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Role of Chemokines in the Pathogenesis of Bleomycin-induced Scleroderma

趋化因子在博来霉素诱导的硬皮病发病机制中的作用

基本信息

批准号：
12670810
负责人：
YAMAMOTO Toshiyuki
金额：
$ 2.3万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670810/
关键词：
scleroderma chemokine MCP-1 Bleomycin 動物モデルサイトカイン線維芽細胞

项目摘要

Recent findings suggest the involvement of chemokines in the fibrotic process. We reported that MCP-1 upregulates mRNA expression of not only collagen, but also collagenase and tissue inhibitor of metalloproteinase-1 (TIMP-1) (J Immunol 2000; 164: 6174-6179). MCP-1 was strongly detected in the lesional skin of systemic sclerosis (SSc) (J Dermatol Sci 2001; 26: 133-139), and spontaneous mRNA expression of MCP-1 was also elevated in scleroderma fibroblasts (Eur J Immunol 2001; 31: 2936-2941). We previously established a mouse model for scleroderma by repeated injections of bleomycin, mimicking human scleroderma both histologically and biochemically (J Invest Dermatol 1999; 112: 456-462, J Rheumatol 1999; 26: 2628-2634, Arch Dermatol Res 2000; 292: 535-541). In this model, expression of MCP-1 as well as its receptor, CCR-2, was enhanced in the lesional skin following bleomycin treatment, in vivo neutralization with anti-MCP-1 antibody together with local bleomycin treatment reduced the development of dermal sclerosis. In vitro data demonstrated that MCP-1 upregulates mRNA expression of extracellular matrix proteins. These data suggest MCP-1/CCR-2 pathway plays an important role in the pathogenesis of bleomycin-induced scleroderma.

最近的研究结果表明，在纤维化过程中的趋化因子的参与。我们报道了MCP-1不仅上调胶原蛋白的mRNA表达，而且上调胶原酶和金属蛋白酶组织抑制剂-1（TIMP-1）的mRNA表达（J Immunol 2000; 164：6174-6179）。在系统性硬化症（SSc）的病变皮肤中强烈检测到MCP-1（J Dermatol Sci 2001; 26：133-139），并且在硬皮病成纤维细胞中MCP-1的自发mRNA表达也升高（Eur J Immunol 2001; 31：2936-2941）。我们先前通过重复注射博来霉素建立了硬皮病的小鼠模型，在组织学和生物化学上模拟人硬皮病（J Invest Dermatol 1999; 112：456-462，J Rheumatol 1999; 26：2628-2634，Arch Dermatol Res 2000; 292：535-541）。在该模型中，MCP-1及其受体CCR-2的表达在博来霉素治疗后的病变皮肤中增强，用抗MCP-1抗体体内中和与局部博来霉素治疗一起减少了真皮硬化的发展。在体外数据表明，MCP-1上调细胞外基质蛋白的mRNA表达。这些数据表明MCP-1/CCR-2通路在博莱霉素诱导的硬皮病的发病机制中起重要作用。