Evaluation on clarification of transport system of transition metallic ions inside body-defense cells and its association with bio-safety

我们在含铜离子的培养基中培养巨噬细胞，并通过定量PCR评估基因表达。细胞膜转运蛋白SLC 31 A1基因、内质网转运蛋白ATP 7A/7 B基因和铜转运蛋白伴侣ATOX 1基因的表达均在铜浓度大于200μmol/L时下降。当大量的铜离子没有从巨噬细胞中去除（运输）时，认为细胞内过量的铜离子可能引起DNA损伤（DNA链断裂），导致细胞死亡。

We cultured macrophage in the medium with copper ions, and evaluated gene expression by quantitative PCR. All expressions of cell-membrane transporter SLC31A1 gene, ER transporter ATP7A/7B genes and copper transport chaperon ATOX1 gene declined when the copper content was larger than 200μmol/L. When high amounts of copper ions were not removed(transported) from the macrophage, it was considered that intracellular excessive copper ions might cause DNA damage(DNA-strand breakage), leading to cell death.