Evaluation on clarification of transport system of transition metallic ions inside body-defense cells and its association with bio-safety

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We cultured macrophage in the medium with copper ions, and evaluated gene expression by quantitative PCR. All expressions of cell-membrane transporter SLC31A1 gene, ER transporter ATP7A/7B genes and copper transport chaperon ATOX1 gene declined when the copper content was larger than 200μmol/L. When high amounts of copper ions were not removed(transported) from the macrophage, it was considered that intracellular excessive copper ions might cause DNA damage(DNA-strand breakage), leading to cell death.