Immunomodulation with Endogenous Hormone in a Pleuronectiform Fish, the Barfin Flounder

鲽鱼中内源激素的免疫调节

• 批准号: 13660192
• 负责人: TAKAHASHI Akiyoshi
• 金额: $ 1.92万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660192/
• 关键词: Barfin flounder; Proopiomelanocrotin; Melanin-concentrating hormone; Brain; Pituitary; Skin; Appetite; Body color change; メラノトロピン; 下垂体; マクロファージ; エンドルフィン

Barfin flounder, Verasper moseri, is a member of Pleuronectiformes, inhabiting cold sea basin areas. This fish is promising for aquaculture in northern Japan. The present study was undertaken to clarify the relationship between an environmental factor (background color) and the physiological status, including immunomodulation. Of the barfin flounder.As a first step, we determined the primary structure of proopiomelanocortin (POMC), which is a common precursor of several peptide hormones responsible for immunomoduation and body color change, by cDNA cloning. A combination of reverse transcription-polymerase chain reaction and Southern blot analysis showed that the pituitary gland was the major tissue expressing POMC-A and -B genes. The expression of POMC-C gene was observed in all tissues examined In the pituitary, POMC-producing cells were identified in both pars distalis and pars intermedia by immunocytochemistry and in situ hybridization.Rearing of barfin flounder in a tank with a bl … More ack background resulted in higher expression of POMC-A, -B, and -C genes than those with a white background. The expression of POMC-C gene in the skin was higher in the black-reared fish than white-reared fish. These results indicate that background color modulates POMC expression in the pituitary and skin. Thus, body color change of barfin flounder is under the control of paracrine or autocrine systems in addition to endocrine control.Rearing of barfin flounder with a white background resulted in greater somatic growth than those in the black background tank. The expression of melanin-concentrating hormone (MCH), which has been identified as an appetite increasing hormone, was enhanced in the brain of white-reared fish and in fasted fish. Increased MCH in the brain of white background might have augmented the food intake.The effects of POMC-related peptides, which were synthesized based on the cDNA sequence, on barfin flounder leukocytes were also examined. No reproducibility was, however, obtained. Less

长鳍比目鱼，Verasper moseri，是胸鳍形的一员，生活在寒冷的海盆地区。这种鱼很有希望在日本北部进行水产养殖。本研究旨在阐明环境因素（背景色）与包括免疫调节在内的生理状态之间的关系。大比目鱼。作为第一步，我们通过cDNA克隆确定了proopiomelanocortin （POMC）的初级结构，POMC是负责免疫调节和身体颜色变化的几种肽激素的共同前体。逆转录聚合酶链反应和Southern blot分析表明垂体是POMC-A和-B基因的主要表达组织。在垂体中，通过免疫细胞化学和原位杂交，在远部和中部均发现了POMC-C基因的表达。背景较黑的比目鱼，其POMC-A、-B和-C基因的表达高于背景为白色的比目鱼。POMC-C基因在黑养鱼皮肤中的表达高于白养鱼。这些结果表明，背景颜色调节垂体和皮肤中POMC的表达。可见，牙鲆体色变化除受内分泌控制外，还受旁分泌或自分泌系统的控制。在白色背景下饲养的比目鱼比在黑色背景下饲养的比目鱼有更大的体生长。在白养鱼和禁食鱼的大脑中，黑色素浓缩激素（MCH）的表达得到了增强，该激素被认为是一种增加食欲的激素。白色背景的大脑中MCH的增加可能增加了食物的摄入量。研究了基于cDNA序列合成的pomc相关肽对棘牙鲆白细胞的影响。然而，没有获得可重复性。少

项目成果

Watanuki H, Sakai M, Takahashi, A: "Immunomodulatory effects of alpha melanocyte stimulating hormone on common carp (Cyprinus carpio L.)"Vet. Immunol. Immunopathol.. 91. 135-140 (2003)

Watanuki H、Sakai M、Takahashi、A：“α 黑素细胞刺激激素对鲤鱼 (Cyprinus carpio L.) 的免疫调节作用”兽医。

Takahashi A, Amemiya Y, Nozaki M, Sower SA, and Kawauchi H: "Evolutionary significance of proopiomelanocortin in agnathan and chondrichthyes"Comp.Biochem.Physiol.B. 129. 283-289 (2001)

Takahashi A、Amemiya Y、Nozaki M、Sower SA 和 Kawauchi H：“无颌类和软骨鱼中阿片黑皮素原的进化意义”Comp.Biochem.Physiol.B。

Takahashi A, Amemiya Y, Nozaki M, Sower SA, and Kawauchi H, Nobuyoshi Shimizu, Takashi Aoki, Ikuo Hirono, Fumio Takashima (eds.): "Molecular evolution of proopiomelanocrotin in fish"Aquatic Genomics, Springer. 217-224 (2003)

Takahashi A、Amemiya Y、Nozaki M、Sower SA 和 Kawauchi H、Nobuyoshi Shimizu、Takashi Aoki、Ikuo Hirono、Fumio Takashima（编辑）：“鱼类中阿片黑斑素原的分子进化”Aquatic Genomics，Springer。

Takahashi A, Amemiya Y, Yasuda A, Meguro H, Kawauchi H: "Mass spectrometric detection of proopiomelanocortin (POMC)-related peptides following molecular cloning of POMC cDNA in bigeye tuna, Thunnus obesus"Fish. Sci.. 68. 1073-1080 (2002)

Takahashi A、Amemiya Y、Yasuda A、Meguro H、Kawauchi H：“大眼金枪鱼、Thunnus obesus 中 POMC cDNA 分子克隆后阿片黑皮素原 (POMC) 相关肽的质谱检测”鱼。

Takahashi A, Amemiya Y, Nozaki M, Sower SA, Kawauchi H: "Aquatic Genomics"Nobuyoshi Shimizu, Takashi Aoki, Ikuo Hirono, Fumio Takashima. 432 (2003)

Takahashi A、Amemiya Y、Nozaki M、Sower SA、Kawauchi H：《水生基因组学》Nobuyoshi Shimizu、Takashi Aoki、Ikuo Hirono、Fumio Takashima。