Exploring new candidate genes for pulpal mineralization using micro array system

点击翻译按钮获取中文摘要

The purpose of this study is to find out new candidates that are responsible to mineralization, and to determine their expression in the dental pulp in order to establish a new method of hard tissue formation in the pulp. First, gene expression profiles were compared between Kusa A and Kusa.O cells, which are matured and immatured osteoblastic cell lines respectively. Next, gene expression profiles were compared between Kusa cells cultured in calcifying condition and in normal condition, that are presence of ascorbic acid (AA) and b-glycerophosphate (bGP) or absence of them respectively. Kusa A and O cells are derived from mouse stromal cells, and both cell lines exhibit high alkaline phosphatase activity. Kusa A cells showed in vivo calcification using diffusion chamber transfer into the mice peritoneal, but Kusa O cells did not. Furthermore, Kusa A demonstrated osteocalcin gene expression and in vitro mineralization, therefore Kusa A thought, to be highly differentiated osteoblasts. On the other hand, Kusa 0 cells thought to be immature osteoblasts. Gene expression profile were compared between Kusa A and O cells, and several genes were up regulated and dowirregulated. AA and bGP rapidly depressed the many gene expression. Originally used CDNA array were plotted only 588 genes, and further studies were performed with the Mouse GEM CDNA array (Kurabo) in which 8700 clones were plotted. Several interesting genes were picked, and functional analysis is ongoing.