A gold-coated magnetic nanoparticle biosensor for detecting microRNA

一种用于检测 microRNA 的金涂层磁性纳米粒子生物传感器

• 批准号: LP150101014
• 负责人: Prof John Gooding
• 金额: $ 28.52万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-11-01 至 2020-05-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP150101014
• 关键词: gold; coated; magnetic; nanoparticle; biosensor

The project aims to develop a biosensor for detecting short sequences of RNA, called microRNA (miRNA) in blood. There are about 100 miRNA sequences that are involved in most biological processes. Changes in the levels of some miRNA sequences can serve as a biomarker for many diseases including cancers. The miRNA will be detected using gold-coated magnetic nanoparticles modified with DNA sequences complementary to the miRNA of interest to capture the miRNA. Application of a magnetic field allows the levels of miRNA to be detected electrochemically. The expected outcome is a commercialisable biosensor for miRNA both as a diagnostic early detection device and a prognostic device for a range of miRNA biomarkers.

该项目旨在开发一种生物传感器，用于检测血液中的短RNA序列，称为微RNA(MiRNA)。大约有100个miRNA序列参与了大多数生物过程。一些miRNA序列水平的变化可以作为包括癌症在内的许多疾病的生物标记物。将使用与目标miRNA互补的DNA序列修饰的镀金磁性纳米颗粒来检测miRNA，以捕获miRNA。施加磁场可以用电化学方法检测miRNA的水平。预期的结果是一种可商业化的miRNA生物传感器，既可以作为一种诊断早期检测装置，也可以作为一系列miRNA生物标志物的预测装置。