Evaluation of tumor angiogenesis by dynamic MRI and compartment analysis

通过动态 MRI 和区室分析评估肿瘤血管生成

• 批准号: 12670877
• 负责人: SUGAWARA Yoshifumi
• 金额: $ 1.15万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670877/
• 关键词: tumor; angiogenesis; MRI; deconvolution analysis; compartment analysis; Gd-DTPA; 悪性腫瘍; コンパートメント解析; ガドリニウム

Angiogenesis correlates with tumor growth, invasion and metastasis. Although tumor blood flow increased by angiogensis, it has not been established to noninvasively measure tumor blood flow in vivo. In this research project, we have developed a new method for quantitative measurement of tumor blood flow (TBF) in vivo by using dynamic GdDTPA enhanced magnetic resonance imaging (MRI) and compartment analysis. By fundamental phantom studies, we confirmed good linear relationships between MRI signal intensities and Gd-DTPA concentrations. Preliminarily, we measured normal muscle blood flow in volunteers and confirmed that muscle blood flow could be measured with a good reproducibily by deconvolution analysis and compartment analysis using arterial input function after a bolus injection of Gd-DTPA. We applied our methods in patients with bone and soft-tissue tumors and successfully generated a functional map of TBF with high temporal (scan time = 3.2 sec) and spatial resolution (pixel-by-pixel). TBF map clearly demonstrated heterogeneity of TBF, i.e. increased TBF in tumor periphery due to angiogenesis and low TBF in center of tumor. TBF map showed substantially decreased TBF after chemotherapy. Thus, TBF map would be a promising tool for evaluating tumor blood flow and may be useful to evaluate treatment response. It is necessary to evaluate if our methods could be applied to tumor of other regions where dynamic data analysis would be hindered by motion artifact to date. In future studies, it is also warranted to compare the TBF maps with the results of other imaging modalities and histopathologic findings.

血管生成与肿瘤生长、侵袭和转移相关。虽然肿瘤血流量增加血管生成，它还没有建立在体内无创测量肿瘤血流量。在本研究项目中，我们开发了一种新的方法，定量测量肿瘤血流量（TBF）在体内使用动态GdDTPA增强磁共振成像（MRI）和房室分析。通过基础体模研究，我们证实MRI信号强度与Gd-DTPA浓度之间存在良好的线性关系。首先，我们测量了志愿者的正常肌肉血流量，并证实了在团注Gd-DTPA后，使用动脉输入函数的去卷积分析和房室分析可以测量肌肉血流量，具有良好的重现性。我们将我们的方法应用于骨和软组织肿瘤患者，并成功地生成了具有高时间（扫描时间= 3.2秒）和空间分辨率（逐像素）的TBF功能图。TBF图清楚地显示了TBF的异质性，即肿瘤周围由于血管生成而增加的TBF和肿瘤中心的低TBF。TBF图显示化疗后TBF明显降低。因此，TBF图将是一个有前途的工具，用于评估肿瘤血流，并可能是有用的，以评估治疗反应。有必要评估我们的方法是否可以应用于其他区域的肿瘤，其中动态数据分析会受到运动伪影的阻碍。在未来的研究中，还需要将TBF图与其他成像方式和组织病理学结果进行比较。

项目成果

Kikuchi K, et al.: "Measurement of cerebral hemodynamics with perfusion-weighted MR imaging : comparison with pre-and post-acetazolamide 133Xe-SPECT in occlusive carotid disease"AJNR Am J Neuroradiol. 22. 248-254 (2001)

Kikuchi K 等人：“用灌注加权 MR 成像测量脑血流动力学：与闭塞性颈动脉疾病中乙酰唑酰胺 133Xe-SPECT 前后比较”AJNR Am J Neuroradiol。

Kikuchi T, et al.: "Quantitative evaluation of mean transit time obtained with dynamic susceptability contrast-enhanced MR imaging : compared to Xe-133 single photon emission CT in occlusive cerebrovascular disease"JR Am J Roentgenol. (in press).

Kikuchi T 等人：“通过动态磁化率对比增强 MR 成像获得的平均通过时间的定量评估：与闭塞性脑血管疾病中的 Xe-133 单光子发射 CT 相比”JR Am J Roentgenol。

Kikuchi T., Sugawara Y., Kajihara M., et al.: "Accumulation of Tc-99m HMDP in ovarian serous papillary adenocarcmoma reflecting ongoing calcification"Clin Nucl Med. 26. 886-887 (2001)

Kikuchi T.、Sugara Y.、Kajihara M. 等人：“卵巢浆液性乳头状腺癌中 Tc-99m HMDP 的积累反映了正在进行的钙化”Clin Nucl Med。

Kikuchi T, et al.: "Accumulation of Tc-99m HMDP in ovarian serous papillary adenocarcinoma reflecting ongoing calcification"Clin Nucl Med. 26. 886-887 (2001)

Kikuchi T 等人：“卵巢浆液性乳头状腺癌中 Tc-99m HMDP 的积累反映了正在进行的钙化”Clin Nucl Med。

Sugawara Y., Ueda T., Kikuchi T., et al.: "Hyperactivity of 99mTc-HMPAO within 6 hours in patients with acute ischemic stroke"J Nucl Med. 42. 1297-1302 (2001)

Sukawara Y.、Ueda T.、Kikuchi T. 等人：“急性缺血性中风患者 6 小时内 99mTc-HMPAO 的过度活性”J Nucl Med。