Immunomodulation with Endogenous Hormone in a Pleuronectiform Fish, the Barfin Flounder

鲽鱼中内源激素的免疫调节

基本信息

批准号：
13660192
负责人：
TAKAHASHI Akiyoshi
金额：
$ 1.92万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

Barfin flounder, Verasper moseri, is a member of Pleuronectiformes, inhabiting cold sea basin areas. This fish is promising for aquaculture in northern Japan. The present study was undertaken to clarify the relationship between an environmental factor (background color) and the physiological status, including immunomodulation. Of the barfin flounder.As a first step, we determined the primary structure of proopiomelanocortin (POMC), which is a common precursor of several peptide hormones responsible for immunomoduation and body color change, by cDNA cloning. A combination of reverse transcription-polymerase chain reaction and Southern blot analysis showed that the pituitary gland was the major tissue expressing POMC-A and -B genes. The expression of POMC-C gene was observed in all tissues examined In the pituitary, POMC-producing cells were identified in both pars distalis and pars intermedia by immunocytochemistry and in situ hybridization.Rearing of barfin flounder in a tank with a bl … More ack background resulted in higher expression of POMC-A, -B, and -C genes than those with a white background. The expression of POMC-C gene in the skin was higher in the black-reared fish than white-reared fish. These results indicate that background color modulates POMC expression in the pituitary and skin. Thus, body color change of barfin flounder is under the control of paracrine or autocrine systems in addition to endocrine control.Rearing of barfin flounder with a white background resulted in greater somatic growth than those in the black background tank. The expression of melanin-concentrating hormone (MCH), which has been identified as an appetite increasing hormone, was enhanced in the brain of white-reared fish and in fasted fish. Increased MCH in the brain of white background might have augmented the food intake.The effects of POMC-related peptides, which were synthesized based on the cDNA sequence, on barfin flounder leukocytes were also examined. No reproducibility was, however, obtained. Less

Verasper Moseri的Barfin楼层是居住在冷海盆地区的胸膜甲状腺纤维肌的成员。这条鱼在日本北部有望成为水产养殖。本研究的目的是阐明环境因素（背景颜色）与身体状态（包括免疫调节）之间的关系。作为第一步，我们确定了蛋白质素皮质素（POMC）的主要结构，这是通过cDNA克隆来负责免疫调节和身体颜色变化的几种肽骑马的常见前体。逆转录聚合酶链反应和Southern印迹分析的结合表明，垂体是表达POMC-A和-b基因的主要组织。在垂体中检查的所有时间安排中都观察到了POMC-C基因的表达，通过免疫细胞化学和原位杂交在PARS远端和PARS Inmedia中都鉴定出了POMC产生的细胞。在黑色鱼类中，POMC-C基因在皮肤中的表达比白毛鱼高。这些结果表明背景颜色调制POMC在垂体和皮肤中的表达。这是Barfin地板的身体颜色变化，除了内分泌控制外，还在旁分泌或自分泌系统的控制下。与黑色背景储罐中的Barfin地板相比，Barfin地板具有白色背景的重新生长。黑色素浓缩的马酮（MCH）的表达已被确定为胃部增加的马龙，在白毛鱼和禁食鱼的大脑中得到了增强。在白色背景的大脑中，MCH的增加可能增加了食物的摄入量。还检查了基于cDNA序列合成的与POMC相关的肽对Barfin粉的白细胞的作用。但是，没有获得可重复性。较少的

项目成果

期刊论文数量（21）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Watanuki H, Sakai M, Takahashi, A: "Immunomodulatory effects of alpha melanocyte stimulating hormone on common carp (Cyprinus carpio L.)"Vet. Immunol. Immunopathol.. 91. 135-140 (2003)

Watanuki H、Sakai M、Takahashi、A：“α 黑素细胞刺激激素对鲤鱼 (Cyprinus carpio L.) 的免疫调节作用”兽医。

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Takahashi A, Amemiya Y, Nozaki M, Sower SA, and Kawauchi H: "Evolutionary significance of proopiomelanocortin in agnathan and chondrichthyes"Comp.Biochem.Physiol.B. 129. 283-289 (2001)

Takahashi A、Amemiya Y、Nozaki M、Sower SA 和 Kawauchi H：“无颌类和软骨鱼中阿片黑皮素原的进化意义”Comp.Biochem.Physiol.B。

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发表时间：
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0
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通讯作者：

Takahashi A, Amemiya Y, Nozaki M, Sower SA, and Kawauchi H, Nobuyoshi Shimizu, Takashi Aoki, Ikuo Hirono, Fumio Takashima (eds.): "Molecular evolution of proopiomelanocrotin in fish"Aquatic Genomics, Springer. 217-224 (2003)

Takahashi A、Amemiya Y、Nozaki M、Sower SA 和 Kawauchi H、Nobuyoshi Shimizu、Takashi Aoki、Ikuo Hirono、Fumio Takashima（编辑）：“鱼类中阿片黑斑素原的分子进化”Aquatic Genomics，Springer。