Evaluation of cell damage and allergy-induced mechanism by minute substance eluted from dental bio-materials

通过牙科生物材料中洗脱的微小物质评估细胞损伤和过敏诱发机制

• 批准号: 15390597
• 负责人: TAIRA Masayuki
• 金额: $ 7.04万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390597/
• 关键词: Nickel ion; Titanium ion; Macrophage; Cell viability; Apoptosis; Superoxide dismutase; Inflammatory cytokine; DNA microarray; リポポリサッチャライド; TNF-α; Niイオン; RAW264; DNA損傷; Ti粉末; 貪食; 活性酸素; ハプテン形成; マウスマクロファージ; NO産生量; PIXE; DNAマイクロアレー; 解毒系蛋白

(1)With increasing nickel ion concentrations, cell viability of macrophage-like RAW264 cells declined, and nitric oxide production of LPS-stimulated RAW 264 cells also declined. DNA microarray analyses showed that nickel ions caused cell cycle arrest, induction of apoptosis, production of inflammatory cytokines (IL-1α、IL-1α、IL-6、TNF) and increased oxidative stress, which lead to cellular damage and systematic body damage such as allergy and cancer.(2)Cell viability of macrophage-like RAW264 cells cultured in medium with 1ppm titanium ions declined to about 55%, compared to that of control cells without titanium ions. SOD activities and TNF-α production of macrophage-like RAW264 cells cultured in medium with 1ppm titanium ions doubled, compared to those of the control cells without titanium ions. It was speculated that phagocytosis of titanium-protein complex by macrophage caused inflammatory cellular reactions.(3)Elution of 10μm titanium particles into distilled water, MEM solution and lactic acid for 15 days was found to be negligible (0), 0.01 ppm and 9 ppm, respectively. Cell viability and NO production of macrophage-like RAW264 cells that phagocytized 10μm titanium particles were 89.4% and 91.6%, respectively. This means that macropage's cellular damage caisued by phagocytosis of 10μm titanium particles was small and tolerable.

（1）随着镍离子浓度的增加，巨噬细胞样的RAW264细胞的细胞活力下降，LPS刺激的RAW 264细胞的一氧化氮产生也下降了。 DNA微阵列分析表明，镍离子引起细胞周期停滞，诱导炎症性细胞因子（IL-1α，IL-1α，IL-1α，IL-6，TNF）的产生，氧化物胁迫增加，导致细胞损伤和系统的身体损伤，例如Allergy和癌症（2）较大的细胞的细胞含量。与没有钛离子的对照细胞相比，为55％。与没有钛离子的对照细胞相比，在具有1ppm钛离子的培养基中培养的巨噬细胞样RAW264细胞的SOD活性和TNF-α产生培养物。据推测，巨噬细胞通过巨噬细胞吞噬钛蛋白复合物引起炎症性细胞反应。（3）发现10μm钛颗粒在蒸馏水中，MEM溶液和乳酸溶解15天被发现可忽略不计（0），0.01 ppm和9 ppm。吞噬10μM钛颗粒的细胞活力和巨噬细胞样的RAW264细胞的产生分别为89.4％和91.6％。这意味着，巨型钛颗粒的吞噬作用造成了宏观的细胞损伤，且可耐受。

项目成果

International Congress Series : International Symposium for Interface Oral Health 1284

国际大会系列：界面口腔健康国际研讨会 1284

• 发表时间: 2005
• 作者: Taira;M.;Sasaki;M.;Kimura;S.;Araki;Y.
• 通讯作者: Y.

Effect of Ni2+ ions on cell viability and NO production of murine periotoneal exudate cells (macrophages) with and without lipopolysaccharide stimulation

Ni2 离子对有和没有脂多糖刺激的小鼠腹膜渗出细胞（巨噬细胞）的细胞活力和 NO 产生的影响

• 发表时间: 2005
• 期刊: Dent.Mater.J. 24・3
• 作者: Taira;M.;Sasaki;M.;Yamaura-Tanaka;C.;Saitoh;S.;Kimura;S.;Araki;Y.
• 通讯作者: Y.

Taira, M., Chosa, N., Sasaki K., Saitoh, S., Sato, N., Takahashi, J., Araki, Y.: "Eeffects of the medium, serum and dexamethasone on the cell proliferation and alkaline phosphatase activity of twice-passaged SD rats' bone marrow stromal cells"Journal of O

Taira, M., Chosa, N., Sasaki K., Saitoh, S., Sato, N., Takahashi, J., Araki, Y.：“培养基、血清和地塞米松对细胞增殖和碱性磷酸酶的影响

Taira, M., Nakao, H., Takahashi, J., Araki, Y.: "Effects of two vitamins, two growth factors and dexamethasone on the proliferation of rat bone marrow stromal cells and osteoblastic MC3T3-E1 cells"J.Oral Rehabil.. 30・7. 697-701 (2003)

Taira, M.、Nakao, H.、Takahashi, J.、Araki, Y.：“两种维生素、两种生长因子和地塞米松对大鼠骨髓基质细胞和成骨细胞 MC3T3-E1 细胞增殖的影响”J.Oral康复..30・701 (2003)

Sulfuration resistance of five experimental Ag-Pd-Au-Cu alloys with low Pd content of 10 or 12%.

硫化%20电阻%20of%205%20实验%20Ag-Pd-Au-Cu%20合金%20与%20low%20Pd%20含量%20of%2010%20或%2012%。

• 发表时间: 2006
• 期刊: Dent.Mater.J. 26(2)(in press)
• 作者: Saitoh;S.;Araki;Y.;Taira;M.
• 通讯作者: M.