After treatment, no statistical differnce was observed in the frequency of spontaneous nystagmus and the degree of yaw head tilt between the groups. These data indicate the reliability and efficacy of osmotic pumps, and it might be possible to make guinea pig models using osmotic pumps to predict various grades of damage in the vestibular periphery of humans.3. The role of acidic fibroblast growth factor in recovery of acoustic traumaWe assessed auditory brain stem response (ABR) thresholds to evaluate cochlear function and observed the sensory epithelium using confocal laser-scanning microscopy. After noise exposure (120dB SPL, 5h), the ABR threshold showed an increase of 〜50dB SPL that recovered after 14 days. Cochlear function in aFGF treated ears recovered more quickly than that in control ears. These results suggest that aFGF may play an important role in cochlear recovery after acoustic trauma.

1.硫酸双氢链霉素损伤小鼠耳蜗螺旋韧带细胞增殖实验结果显示，硫酸双氢链霉素损伤后的耳蜗螺旋韧带内有大量BrdU标记的细胞核，并有成纤维细胞生长因子受体（FGF）和连接蛋白43（Cx43）的表达，提示药物损伤后螺旋韧带内可能发生细胞增殖，这一过程可能与耳蜗功能的恢复有关.应用渗透泵对豚鼠进行硫酸链霉素化学性膀胱切除术的有效性和实用性实验室用渗透泵对30%硫酸链霉素进行了手术或化学性膀胱切除术。治疗后两组患者自发性眼震发生率及偏头程度差异无统计学意义。这些数据表明渗透泵的可靠性和有效性，并有可能使豚鼠模型使用渗透泵来预测不同程度的损害在人类前庭周边.酸性成纤维细胞生长因子在听性损伤恢复中的作用我们用听性脑干反应（ABR）阈值评价耳蜗功能，并用激光共聚焦显微镜观察耳蜗感觉上皮。120dBSPL噪声暴露5h后，ABR阈值升高约50dBSPL，14d后恢复正常。aFGF处理耳的耳蜗功能恢复比对照耳快。这些结果提示aFGF可能在声损伤后耳蜗恢复中起重要作用。

项目成果

Yamashita H,et al.: "Cell proliferation in spiral ligament of mouse cochlea damaged by dihydrostreptomycin sulfate"Acta Otolaryngol (Stockh). 119. 322-325 (1999)

Yamashita H 等人：“硫酸二氢链霉素损伤的小鼠耳蜗螺旋韧带中的细胞增殖”Acta Otolaryngol (Stockh)。

Sugahara K: "The role of acidic fibroblast growth factor in recovery of acoustic trauma"NeuroReport. 12. 3299-3302 (2001)

Sugahara K：“酸性成纤维细胞生长因子在声损伤恢复中的作用”NeuroReport。

Yamashita H, et al.: "Cell proliferation in spiral ligament of mouse cochlea damaged by dihydrostreptomycin sulfate"Acta Otolaryngol (Stockh). 119. 322-325 (1999)

Yamashita H 等人：“硫酸二氢链霉素损伤的小鼠耳蜗螺旋韧带中的细胞增殖”Acta Otolaryngol (Stockh)。

山下 裕司: "内耳の可塑性について"耳鼻咽喉科・頭頭部外科. 73. 9-13 (2001)

Yuji Yamashita：“论内耳的可塑性”耳鼻喉科/头颅外科。 73. 9-13 (2001)。

山下 裕司: "内耳への直接的薬物治療"JOHNS. 17. 57-60 (2001)

Yuji Yamashita：“内耳直接药物治疗”JOHNS. 17. 57-60 (2001)