In vitro induction of pancreatic β-cell development from mouse embryonic stem cells

小鼠胚胎干细胞体外诱导胰腺 β 细胞发育

基本信息

  • 批准号:
    12671113
  • 负责人:
  • 金额:
    $ 2.5万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

Embryonic stem cells (ES cells) are pluripotent cells derived from the inner cell mass of fertilized blastocysts. ES cells display the ability to differentiate in vitro into a variety of cell lineages. It has been shown that ES cells can be differentiated to hematopoietic cells, neurons and cardiomyocytes. We investigate the possibility to differentiate the ES cells to insulin secreting pancreatic β-cells. In order to facilitate the differentiation to the insulin producing cells, we manipulated ES cells to stably express IPF-1, a transcription factor which plays critical roles in the development of pancreas and pancreatic β-cells. After production of embryoid bodies, IPF-1 producing ES cells were rendered to differentiate in the serum free medium of Lumelsky et al (Science 292: 1389) with modification. IPF-1 producing ES cell-derived cells produced up to 5 times more immunoreactive insulin when compared with the differentiated cells derived from native ES cells. We confirmed the expression of immunoreactive insulin in approximately 30 % of the cells at final differentiation stage by immunohistochemical analysis. We are currently trying to optimize the condition for more efficient differentiation. At the same time, we have elaborated the ES cells marked by EGFP in which EGFP can be expressed specifically in the insulin-producing cells. Upon the differentiation, insulin secreting cells will be able to be selected by fluorescence-assisted cell sorting using these cells.
胚胎干细胞(Embryonic stem cells,ES细胞)是从受精胚泡的内细胞团中分离出来的多能性细胞。ES细胞显示出在体外分化成多种细胞谱系的能力。研究表明,ES细胞可以分化为造血细胞、神经元和心肌细胞。我们研究了ES细胞分化为胰岛素分泌胰腺β细胞的可能性。为了促进向胰岛素产生细胞的分化,我们操纵ES细胞以稳定表达IPF-1,IPF-1是一种在胰腺和胰腺β细胞发育中起关键作用的转录因子。在产生胚状体后,使产生IPF-1的ES细胞在Lumelsky等(Science 292:1389)的无血清培养基中分化,并进行了修改。与天然ES细胞衍生的分化细胞相比,产生IPF-1的ES细胞衍生细胞产生的免疫反应性胰岛素高出5倍。我们通过免疫组织化学分析证实了免疫反应性胰岛素在大约30%的最终分化阶段的细胞中表达。我们目前正在努力优化条件,以实现更有效的分化。同时,我们还对EGFP标记的ES细胞进行了详细的研究,在这些细胞中,EGFP可以在胰岛素分泌细胞中特异性表达。在分化后,将能够通过使用这些细胞的荧光辅助细胞分选来选择胰岛素分泌细胞。

项目成果

期刊论文数量(23)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Tanizawa,Y.: "Genetic analysis of Japanese patients with persistent hyperinsulinemic hypoglycemia of infancy. NBF-2 mutation impairs cooperative binding of adenine nucleotides to SUR1."Diabetes. 49. 114-120 (2000)
Tanizawa,Y.:“对婴儿期持续高胰岛素性低血糖的日本患者进行基因分析。NBF-2 突变损害腺嘌呤核苷酸与 SUR1 的协同结合。”糖尿病。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
井上 寛: "分子糖尿病学の進歩2000(Wolfram(DIDMOAD)症候群の原因遺伝子の同定)"金原出版. 196 (2000)
Hiroshi Inoue:“2000 年分子糖尿病学进展(确定导致 Wolfram (DIDMOAD) 综合征的基因)”Kanehara Publishing 196 (2000)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Tanizawa,Y.: "Positional cloning of Wolfram syndrome gene (WFS1)"Gene and Medicine. 4. 313-317 (2000)
Tanizawa,Y.:“Wolfram 综合征基因 (WFS1) 的定位克隆”基因与医学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Tanizawa Y: "Unregulated elevation of glutamate dehydrogenase activity induces glutamine-stimulated insulin secretion"Diabetes. 51. 712-717 (2002)
Tanizawa Y:“谷氨酸脱氢酶活性不受控制的升高会诱导谷氨酰胺刺激的胰岛素分泌”糖尿病。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Emoto M: "Troglitazone Treatment Increases Plasma vascular endothelial growth factor in diabetic patients and its mRNA in 3T3-L1 Adipocytes."Diabetes. 50. 1166-1170 (2001)
Emoto M:“曲格列酮治疗可增加糖尿病患者的血浆血管内皮生长因子及其在 3T3-L1 脂肪细胞中的 mRNA。”糖尿病。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

TANIZAWA Yukio其他文献

TANIZAWA Yukio的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('TANIZAWA Yukio', 18)}}的其他基金

