Novel Factor Oxidized Galectin-1 Advances Functional Recovery after Peripheral Nerve Injury

我们发现氧化半乳糖凝集素-1在体外DRG外植体模型和体内周围神经轴突切开术模型中促进横断神经部位的轴突再生。本项目的目的是阐明氧化半乳糖凝集素-1是否促进周围神经损伤后神经功能的恢复。横断成年大鼠坐骨神经，用4根神经外针将远端神经固定于近端。通过渗透泵将氧化半乳糖凝集素-1或对照溶剂外周输送到手术部位。通过测量后爪脚趾伸展程度来评估功能恢复情况。恢复曲线显示氧化半乳糖凝集素-1明显促进功能恢复（与对照组相比，方差分析p<0.05）。组织学研究表明，氧化半乳糖凝集素-1增加了坐骨神经剪断后再生髓鞘轴突的数量。氧化半乳糖凝集素-1给药组有髓鞘轴突直径增大的趋势。这些结果表明，在神经损伤部位给予氧化半乳糖凝集素-1对神经功能的快速恢复是有效的。在此期间，我们已经完成了这个项目的目的，现在正准备发表一篇关于这项工作的论文。

We have revealed that oxidized galectin-1 promotes axonal regeneration from transected-nerve sites in an in vitro DRG explant model as well as in vivo peripheral nerve axotomy models. The purpose of this project is to clarify whether oxidized galectin-1 advances restoration of nerve functions after peripheral nerve injury.The sciatic nerve of an adult rat was transected and then the distal nerve was frozen after sutured into proximal site with four epineurial stitches. Peripheral delivery of oxidized galectin-1 or control solvent to the surgical site was performed with the osmotic pump. The functional recovery was evaluated by measuring the degree of toe spread of the hind paw. The recovery curves indicated that oxidized galectin-1 administration clearly advanced functional recovery (p<0.05 compared with control PBS group by ANOVA). The histological study showed that oxidized galectin-1 increased numbers of regenerating myelinated axons in a sciatic nerve after axotomy. There was the tendency that diameters of the myelinated axons were large in oxidized galectin-1 administrating group. These results indicate that administration of oxidized galectin-1 to the nerve injury sites is effective to the rapid restoration of the nerve function.We have performed the purpose of this project during its period and are now preparing to publish a paper of this work.