How electric fields can facilitate reversible protein binding to surfaces

电场如何促进可逆蛋白质与表面的结合

• 批准号: DP220103024
• 负责人: Prof John Gooding
• 金额: $ 31.24万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2022
• 资助国家: 澳大利亚
• 起止时间: 2022-01-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP220103024
• 关键词: How; electric; fields; facilitate; reversible

The aim of this project is to develop the first biosensors that prevent nonspecific protein adsorption and allow reversible protein binding. The project expects to achieve this using a combination of novel surface chemistry and pulsed electric fields that dynamically change a sensing interface. The impact of electric fields on the binding of proteins to this interface will be followed using a novel single molecule fluorescence microscope previously developed that can locate the position of proteins with 2 nanometer resolution. The expected outcomes of this project is a class of biosensor that can continuously monitor protein biomarkers for wearable sensors that provide information on a user’s wellness and nutrition.

该项目的目的是开发第一个生物传感器，防止非特异性蛋白质吸附，并允许可逆蛋白质结合。该项目希望利用新的表面化学和动态改变传感界面的脉冲电场的组合来实现这一点。电场对蛋白质与该界面结合的影响将使用先前开发的新型单分子荧光显微镜进行跟踪，该显微镜可以以2纳米的分辨率定位蛋白质的位置。该项目的预期成果是一种生物传感器，可以连续监测可穿戴传感器的蛋白质生物标记物，提供用户健康和营养的信息。