Studies on dual-functioning of bio-absorbable scaffold materials and cells for the rehabilitation of oral tissues

生物可吸收支架材料与细胞双重功能用于口腔组织康复的研究

• 批准号: 12671880
• 负责人: TAIRA Masayuki
• 金额: $ 2.3万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671880/
• 关键词: tissue engineering; bio-absorbable materials; cell culture; collagen gel; polylactide sponge; bone marrow stromal cells; osteogenic differentiation; gene analysis; コラーゲン; ゼラチン; 足場材料; ポリ乳酸; 遺伝子分析; チタン; アパタイト; アルジネート; 口腔組織; 組織再生

The findings obtained are described in the following four categories.(1) Studies on the preparation of bio-absorbable scaffold materials : We succeeded in producing inter-connected porous sponges by freeze-drying from natural materials (collagen and gelatin) and synthetic materials (alginate and poly(lactide-caprolacton)).(2) Studies on the cell culture : We investigated the effect of medium type (alfa-ME or DME), serum amount, dexamethasone induction and the growth factors (e.g. TGF-beta) on the proliferation and differentiation of rat bone marrow stromal cells and osteoblastic MC3T3-E1 cells. The former cells required the addition of vitamin C and the serum amount of more than 10% for the cell proliferation. The dexamethasone and the growth factors appeared to accelerate the osteogenic differentiation.(3) Studies on the duplex-functioning of the cells and scaffold materials : we successfully cultured the rat bone marrow stromal cells in the collagen gel frame-worked by poly (lactide- caprolacton) sponge. We examined the degree of osteogenic differentiation of rat bone marrow stromal cells cultured in the collagen gel. With the DME medium supplemented with vitamin C, the cells could differentiate into the pre-osteoblasts with their phenotype characterized by the appearance of mRNA for osteopontin. The cells in this stage appear to contribute to the rehabilitation of lost oral hard tissues in the future.(4) Studies on the tissue reaction against the scaffold materials : We implanted prepared scaffold materials (collagen gel, collagen sponge, poly (lactide-caprolacton) sponge, and gelatin hydro-gel) in the back tissue of SD rats, and evaluated the histo-pathological reaction against these scaffold materials. All scaffold materials were mildly absorbed by the host tissue without any strong pathological reaction.

（1）关于制备生物吸收的脚手架材料的研究进行了描述。或DME），血清量，地塞米松诱导和生长因子（例如TGF-β）在大鼠骨髓基质细胞和成骨细胞MC3T3-E1细胞上的增殖和分化上。前者需要添加维生素C，细胞增殖的血清量超过10％。地塞米松和生长因子似乎加速了成骨分化。（3）对细胞和脚手架材料的双链功能的研究：我们成功地培养了由聚（乳糖 - caprolacton）Sponge胶原蛋白凝胶框架中的大鼠骨骨髓细胞。我们检查了在胶原蛋白凝胶中培养的大鼠骨髓基质细胞的成骨分化程度。使用补充维生素C的DME培养基，细胞可以通过其表型分化为骨细胞的表型，其特征是骨桥蛋白的mRNA出现。 The cells in this stage appear to contribute to the rehabilitation of lost oral hard tissues in the future.(4) Studies on the tissue reaction against the scaffold materials : We implanted prepared scaffold materials (collagen gel, collagen sponge, poly (lactide-caprolacton) sponge, and gelatin hydro-gel) in the back tissue of SD rats, and evaluated the histo-pathological reaction against these scaffold 材料。所有脚手架材料都被宿主组织轻度吸收而没有任何强烈的病理反应。

项目成果

TAIRA, M., ARAKI, Y. and TAKAHASHI, J.: "Scaffold consisting of poly(lactide-caprolacton) sponge, collagen gel and bone marrow stromal cells for tissue engineering"J.Mater.Sci.Lett.. 20(19). 470-477 (2001)

TAIRA, M., ARAKI, Y. 和 TAKAHASHI, J.：“用于组织工程的由聚（丙交酯-己内酯）海绵、胶原凝胶和骨髓基质细胞组成的支架”J.Mater.Sci.Lett.. 20(19)

M.Taira,M.S.Toguchi,Y.Hamada,J.Takahashi,R.Itoh,S.Toyosawa,N.Ijyuin and M.Okazaki: "Studies on cytotoxic effect of nickel ions on three cultured fibroblasts"Journal of Materials Science : Materials in Medicine. 12(印刷中). (2001)

M.Taira、M.S.Toguchi、Y.Hamada、J.Takahashi、R.Itoh、S.Toyosawa、N.Ijyuin 和 M.Okazaki：“镍离子对三种培养成纤维细胞的细胞毒性作用的研究”材料科学杂志：材料12（正在出版）（2001）。

M.Taira,M.S.Toguchi,Y.Hamada,M.Okazaki,J.Takahashi,R.Itoh,S.Toyosawa and N.Ijyuin: "Studies on cytotoxicity of nickel ions using C3H10T1/2 fibroblast cells"Journal of Oral Rehabilitation. 27・6. 1068-1072 (2000)

M.Taira、M.S.Toguchi、Y.Hamada、M.Okazaki、J.Takahashi、R.Itoh、S.Toyosawa 和 N.Ijyuin：“使用 C3H10T1/2 成纤维细胞研究镍离子的细胞毒性”口腔康复杂志。 27・6。1068-1072（2000）

TAIRA, M., NAKAO, H., TAKAHASHI, J. and ARAKI, Y.: "Cellular reactions to polylactide-based sponge and collagen gel in subcutaneous tissue"J.Oral Rehabil.. 30(1). 106-109 (2003)

TAIRA, M.、NAKAO, H.、TAKAHASHI, J. 和 ARAKI, Y.：“皮下组织中聚丙交酯海绵和胶原凝胶的细胞反应”J.Oral Rehabil.. 30(1)。

Taira, M., Nakao, H., Takahashi, J., Araki, Y.: "Effects of two vitamins, two growth factors and dexamethasone on the proliferation of rat bone marrow stromal cells and osteoblastic MC3T3-E1 cells"J.Oral Rehabilitation. 30(印刷中). (2003)

Taira, M.、Nakao, H.、Takahashi, J.、Araki, Y.：“两种维生素、两种生长因子和地塞米松对大鼠骨髓基质细胞和成骨细胞 MC3T3-E1 细胞增殖的影响”J.Oral康复。30（正在出版）（2003）。