Analysis of 3-D cultured dental pulp cells and osteoblasts and their clinical application

3D培养牙髓细胞和成骨细胞分析及其临床应用

• 批准号: 22659343
• 负责人: KAWASHIMA Nobuyuki
• 金额: $ 2.07万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659343/
• 关键词: 歯髄細胞; 骨芽細胞; 象牙芽細胞; 細胞分化; 硬組織形成; 三次元培養; 二次元培養; 分化; 石灰化; 低酸素; 未分化; 遺伝子発現解析; 初代培養; 成長因子; 臨床応用

Dental pulp cells are differentiate into odontoblasts, which are dentin-forming cells, and osteoblasts are bone tissue-forming cells. These cells are categorized as hard tissue forming cells, and may possess common properties. Mineralization of these cells in the conventional two dimensional culture is only observed in the presence of mineralization-inducing factors, such as BMPs or ascorbic acid and beta-glycerophosphate. We revealed that three dimensional (3-D) culture induced up-regulation of odonto-/osteo-blast marker expression in dental pulp cells and osteoblasts. Transplantation of 3-D cultured pulp cells or osteoblasts into bone cavities effectively induced hard tissue formation. Clinical application of 3-D cultured dental pulp cells or osteoblasts are expected.

牙髓细胞分化为成牙本质细胞，即牙本质形成细胞，成骨细胞为骨组织形成细胞。这些细胞被归类为硬组织形成细胞，并可能具有共同的特性。在传统的二维培养中，只有在矿化诱导因素存在的情况下，才能观察到这些细胞的矿化，如BMPs或抗坏血酸和β-甘油磷酸。我们发现三维(3-D)培养诱导牙髓细胞和成骨细胞中牙本质/成骨细胞标志物的表达上调。将三维培养的牙髓细胞或成骨细胞移植到骨腔内，有效地诱导了硬组织的形成。三维培养的牙髓细胞或成骨细胞有望在临床上应用。

项目成果

Promotional effects of vasoactive intestinal peptide on the development of rodent Hertwig’s epithelial root sheath

血管活性肠肽对啮齿动物Hertwig上皮根鞘发育的促进作用

• DOI: 10.1111/j.1741-4520.2012.00371.x
• 发表时间: 2012
• 期刊: Congenital Anomalies
• 影响因子: 1.3
• 作者: J Xu;N Kawashima;N Fujiwara;H Harada;MS. Ota;H Suda
• 通讯作者: H Suda

歯髄幹細胞と再生医療

牙髓干细胞与再生医学

• 发表时间: 2011
• 作者: 丸濵功太郎;松香芳三;寺山隆司;窪木拓男;杉本朋貞;福安 翔,江草 宏,萱島浩輝,裏口真也,鎌野優弥,佐伯万騎男,矢谷博文;川島伸之
• 通讯作者: 川島伸之

Effects of MMP-3 on Inflammatory Mediator Synthesis from Macrophages

MMP-3 对巨噬细胞合成炎症介质的影响

• 发表时间: 2010
• 作者: K Takimoto;N Kawashima;M Nakashima;H Nakamura;Y Koizumi;H Suda
• 通讯作者: H Suda

Osteogenic differentiation of mouse mesenchymal progenitor cell, Kusa-A1 is promoted by mammalian transcriptional repressor

哺乳动物转录阻遏蛋白促进小鼠间充质祖细胞 Kusa-A1 成骨分化

• 发表时间: 2010
• 期刊: Rbpj Biochemical and Biophysical Research Communications
• 作者: S. Wang;N. Kawashima;K. Sakamoto;K. Katsube;A. Umezawa;H Suda
• 通讯作者: H Suda

Comparison of Gingiva, Dental Pulp, and Periodontal Ligament Cells From the Standpoint of Mesenchymal Stem Cell Properties

• DOI: 10.3727/215517912x653319
• 发表时间: 2012-04-01
• 期刊: CELL MEDICINE
• 作者: Otabe, Koji;Muneta, Takeshi;Sekiya, Ichiro
• 通讯作者: Sekiya, Ichiro