Reconstruction of human autologous skin using three dimensional culture and its clinical application

点击翻译按钮获取中文摘要

We have been performing basic and clinical research on the in vitro reconstruction of autologous skin using a novel three dimensional culture system in order to establish the treatment for intractable skin ulcers. In the previous study, we established the in vitro method to generate both keratinocytes and fibroblasts form the identical donor and could reconstruct the skin structure composed of those two cell types in the three dimensional culture system.In the present study, we further investigated the mRNA expressions of collagen 1A2 and 3, matrix metalloproteinase (MMP) 1 and tissue inhibitor of MMP-1 under hypoxic condition that is one of causative factors in intractable skin ulcers. We confirmed the significant decrease in mRNA expressions of collagen 1A2 and 3 as well as the significant increase in mRNA expressions of MMP-1 and those changes were restored by reoxygenation. These findings strongly suggest that hypoxic condition, usually found in a poor blood supply, may prolong or suppress the wound healing. In the clinical study using a thermography, we disclosed that a new type of nitroglycerine tape was effective to improve the peripheral circulatory disturbance in patients with systemic sclerosis, a representative disease, complicated with skin ulcers resistant to the conventional therapies.