Peripheral Circadian Dysregulation and Metabolic Disorders
周围昼夜节律失调和代谢紊乱
  • 批准号:
    15H04849
  • 财政年份:
    2015
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Investigation of the mechanism of beta-cell failure in diabetes using Wolfram Syndrome as a model
以 Wolfram 综合征为模型研究糖尿病 β 细胞衰竭的机制
  • 批准号:
    23390080
  • 财政年份:
    2011
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Investigation of the mechanism of pancreatic beta-cell death underlying progressive nature of type 2 diabetes mellitus, aiming at the development of treatment strategy for the disease condition.
研究2型糖尿病进行性的胰腺β细胞死亡机制,旨在制定针对该疾病的治疗策略。
  • 批准号:
    20390093
  • 财政年份:
    2008
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Investigation of insulin resistance-induced endoplasmic reticulum stress in the pancreatic β-cell and development of diabetes mellitus
胰岛素抵抗诱导的胰腺β细胞内质网应激与糖尿病发生的研究
  • 批准号:
    18390103
  • 财政年份:
    2006
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular Pathophysiology of Wolfram Syndrome and Endoplasmic Reticulum Stress-associated Pancreatic β-cell Failure.
Wolfram 综合征和内质网应激相关胰腺 β 细胞衰竭的分子病理生理学。
  • 批准号:
    16390096
  • 财政年份:
    2004
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Investigation of the roles of WFS1 and glutamate dehydorogenase on the pancreatic β-cell function and regulation of insulin secretion
WFS1和谷氨酸脱氢酶对胰腺β细胞功能和胰岛素分泌调节作用的研究
  • 批准号:
    14370338
  • 财政年份:
    2002
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Elucidation of molecular pathophysiology and development of the methods for molecular diagnosis of the disease associated with WFS1 gene mutations.
阐明分子病理生理学并开发与 WFS1 基因突变相关疾病的分子诊断方法。
  • 批准号:
    11557012
  • 财政年份:
    1999
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Regulation of Pacreatic β-cell Function by Transcription Factors.
转录因子对胰腺 β 细胞功能的调节。
  • 批准号:
    10671074
  • 财政年份:
    1998
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

The Role of Pancreatic β-cell Store-Operated Calcium Entry in Fatty Acid Metabolism
胰腺β细胞库操作的钙进入在脂肪酸代谢中的作用
  • 批准号:
    9910840
  • 财政年份:
    2019
  • 资助金额:
    $ 2.5万
  • 项目类别:
Molecular characterization of Hunk, a novel regulator of pancreatic β-cell function
Hunk 的分子表征,一种新型胰腺β细胞功能调节剂
  • 批准号:
    419280124
  • 财政年份:
    2018
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Research Fellowships
The impact of platelets on the maintenance of pancreatic β cell mass and development of diabetes (B10+)
血小板对维持胰腺β细胞质量和糖尿病发展的影响(B10)
  • 批准号:
    406534138
  • 财政年份:
    2018
  • 资助金额:
    $ 2.5万
  • 项目类别:
    CRC/Transregios
Development of a rapid and inexpensive luciferase-based high throughput screening assay to identify compounds that alter pancreatic β cell function
开发快速且廉价的基于荧光素酶的高通量筛选测定法,以识别改变胰腺β细胞功能的化合物
  • 批准号:
    9254620
  • 财政年份:
    2016
  • 资助金额:
    $ 2.5万
  • 项目类别:
IL-22 as a Suppressor of Pancreatic β-Cell Stress and a Treatment for Diabetes
IL-22 作为胰腺β细胞应激的抑制剂和糖尿病的治疗
  • 批准号:
    nhmrc : GNT1081473
  • 财政年份:
    2015
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Project Grants
Analysis about the factors that affect the postnatal formationand function of pancreatic β-cell in connection with the intrauterine undernutrition.
宫内营养不良影响出生后胰岛β细胞形成和功能的因素分析
  • 批准号:
    22590976
  • 财政年份:
    2010
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation of roles of GSK-3β in the regulation of pancreatic β cell mass for the application to the treatment of type 2 diabetes mellitus.
研究 GSK-3β 在调节胰腺 β 细胞质量中的作用及其在 2 型糖尿病治疗中的应用。
  • 批准号:
    22590984
  • 财政年份:
    2010
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Pancreatic β cell imaging using 64Cu- or 68Ga-labeled Exendin-4
使用 64Cu 或 68Ga 标记的 Exendin-4 进行胰腺 β 细胞成像
  • 批准号:
    21791228
  • 财政年份:
    2009
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
The roles of transcription factor FoxO1 in pancreatic β cell proliferation and neogenesis
转录因子FoxO1在胰腺β细胞增殖和新生中的作用
  • 批准号:
    21790862
  • 财政年份:
    2009
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Effect of intrauterine undernutrition during late gestation on pancreatic β cell mass
妊娠晚期宫内营养不良对胰腺β细胞量的影响
  • 批准号:
    19591052
  • 财政年份:
    2007
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了